Contributors |
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xiii | |
Preface |
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xxi | |
Volumes in Series |
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xxiii | |
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Section I Viruses, Microbes, and Parasites |
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1 | (114) |
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1 Microbial Cells Analysis by Atomic Force Microscopy |
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3 | (16) |
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4 | (2) |
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2 Immobilization of Microbial Cells |
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6 | (5) |
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3 Applications of AFM to Nanomicrobiology |
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11 | (3) |
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14 | (1) |
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14 | (1) |
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15 | (4) |
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2 Understanding Parasite Transmission Through Imaging Approaches |
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19 | (16) |
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20 | (1) |
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2 In Vivo Models: "How Much Wrong Can Be Right?" |
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20 | (4) |
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3 The Choice of the Appropriate Microscope |
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24 | (1) |
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4 New Materials Provide the Basis for New Approaches to Dissect Cell Migration |
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25 | (2) |
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5 Visualizing Cellular Functions in Motile Cells |
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27 | (2) |
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6 Applying Imaging for Drug Discovery |
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29 | (2) |
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31 | (1) |
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31 | (4) |
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3 Multiphoton Microscopy Applied for Real-Time Intravital Imaging of Bacterial Infections In Vivo |
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35 | (28) |
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36 | (1) |
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2 Selection of Reporters for a Complex Site of Infection |
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37 | (4) |
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3 Setup of a Live Imaging Platform of Infection |
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41 | (9) |
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4 Studying Tissue Responses in Real-Time During Infection |
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50 | (7) |
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57 | (1) |
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6 Extending Real-Time Imaging into Molecular Analysis |
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58 | (2) |
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60 | (1) |
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60 | (3) |
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4 Analysis of Virus Entry and Cellular Membrane Dynamics by Single Particle Tracking |
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63 | (18) |
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64 | (2) |
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2 Fluorescent Labeling of Viruses |
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66 | (7) |
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3 Data Acquisition and Analysis |
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73 | (5) |
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4 Conclusions and Outlook |
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78 | (1) |
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79 | (1) |
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79 | (2) |
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5 Imaging of Live Malaria Blood Stage Parasites |
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81 | (12) |
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82 | (1) |
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2 Imaging Malaria Blood Stages at Ambient Temperature |
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83 | (3) |
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3 Long-Term 4D Imaging of P. falciparum Blood Stages |
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86 | (5) |
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91 | (1) |
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91 | (2) |
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6 Escherichia coli K1 Invasion of Human Brain Microvascular Endothelial Cells |
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93 | (22) |
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94 | (1) |
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2 HBMEC Cell Culture and Transfection |
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95 | (6) |
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3 Making Fluorescent E. coli K1 that Retain Virulence |
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101 | (6) |
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4 Live Imaging of E. coli K1-Infected HBMEC |
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107 | (3) |
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110 | (1) |
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111 | (4) |
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Section II Disease States |
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115 | (80) |
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7 Real-Time Imaging After Cerebral Ischemia: Model Systems for Visualization of Inflammation and Neuronal Repair |
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117 | (18) |
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118 | (1) |
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2 Design and Generation of Dual Reporter Mouse Models for In Vivo Imaging of Brain Response to Ischemic Injury |
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119 | (5) |
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3 Induction of Cerebral Ischemia |
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124 | (1) |
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4 In Vivo Bioluminescence Imaging |
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125 | (5) |
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5 Additional Tips for Successful In Vivo Bioluminescence Imaging |
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130 | (2) |
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132 | (1) |
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132 | (1) |
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132 | (3) |
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8 In Vivo Real-Time Visualization of Leukocytes and Intracellular Hydrogen Peroxide Levels During a Zebrafish Acute Inflammation Assay |
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135 | (22) |
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136 | (1) |
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2 Visualizing Leukocyte Behavior During Acute Inflammation in Zebrafish |
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137 | (6) |
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3 Things to Consider When Planning In Vivo Visualization of the Inflammatory Response |
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143 | (11) |
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154 | (1) |
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154 | (3) |
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9 Imaging Protein Oligomerization in Neurodegeneration Using Bimolecular Fluorescence Complementation |
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157 | (18) |
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158 | (3) |
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2 Required Equipment and Materials |
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161 | (1) |
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162 | (10) |
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172 | (1) |
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172 | (3) |
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10 3D/4D Functional Imaging of Tumor-Associated Proteolysis: Impact of Microenvironment |
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175 | (20) |
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176 | (2) |
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2 Assays for Functional Imaging of Proteolysis |
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178 | (6) |
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3 3D/4D Models for Analysis of Biological Processes Linked to Proteolysis |
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184 | (2) |
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4 Live-Cell Imaging of MAME Models: A Screening Tool for Drug Discovery |
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186 | (3) |
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189 | (1) |
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189 | (6) |
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Section III Other Techniques |
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195 | (260) |
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11 Live Cell Imaging in Live Animals with Fluorescent Proteins |
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197 | (28) |
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199 | (2) |
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2 Imaging Angiogenesis In Vivo |
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201 | (2) |
