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E-raamat: Immunoproteomics: Methods and Protocols

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Immunoproteomics: Methods and ProtocolsSuurem pilt
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This second edition volume expands on the previous edition with new sections describing the characterization of peptides bound to major histocompatibility complexes (MHC) on the surface of the cell. Chapters also cover topics such as using SERPA for antigen identification; antigen content of electroimmunoprecipitates; whole genome-phage display libraries; antigens in immune complexes; and immunoproteomic biomarkers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Cutting-edge and thorough, Immunoprotemics: Methods and Protocols, Second Edition is a valuable resource that presents novice and expert researchers with techniques that are easily transferrable to laboratories and provides enhanced hands-on insights into this evolving field. 

Introduction to the Immune System.- Immunoproteomics Methods and
Techniques.- Classical Immunoproteomics: Serological Proteome Analysis
(SERPA) for Antigen Identification.- Profiling of Cytokine and Chemokine
Responses using Multiplex Bead Array Technology.- Preparation of the Low
Molecular Weight Serum Proteome for Mass Spectrometry Analysis.- Enriching
for Low Abundance Serum Proteins using ProteoMiner and Protein Level
HPLC.- Identification of the Antigen Content of
Electroimmunoprecipitates.- Detection of Human Norovirus-Specific Antibodies
using the Luciferase Immunoprecipitation System (LIPS) Assay.- Intact Mass
Spectrometry Analysis of Immuno-Isolated Human Therapeutic Antibodies from
Serum.- Antigen Identification for Cell-Binding Antibodies using Ligand
Directed Crosslinking and Biotin Transfer.- Whole Genome-Phage Display
Libraries: A Powerful Tool for Antigen Discovery.- Multiplexed Detection of
Autoantibodies to Glycopeptides using Microarray.- Construction and Screening
of an Antigen-Derived Peptide Library Displayed on Yeast Cell Surface for
CD4+ T Cell Epitope Identification.- Isolation of Major Histocompatibility
Complex (MHC)-Associated Peptides by Immunoaffinity Purification.- Enhanced
Mass Spectrometry Detection of MHC Peptides.- Enrichment of Phosphorylated
MHC Peptides with Immobilized Metal Affinity Chromatography and Titanium
Dioxide Particles.- Mass Spectrometric Identification and Molecular Modeling
of Glycopeptides Presented by MHC Class I and II Processing Pathways.- Genome
Based Bioinformatic Prediction of Major Histocompatibility (MHC).- Enhancing
Mass Spectrometry-Based MHC-I Peptide Identification through a Targeted
Database Search Approach.- Prioritization of Therapeutic Targets of
Inflammation Using Proteomics, Bioinformatics, and In Silico Cell-Cell
Interactomics.- Genomics Driven Immunoproteomics: An Integrative Platform to
Uncover Important Biomarkers for Human Diseases.- Identification of Antigens
in Immune Complexes.- Analysis and Characterization of Immune Cells and their
Activation Status by Whole-Cell MALDI TOF Mass Spectrometry.- A Molecular
Immunoproteomics Approach to Assess the Viral Antigenicity of
Influenza.- Immunoproteomic Biomarkers: From Publication to Personalized
Medicine.
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