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E-raamat: Imaging Cell Signaling

  • Formaat: EPUB+DRM
  • Sari: Methods in Molecular Biology 2800
  • Ilmumisaeg: 06-May-2024
  • Kirjastus: Springer-Verlag New York Inc.
  • Keel: eng
  • ISBN-13: 9781071638347
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  • Formaat: EPUB+DRM
  • Sari: Methods in Molecular Biology 2800
  • Ilmumisaeg: 06-May-2024
  • Kirjastus: Springer-Verlag New York Inc.
  • Keel: eng
  • ISBN-13: 9781071638347

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This volume explores recent innovations across the entire pipeline of imaging signal transduction, cell preparation, cellular manipulation, image acquisition, and computational analysis. The chapters in this book cover topics such as rapid preparation of living Drosophila pupal macrophages for ex vivo imaging; controlling the potency of T cell activation using an optically tuneable chimeric antigen receptor; in situ imaging of proteins using DNA-PAINT super-resolution microscopy; reconstructing signaling networks using biosensor barcoding; and morphological, spatial, and dynamic models for cellular components. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Imaging Cell Signaling is a valuable resource that provides guidance to researchers looking to learn how to effectively tailor design projects to image, manipulate, and model signal transduction.


Rapid Preparation of Living Drosophila Pupal Macrophages for Ex Vivo
Imaging.- Generation of 3D Fibroblast-Derived Extracellular Matrix and
Analysis of Tumor Cell-Matrix Interactions and Signalling.- Induced Formation
of Plasma Membrane Protrusions with Porous Materials as Instructive
Surfaces.- Design and Synthesis of DNA Origami Nanostructures to Control TNF
Receptor Activation.- Controlling the Potency of T Cell Activation using an
Optically Tuneable Chimeric Antigen Receptor.- Imaging Cell Signaling in
Tissues using the IBEX Method.- Investigating Epidermal Growth Factor
Receptor Trafficking with High-Resolution Enzymatic Protein-Tagging and
Transmission Electron Microscopy.- Some Guiding Principles for a "Simple"
Correlative Light Electron Microscopy Experiment.- In Situ Imaging of
Proteins using DNA-PAINT Super-Resolution Microscopy.- A Magnetic Pincher for
the Dynamic Measurement of the Actin Cortex Thickness in Live
Cells.- Measurement of Forces Acting on Single T Cell Receptors.- A Pipeline
for Dynamic Analysis of Mitochondrial Content in Developing T Cells: Bridging
the Gap between High Throughput Flow Cytometry and Single-Cell Microscopy
Analysis.- Reconstructing Signaling Networks using Biosensor Barcoding.- Deep
Learning-Based Cell Tracking in Deforming Organs and Moving Animals.- Using
Dimensionality Reduction to Visualize Phenotypic Changes in High-Throughput
Microscopy.- Learning Morphological, Spatial, and Dynamic Models for Cellular
Components.