This volume presents the latest collection of immunophenotypic techniques and applications used in research and clinical settings. Chapters in this book cover topics such as constructions of high dimensions fluorescence and mass cytometry panels; fluorescence barcoding; using dried or lyophilized reagents; and immunophenotypic examples of specific cell types. The book concludes with a discussion on the critical roles of quality control and immunophenotyping in the clinical environment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Immunophenotyping: Methods and Protocols is a valuable resource for any researchers, clinician, or scientist interested in learning more about this evolving field.
High Dimensional Immunophenotyping with Fluorescence-based Cytometry: A
Practical Guidebook.- Immunophenotyping by Mass Cytometry.- Fluorescent Cell
Barcoding for Immunophenotyping.- Immunophenotyping using Dried and
Lyophilized Reagents.- Guidelines for Gating Flow Cytometry Data for
Immunological Assays.- Strategies and Techniques for NK Cell
Phenotyping.- Immunophenotyping of Human B Lymphocytes in the Blood and in
the Adipose Tissue.- Multiparametric Flow Cytometry Analysis of Naïve,
Memory, and Effector T Cells.- Immunophenotyping of Human Regulatory T
Cells.- Immunophenotyping of Human Innate Lymphoid Cells.- Enumeration of
Plasmacytoid Dendritic Cells in Peripheral Blood and Bone Marrow by Flow
Cytometric Analysis.- Immunophenotyping of Circulating Endothelial Cell and
Endothelial Microparticles.- Rare Event Phenotyping and Molecular
Characterization: Circulating Tumor Cells.- Quality Control of
Immunophenotyping.- Immunophenotyping of Acute Myeloid
Leukemia.- Immunophenotyping of Acute Lymphoblastic Leukemia.- Clinical Flow
Cytometry Testing in Chronic Lymphocytic Leukemia.- Immunophenotyping of
Paroxysmal Nocturnal Hemoglobinuria (PNH).
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