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E-raamat: Imaging and Spectroscopic Analysis of Living Cells: Imaging Live Cells in Health and Disease

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  • Sari: Methods in Enzymology
  • Ilmumisaeg: 30-Mar-2012
  • Kirjastus: Academic Press Inc
  • Keel: eng
  • ISBN-13: 9780123918680
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  • Formaat: PDF+DRM
  • Sari: Methods in Enzymology
  • Ilmumisaeg: 30-Mar-2012
  • Kirjastus: Academic Press Inc
  • Keel: eng
  • ISBN-13: 9780123918680
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This volume of Methods in Enzymology is the third of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike.

The second of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike.



This volume of Methods in Enzymology is the third of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike.

  • The third of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells
  • The chapters provide hints and tricks not available in primary research publications
  • It is an invaluable resource for academics, researchers and students alike

Muu info

This volume of Methods in Enzymology is the third of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells.
Contributors xiii
Preface xxi
Volumes in Series xxiii
Section I Viruses, Microbes, and Parasites
1(114)
1 Microbial Cells Analysis by Atomic Force Microscopy
3(16)
David Alsteens
1 Introduction
4(2)
2 Immobilization of Microbial Cells
6(5)
3 Applications of AFM to Nanomicrobiology
11(3)
4 Future Challenges
14(1)
Acknowledgments
14(1)
References
15(4)
2 Understanding Parasite Transmission Through Imaging Approaches
19(16)
Mirko Singer
Freddy Frischknecht
1 Why Imaging Parasites?
20(1)
2 In Vivo Models: "How Much Wrong Can Be Right?"
20(4)
3 The Choice of the Appropriate Microscope
24(1)
4 New Materials Provide the Basis for New Approaches to Dissect Cell Migration
25(2)
5 Visualizing Cellular Functions in Motile Cells
27(2)
6 Applying Imaging for Drug Discovery
29(2)
Acknowledgments
31(1)
References
31(4)
3 Multiphoton Microscopy Applied for Real-Time Intravital Imaging of Bacterial Infections In Vivo
35(28)
Ferdinand X. Choong
Ruben M. Sandoval
Bruce A. Molitoris
Agneta Richter-Dahlfors
1 Introduction
36(1)
2 Selection of Reporters for a Complex Site of Infection
37(4)
3 Setup of a Live Imaging Platform of Infection
41(9)
4 Studying Tissue Responses in Real-Time During Infection
50(7)
5 Postimaging Analysis
57(1)
6 Extending Real-Time Imaging into Molecular Analysis
58(2)
Acknowledgments
60(1)
References
60(3)
4 Analysis of Virus Entry and Cellular Membrane Dynamics by Single Particle Tracking
63(18)
Helge Ewers
Mario Schelhaas
1 Introduction
64(2)
2 Fluorescent Labeling of Viruses
66(7)
3 Data Acquisition and Analysis
73(5)
4 Conclusions and Outlook
78(1)
Acknowledgments
79(1)
References
79(2)
5 Imaging of Live Malaria Blood Stage Parasites
81(12)
Christof Gruring
Tobias Spielmann
1 Introduction
82(1)
2 Imaging Malaria Blood Stages at Ambient Temperature
83(3)
3 Long-Term 4D Imaging of P. falciparum Blood Stages
86(5)
Acknowledgments
91(1)
References
91(2)
6 Escherichia coli K1 Invasion of Human Brain Microvascular Endothelial Cells
93(22)
Lip Nam Loh
Theresa H. Ward
1 Introduction
94(1)
2 HBMEC Cell Culture and Transfection
95(6)
3 Making Fluorescent E. coli K1 that Retain Virulence
101(6)
4 Live Imaging of E. coli K1-Infected HBMEC
107(3)
Acknowledgments
110(1)
References
111(4)
Section II Disease States
115(80)
7 Real-Time Imaging After Cerebral Ischemia: Model Systems for Visualization of Inflammation and Neuronal Repair
117(18)
Pierre Cordeau
Jasna Kriz
1 Introduction
118(1)
2 Design and Generation of Dual Reporter Mouse Models for In Vivo Imaging of Brain Response to Ischemic Injury
119(5)
3 Induction of Cerebral Ischemia
