This third edition volume expands on the previous editions with new chapters discussing advancements in mass spectrometry resolution, sensitivity, and speed, including the refinement of the identification of peptides presented on the surface of major histocompatibility proteins. These technologies continue to advance vaccine development, as well as cancer immunotherapy and precision medicine. Furthermore, this new edition introduces new approaches for quantifying immune-active proteins and peptides and explores the increasing role of multi-omics strategies to help researchers further understand immune function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Immunoproteomics: Methods and Protocols, Third Edition will help advance immune system research and support the development of new diagnostic and therapeutic strategies.
Introduction to the Immune System.- Immunoproteomics: The Evolution from
Classical Methods to Emerging Techniques.- Serological Proteome Analysis for
Antigen Identification.- Targeted Analyses of MHC-I Peptides using TOMAHAQ.-
High-Coverage Immunopeptidomics Using timsTOF Mass Spectrometers with
Thunder-DDA-PASEF Boosted by MS2Rescore.- Identification of Antibody-Cognate
Antigens via a High-Throughput, Affinity-Chromatography-Based, Shotgun
Immunoproteomics Pipeline.- Identification of Disease Characteristic Immune
Complex Antigens.- Enrichment of Phosphorylated and Glycosylated MHC Peptides
for Mass Spectrometry-Based Neoantigen Analysis.- Characterization of Human
Leukocyte Antigen (HLA) Proteins with Integrated Immunopeptidomics and
Proteomics Approach.- Artemis: Mass Spectrometry-Based Identification of MHC
Presented Peptides Across Alleles, Classes, and Species using Soluble
Single-Chain MHC Constructs.- A Novel Machine Learning Post-Processing Filter
for Mass Spectrometry-Based Proteogenomics Leveraging Retention Time
Deviation and Peptide Physiochemical Properties.- Utilizing Protein
Bioinformatics to Delve Deeper into Immunopeptidomic Datasets with AIMS: An
Automated Immune Molecule Separator.- Ribosome Profiling for Novel Antigen
Discovery.- Multiplexed Detection of Autoantibodies to Glycopeptides Using
Microarray.- Preparation of the Low Molecular Weight Serum Proteome for Mass
Spectrometry Analysis.- MHCII MAPPS for High-Confident Immunogenicity Risk
Assessment of Biotherapeutics.- FLEXMAP 3D Immunoproteomics: A Protocol to
Characterize General Human Antibody Profiles Against Pathogens.- Profiling
Norovirus Antibody Specificity and Function Using Luciferase
Immunoprecipitation System (LIPS) Assays.- Nucleic Acid Programmable Protein
Arrays for Autoantibodies Discovery, A Step-by-Step Guide.- Epitope Screening
by Hydrogen-Deuterium Exchange Mass Spectrometry for Improved
Immunotherapeutic Outcomes.- Intact Mass Spectrometry Analysis of
Immuno-Isolated Human Therapeutic Antibodies from Serum.- Prioritization of
Therapeutic Targets of Inflammation Using Proteomics, Bioinformatics, and In
Silico Cell-Cell Interactomics.
Lisainfo e-raamatute kohta