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E-raamat: Insect Genomics: Methods and Protocols

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Insect Genomics: Methods and ProtocolsSuurem pilt
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This collection explores advances in genome sequencing and analysis tools for the understanding of insect genomes and hence their biology. Beginning with obtaining a high-quality genome assembly using the latest sequencing data, the book continues with the identification of genome variability including single nucleotide polymorphisms and genomic inversions, and the annotation of insect genomes through genes, transposable elements, and piRNA clusters. Notably, chapters also delve into downstream analyses, such as the evolution of gene families and phylogenetics, molecular biology protocols pertinent to the study of insects, as well as CRISPR-era genome editing tools to manipulate non-model insects. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice to ensure success in the laboratory.

 

Authoritative and practical, Insect Genomics: Methods and Protocols serves as an ideal source for state-of-the-art methods and comprehensive reviews to help researchers design and implement their own studies.

De Novo Genome Assembly Using Long Reads and Chromosome Conformation
Capture.- Identification of Single Nucleotide Polymorphism from Insect
Genomic Data.- npstat: An Efficient Tool to Explore the Population Genome
Variability and Divergence Using Pool Sequencing Data.- Navigating Eukaryotic
Genome Annotation Pipelines: A Route Map to Using BRAKER, Galba, and TSEBRA.-
A Concise Guide for the Characterization and Curation of Transposable
Elements in Insect Genomes.- Annotation of piRNA Source Loci in the Genome of
Non-Model Insects.- The Influence of Chromosomal Inversions on Genetic
Variation and Clinal Patterns in Genomic Data of Drosophila melanogaster.-
Evolutionary Genomics of Gene Families: A Case Study of Insect Gustatory
Receptors.- Insect Phylogenomics: From Experiment Planning to
Post-Phylogenetic Analyses.- High-Throughput Amplicon Sequencing for
Analyzing Microbial Communities of Insects.- An Optimized SPLiT-Seq Protocol
for Insects.- Identification of Sex-Specific and Sex-Biased Transcripts for
Genetic Sexing.- Characterization of Mosquito Salivary Components.- History
of Mosquito Transgenesis: A Perspective in Review.- Manipulation of a New
Non-Model Insect Genome Using Targeted CRISPR-Era Approaches.- Adapting and
Testing ReMOT Control for Expanded CRISPR-Era Genome Functions in Non-Model
Insects.
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