124(1)
4 In Vivo Bioluminescence Imaging
125(5)
5 Additional Tips for Successful In Vivo Bioluminescence Imaging
130(2)
6 Summary
132(1)
Acknowledgments
132(1)
References
132(3)
8 In Vivo Real-Time Visualization of Leukocytes and Intracellular Hydrogen Peroxide Levels During a Zebrafish Acute Inflammation Assay
135(22)
Luke Pase
Cameron J. Nowell
Graham J. Lieschke
1 Introduction
136(1)
2 Visualizing Leukocyte Behavior During Acute Inflammation in Zebrafish
137(6)
3 Things to Consider When Planning In Vivo Visualization of the Inflammatory Response
143(11)
Acknowledgments
154(1)
References
154(3)
9 Imaging Protein Oligomerization in Neurodegeneration Using Bimolecular Fluorescence Complementation
157(18)
Federico Herrera
Susana Goncalves
Tiago Fleming Outeiro
1 Introduction
158(3)
2 Required Equipment and Materials
161(1)
3 Protocol
162(10)
Acknowledgements
172(1)
References
172(3)
10 3D/4D Functional Imaging of Tumor-Associated Proteolysis: Impact of Microenvironment
175(20)
Kamiar Moin
Mansoureh Sameni
Bernadette C. Victor
Jennifer M. Rothberg
Raymond R. Mattingly
Bonnie F. Sloane
1 Introduction
176(2)
2 Assays for Functional Imaging of Proteolysis
178(6)
3 3D/4D Models for Analysis of Biological Processes Linked to Proteolysis
184(2)
4 Live-Cell Imaging of MAME Models: A Screening Tool for Drug Discovery
186(3)
Acknowledgments
189(1)
References
189(6)
Section III Other Techniques
195(260)
11 Live Cell Imaging in Live Animals with Fluorescent Proteins
197(28)
Robert M. Hoffman
1 Introduction
199(2)
2 Imaging Angiogenesis In Vivo
201(2)
3 Imaging Cell Trafficking
203(6)
4 Methods
209(4)
5 Technical Details
213(3)
6 Imaging
216(2)
7 Chamber Imaging Systems
218(1)
8 Histological Techniques
219(1)
9 Conclusions
220(1)
References
221(4)
12 Protein Activation Dynamics in Cells and Tumor Micro Arrays Assessed by Time Resolved Forster Resonance Energy Transfer
225(22)
Veronique Calleja
Pierre Leboucher
Banafshe Larijani
1 Introduction
226(1)
2 Protein-Protein Interactions and Conformation Dynamics in Fixed and Live Cells
227(13)
3 Automated High-Throughput Analysis of Protein Activation in Tumor Micro Arrays
240(5)
Acknowledgments
245(1)
References
245(2)
13 Imaging Stem Cell Differentiation for Cell-Based Tissue Repair
247(18)
Zhenghong Lee
James Dennis
Jean Welter
Arnold Caplan
1 Introduction
248(2)
2 Identifying Marker Genes Associated with Stem Cell Differentiation
250(1)
3 Lenti-Viral Constructs for Event-Specific Reporter Gene Expression
251(3)
4 Applications of the Reporter System in Tissue Repair and Regeneration
254(7)
5 Concluding Remarks
261(1)
Acknowledgments
261(1)
References
261(4)
14 Understanding the Initiation of B Cell Signaling Through Live Cell Imaging
265(26)
Angel M. Davey
Wanli Liu
Hae Won Sohn
Joseph Brzostowski
Susan K. Pierce
1 Introduction
266(2)
2 Experiment Preparation
268(13)
3 Image Acquisition and Analysis
281(7)
Acknowledgments
288(1)
References
289(2)
15 A Quantitative Method for Measuring Phototoxicity of a Live Cell Imaging Microscope
291(20)
Jean-Yves Tinevez
Joe Dragavon
Lamya Baba-Aissa
Pascal Roux
Emmanuelle Perret
Astrid Canivet
Vincent Galy
Spencer Shorte
1 Introduction
292(1)
2 Measuring Phototoxicity
293(11)
3 Discussion
304(4)
4 Conclusion
308(1)
Acknowledgments
308(1)
References
308(3)
16 High Content Screening of Defined Chemical Libraries Using Normal and Glioma-Derived Neural Stem Cell Lines
311(20)
Davide Danovi
Amos A. Folarin
Bart Baranowski
Steven M. Pollard
1 Introduction
312(3)
2 Protocol: Chemical Screening Using Human NS and GNS Cells
315(5)
3 Protocol: Automated Quantitation of Cellular Responses
320(7)
Acknowledgments
327(1)
References
327(4)
17 High-Throughput Screening in Primary Neurons
331(30)
Punita Sharma
D. Michael Ando
Aaron Daub
Julia A. Kaye
Steven Finkbeiner
1 Introduction
332(1)
2 Sample Preparation
