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E-raamat: Immunocytochemistry and Related Techniques

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  • Formaat: EPUB+DRM
  • Sari: Neuromethods 101
  • Ilmumisaeg: 24-Feb-2015
  • Kirjastus: Humana Press Inc.
  • Keel: eng
  • ISBN-13: 9781493923137
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  • Formaat: EPUB+DRM
  • Sari: Neuromethods 101
  • Ilmumisaeg: 24-Feb-2015
  • Kirjastus: Humana Press Inc.
  • Keel: eng
  • ISBN-13: 9781493923137
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This volume presents a collection of protocols for immunocytochemical analysis of neurons and neural networks. Chapters focus on immunocytochemical localization at light and electronic levels, biochemical characterization, and functional analysis in vivo or ex vivo by novel types of microscopy, as well as protocols for development and production of genetic probes. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.

Essential and authoritative, Immunocytochemistry and Related Techniques is intended for a large audience of scientists, including histologists, biochemists, cellular and molecular biologists, electrophysiologists that are currently active in the field or are willing to enter the exciting area of neuroscience research.

Series Preface v
Preface vii
Contributors xi
1 The Evolution of Immunocytochemistry in the Dissection of Neural Complexity
1(38)
Adalberto Merighi
Laura Lossi
Part I Immunocytochemistry in Non-Mammalian Brain
2 Immunofluorescence and Genetic Fluorescent Labeling Techniques in the Drosophila Nervous System
39(24)
Mareike Selcho
Christian Wegener
3 Immunohistochemical Analysis of Neuronal Networks in the Nervous System of Octopus vulgaris
63(18)
Giovanna Ponte
Graziano Fiorito
4 Genetic Mosaic Labeling and Immunofluorescence Techniques in Zebrafish Brain
81(14)
Nobuhiko Miyasaka
Noriko Wakisaka
Yoshihiro Yoshihara
Part II Detection of Cell Proliferation and Neurogenesis
5 Immunocytochemical Phenotype of Differentiating Neurons
95(14)
Andrea Diana
Antonio Carai
6 Immunocytochemical Identification of Mammalian Differentiating Neurons in the Process of Adult Neurogenesis in the Hippocampal Dentate Gyrus
109(14)
Oliver von Bohlen und Halbach
7 Identification of Proliferating and Migrating Cells by BrdU and Other Thymidine Analogs: Benefits and Limitations
123(18)
Alvaro Duque
Pasko Rakic
8 Flow Cytometry-Based Quantification of Neurogenesis in the Central Nervous System
141(12)
Armin Schneider
Part III Identification of Specific Cell Types and Intracellular Pathways
9 In Vivo and In Vitro Immunohistochemical Visualization of Neural Cell Apoptosis and Autophagy
153(26)
Maria Grazia Bottone
Francesco Paolo Fanizzi
Graziella Bernocchi
10 Immunocytochemical Detection of Intraneuronal Aβ Peptides in Mouse Models of Alzheimer's Disease
179(16)
Oliver Wirths
Anika Saul
11 Purification and Characterization of Mouse Olfactory Ensheathing Cells and Their Potential Use for Therapy in Amyotrophic Lateral Sclerosis (ALS)
195(14)
Chrystian Junqueira Alves
Alexandre Baldasserini Guimaraes
Jessica Ruivo Maximino
Sergio Paulo Bydlowski
Gerson Chadi
12 Immunocytochemistry of Microglial Cells
209(16)
Dmitrii E. Korzhevskii
Olga Kirik
Elena Sukhorukova
13 Immunocytochemical Assessment of Blood-Brain Barrier Structure, Function, and Damage
225(32)
Jean-Pierre Louboutin
Part IV Quantification of Immunocytochemistry and Combined Techniques
14 Quantification of Immunocytochemical Colocalization in Neurons
257(24)
Brad R. Rocco
Kenneth N. Fish
15 Immunogold Electron Microscopic Quantification of Small Molecular Compounds and Proteins at Synapses and Other Neural Profiles
281(18)
Max Larsson
Linda Hildegard Bergersen
Vidar Gundersen
16 Combined Immunocytochemistry and Tracing of Neural Connections
299(14)
Taisuke Miyazaki
Masahiko Watanabe
17 Neuronal Circuitry Dissected by Immunocytochemistry Combined with Retrograde Tracing and Electrophysiology
313(16)
Alberto Granato
Andrea De Giorgio
18 Transfection Techniques and Combined Immunocytochemistry in Cell Cultures and Organotypic Slices
329(28)
Silvia Alasia
Adalberto Merighi
Laura Lossi
19 Combined Immunochemistry and Live Imaging of Fluorescent Protein Expressing Neurons in Mouse Brain
357(20)
Ruth M. Empson
Malinda L.S. Tantirigama
Manfred J. Oswald
Stephanie M. Hughes
Thomas Knopfel
Part V Novel Approaches
20 Array Tomography: A Novel High-Resolution Immunofluorescence Technique
377(12)
Mariano Soiza-Reilly
21 Single Molecule Localization Microscopy to Study Neuronal Microtubule Organization
389(20)
Bas M.C. Cloin
Casper C. Hoogenraad
Marina Mikhaylova
Lukas C. Kapitein
22 Studying Membrane Dynamics of Proteins Using Quantum Dots
409(14)
Martin Heubl
Sabine Levi
23 Optical Protein Patterning
423(14)
Santiago Costantino
Index 437