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3 Imaging Cell Trafficking |
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203 | (6) |
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209 | (4) |
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213 | (3) |
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216 | (2) |
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7 Chamber Imaging Systems |
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218 | (1) |
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8 Histological Techniques |
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219 | (1) |
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220 | (1) |
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221 | (4) |
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12 Protein Activation Dynamics in Cells and Tumor Micro Arrays Assessed by Time Resolved Forster Resonance Energy Transfer |
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225 | (22) |
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226 | (1) |
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2 Protein-Protein Interactions and Conformation Dynamics in Fixed and Live Cells |
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227 | (13) |
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3 Automated High-Throughput Analysis of Protein Activation in Tumor Micro Arrays |
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240 | (5) |
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245 | (1) |
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245 | (2) |
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13 Imaging Stem Cell Differentiation for Cell-Based Tissue Repair |
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247 | (18) |
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248 | (2) |
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2 Identifying Marker Genes Associated with Stem Cell Differentiation |
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250 | (1) |
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3 Lenti-Viral Constructs for Event-Specific Reporter Gene Expression |
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251 | (3) |
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4 Applications of the Reporter System in Tissue Repair and Regeneration |
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254 | (7) |
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261 | (1) |
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261 | (1) |
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261 | (4) |
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14 Understanding the Initiation of B Cell Signaling Through Live Cell Imaging |
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265 | (26) |
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266 | (2) |
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268 | (13) |
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3 Image Acquisition and Analysis |
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281 | (7) |
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288 | (1) |
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289 | (2) |
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15 A Quantitative Method for Measuring Phototoxicity of a Live Cell Imaging Microscope |
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291 | (20) |
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292 | (1) |
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2 Measuring Phototoxicity |
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293 | (11) |
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304 | (4) |
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308 | (1) |
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308 | (1) |
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308 | (3) |
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16 High Content Screening of Defined Chemical Libraries Using Normal and Glioma-Derived Neural Stem Cell Lines |
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311 | (20) |
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312 | (3) |
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2 Protocol: Chemical Screening Using Human NS and GNS Cells |
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315 | (5) |
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3 Protocol: Automated Quantitation of Cellular Responses |
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320 | (7) |
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327 | (1) |
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327 | (4) |
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17 High-Throughput Screening in Primary Neurons |
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331 | (30) |
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332 | (1) |
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333 | (8) |
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341 | (5) |
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346 | (6) |
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5 Statistical Approaches to HCS and Multivariate Data |
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352 | (4) |
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356 | (1) |
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357 | (1) |
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358 | (1) |
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358 | (3) |
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18 Live Imaging Fluorescent Proteins in Early Mouse Embryos |
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361 | (30) |
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Anna-Katerina Hadjantonakis |
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362 | (1) |
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2 Genetically Encoded FPs for Live Imaging Morphogenetic Events in the Early Mouse Embryo |
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363 | (9) |
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3 Tools for Live Cell Imaging |
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372 | (6) |
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378 | (6) |
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384 | (1) |
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385 | (1) |
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385 | (6) |
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19 Methods for Three-Dimensional Analysis of Dendritic Spine Dynamics |
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391 | (16) |
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392 | (1) |
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393 | (2) |
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3 Imaging Conditions and Procedures |
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395 | (2) |
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4 Illustrative Experiments |
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397 | (1) |
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5 Quantitative Methods for Analyzing Dendritic Spines |
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398 | (6) |
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404 | (1) |
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404 | (3) |
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20 Imaging Cell Competition in Drosophila Imaginal Discs |
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407 | (8) |
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407 | (2) |
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2 Live Imaging of Cell Competition |
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409 | (3) |
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412 | (3) |
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21 Live Cell Imaging of the Oviduct |
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415 | (10) |
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416 | (1) |
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2 Preparation of the Oviduct |
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417 | (2) |
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3 Live Cell Imaging: Qualitative Analysis |
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419 | (1) |
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4 Live Cell Imaging: Quantitative Analysis |
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420 | (3) |
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423 | (1) |
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423 | (2) |
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22 Computational Quantification of Fluorescent Leukocyte Numbers in Zebrafish Embryos |
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425 | (12) |
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426 | (1) |
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427 | (6) |
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3 Example of Applicability: Enumerating Leukocyte Populations Following Perturbation of Macrophage/Neutrophil Specification by irf8 Misexpression |
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433 | (1) |
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433 | (1) |
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434 | (3) |
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23 Four-Dimensional Tracking of Lymphocyte Migration and Interactions in Lymph Nodes by Two-Photon Microscopy |
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437 | (18) |
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438 | (1) |
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439 | (5) |
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3 Imaging Preparations and Data Acquisition |
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444 | (3) |
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447 | (5) |
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452 | (1) |
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453 | (2) |
Author Index |
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455 | (24) |
Subject Index |
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479 | |