333(8)
3 Image Acquisition
341(5)
4 Data Analysis
346(6)
5 Statistical Approaches to HCS and Multivariate Data
352(4)
6 Future Directions
356(1)
7 Concluding Remarks
357(1)
Acknowledgments
358(1)
References
358(3)
18 Live Imaging Fluorescent Proteins in Early Mouse Embryos
361(30)
Panagiotis Xenopoulos
Sonja Nowotschin
Anna-Katerina Hadjantonakis
1 Introduction
362(1)
2 Genetically Encoded FPs for Live Imaging Morphogenetic Events in the Early Mouse Embryo
363(9)
3 Tools for Live Cell Imaging
372(6)
4 Methodology
378(6)
5 Conclusions
384(1)
Acknowledgments
385(1)
References
385(6)
19 Methods for Three-Dimensional Analysis of Dendritic Spine Dynamics
391(16)
Enni Bertling
Anastasia Ludwig
Mikko Koskinen
Pirta Hotulainen
1 Introduction
392(1)
2 Methods
393(2)
3 Imaging Conditions and Procedures
395(2)
4 Illustrative Experiments
397(1)
5 Quantitative Methods for Analyzing Dendritic Spines
398(6)
Acknowledgments
404(1)
References
404(3)
20 Imaging Cell Competition in Drosophila Imaginal Discs
407(8)
Shizue Ohsawa
Kaoru Sugimura
Kyoko Takino
Tatsushi Igaki
1 Introduction
407(2)
2 Live Imaging of Cell Competition
409(3)
References
412(3)
21 Live Cell Imaging of the Oviduct
415(10)
Sabine Kolle
1 Introduction
416(1)
2 Preparation of the Oviduct
417(2)
3 Live Cell Imaging: Qualitative Analysis
419(1)
4 Live Cell Imaging: Quantitative Analysis
420(3)
Acknowledgments
423(1)
References
423(2)
22 Computational Quantification of Fluorescent Leukocyte Numbers in Zebrafish Embryos
425(12)
Felix Ellett
Graham J. Lieschke
1 Introduction
426(1)
2 Computing LUs
427(6)
3 Example of Applicability: Enumerating Leukocyte Populations Following Perturbation of Macrophage/Neutrophil Specification by irf8 Misexpression
433(1)
Acknowledgments
433(1)
References
434(3)
23 Four-Dimensional Tracking of Lymphocyte Migration and Interactions in Lymph Nodes by Two-Photon Microscopy
437(18)
Masahiro Kitano
Takaharu Okada
1 Introduction
438(1)
2 Sample Preparation
439(5)
3 Imaging Preparations and Data Acquisition
444(3)
4 Data Analysis
447(5)
Acknowledgments
452(1)
References
453(2)
Author Index 455(24)
Subject Index 479
P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn served on the National Board of Medical Examiners, including two years as chairman of the reproduction and endocrinology committee. The work of his laboratory has been recognized with a MERIT award from the NIH, the J.J. Abel Award of the American Society for Pharmacology and Experimental Therapeutics, the Weitzman, Oppenheimer and Ingbar Awards of the Endocrine Society, the National Science Medal of Mexico (the Miguel Aleman Prize) and the Stevenson Award of Canada. He is the recipient of the Oregon State Award for Discovery, the Media Award of the American College of Neuropsychopharmacology and was named a distinguished Alumnus of Baylor College of Medicine in 2012. Conn is a previous member of Council for the American Society for Cell Biology and the Endocrine Society and is a prior President of the Endocrine Society, during which time he founded the Hormone Foundation and worked with political leadership to heighten the publics awareness of diabetes. Conns students and fellows have gone on to become leaders in industry and academia. He is an elected member of the Mexican Institute of Medicine and a fellow of the American Association for the Advancement of Science. He is the co-author of The Animal Research War (2008) and many articles for the public and academic community on the value of animal research and the dangers posed by animal extremism. His op/eds have appeared in The Washington Post, The LA Times, The Wall Street Journal, the Des Moines Register, and elsewhere. Conn consults with organizations that are influenced by animal extremism and with universities and companies facing challenges from these groups.