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E-raamat: Introduction to Molecular Ecology

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(Lecturer in Biological Sciences, University of Derby), (Lecturer in Invertebrate Biology, University of Derby), (Emeritus Professor (Evolution, Behaviour and Environment), University of Sussex)
  • Formaat: 399 pages
  • Ilmumisaeg: 30-Mar-2017
  • Kirjastus: Oxford University Press
  • Keel: eng
  • ISBN-13: 9780192535535
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Introduction to Molecular EcologySuurem pilt
  • Formaat: 399 pages
  • Ilmumisaeg: 30-Mar-2017
  • Kirjastus: Oxford University Press
  • Keel: eng
  • ISBN-13: 9780192535535
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An Introduction to Molecular Ecology combines theoretical concepts with practice-driven examples to showcase the latest molecular and genomic techniques and their impact on the study of ecology.

The opening chapters introduce the essential molecular and genetic concepts that underpin the subject and describe key molecular tools and methods available to the ecologist. Capturing the broad scope of the field, the book goes on to explore the use of molecular tools in the context of behavioural ecology, population genetics, phylogeography, conservation, and microbial ecology.

The third edition includes coverage of exciting new technological and analytical developments, such as next generation sequencing, which have revolutionized the field over the last decade. Molecular ecology is now in a position to tackle some truly ecological questions for the first time since its inception.

Blending conceptual detail with the most instructive examples, An Introduction to Molecular Ecology is an ideal resource for those new to the subject needing to develop a strong working understanding of the field.

Online Resource Centre

The Online Resource Centre to accompany An Introduction to Molecular Ecology features: For students: - Suggested review articles to take your learning further - Links to useful websites and software - A range of questions to consolidate your learning and understanding

For registered adopters of the book: - Journal Club; suggested research papers and discussion questions linked to topics featured in the book - Figures from the textbook to view and download

Arvustused

The authors of the third edition of this well-established textbook have achieved the near impossible. They encapsulate with clarity and generous illustration the breadth and excitement of contemporary molecular ecology, with substantive updates that now embrace fully the omics era. * Professor Gary R. Carvalho, Bangor University * This thoroughly revised and updated edition is a major advance for the field of molecular ecology. It rises to the challenge of explaining new developments and technologies with flair and clarity, and does an excellent job of synthesizing and presenting the latest results to a new generation of molecular ecologists. It is a superb teaching tool and one I plan to use when I next teach molecular ecology. * Professor Scott V. Edwards, Harvard University * This is a great text, providing a comprehensive and detailed explanation of how technological advances in molecular biology are used to answer questions in ecology. Students and faculty alike will find this a wonderful study tool and resource. * Dr Sue Hum-Musser, Western Illinois University * This book is clear, accessible, and engaging, and is perfectly suited for undergraduates. With new chapters on next generation sequencing and genomics, the third edition is an up-to-date resource for teaching and learning about molecular ecology. * Dr Nusha Keyghobadi, Department of Biology, Western University *

Preface ix
Acknowledgements x
1 Molecular ecology: roots and branches
1(25)
What is molecular ecology?
1(3)
Overview and organization of this book
4(1)
Evolutionary framework for molecular ecology
5(8)
Molecules, molecular markers, and molecular ecology
13(4)
Next generation molecular ecology
17(5)
Putting the ecology back into molecular ecology
22(2)
Summary
24(2)
2 Molecular biology for ecologists
26(38)
Introduction
26(1)
Nucleic acids and the common origin of life
27(1)
Structure of DNA and RNA
28(4)
Mutation as the source for all genetic variation
32(3)
Accessing DNA sequence information
35(8)
DNA to proteins
43(4)
Proteins
47(2)
Genome organization and genomics
49(2)
Eukaryotic genome structure
51(1)
Non-coding DNA
52(3)
Coding and other functional genomic regions
55(4)
Gene expression, the transcriptome, and proteome
59(3)
Summary
62(2)
3 Molecular tools for molecular ecologists
64(27)
Introduction
64(2)
Genetic manipulation in molecular ecology
66(2)
Protein and DNA extraction from tissue samples
68(3)
Non-PCR-based tools for studying genetic variation
71(5)
Accessing the genome using PCR-based molecular markers
76(1)
PCR-based molecular markers requiring sequence information from the study species
77(6)
PCR-based molecular markers NOT requiring sequence information from the study species
83(4)
Measuring gene expression
87(2)
Next generation sequencing and the marker revolution
89(1)
Summary
89(2)
4 Next generation sequencing
91(32)
Introduction
91(1)
First, second, and third generation sequencing
92(3)
Next generation sequencing platforms
95(5)
Methodological advancements in next generation sequencing
100(2)
Next generation sequencing applications in molecular ecology
102(2)
Deep sequencing
104(2)
Targeted sequencing
106(2)
Marker discovery and genotyping
108(3)
Transcriptomics
111(4)
Epigenomics
115(4)
Next generation sequencing: a panacea for molecular ecology?
119(3)
Summary
122(1)
5 Species, populations, and individuals
123(42)
Introduction
123(1)
The species problem
124(6)
Hybrids and hybrid zones
130(4)
Evolutionary significant units and management units
134(2)
Cryptic species, sibling species, and sister species
136(3)
DNA barcoding, metabarcoding, and metagenomics
139(5)
Environmental DNA
144(3)
Ancient DNA and proteins
147(6)
Dealing with individuals
153(4)
Identification of sex
157(3)
Bits of individuals: non-invasive sampling
160(3)
Summary
163(2)
6 Behavioural ecology
165(41)
Introduction
165(1)
From monogamy to promiscuity
166(9)
Sexual dimorphism and male dominance
175(3)
Female reproductive behaviour
178(5)
Sex ratio biases in offspring
183(1)
Cooperative behaviour
184(3)
Cheating tactics
187(2)
Foraging and dispersal
189(3)
Behaviourally mediated speciation
192(6)
Genomes, genomics, and animal behaviour
198(2)
Epigenetics and behavioural ecology
200(3)
Current direction and future challenges for behavioural ecology
203(1)
Summary
204(2)
7 Population genetics
206(37)
Introduction
206(1)
Genetic diversity in natural populations
207(7)
Effective population size
214(4)
Population bottlenecks and expansions
218(3)
Population structure
221(6)
Genetics of metapopulations
227(4)
Gene flow and migration rates
231(5)
Identification of immigrants
236(2)
Landscape genetics: bringing it all together
238(2)
Molecular markers for population genetics: an appraisal
240(2)
Summary
242(1)
8 Neutral and adaptive molecular variation
243(35)
Introduction
243(2)
Genetic variation in populations
245(2)
Genetic load, natural selection, and the neutral theory
247(4)
Neutral and adaptive variation
251(5)
Neutral markers which are not completely neutral
256(4)
Heterozygosity and fitness
260(5)
Molecular approaches to understanding adaptive variation
265(8)
Genomics and the study of adaptive variation
273(4)
Summary
277(1)
9 Phylogeography
278(44)
Introduction
278(1)
Molecular markers in phylogeography
279(8)
Genetic variation in space
287(17)
Genetic variation in time
304(5)
Phylogeography of microorganisms
309(2)
Applied phylogeography
311(9)
Summary
320(2)
10 Conservation genetics
322(33)
Introduction
322(2)
Molecular genetics in conservation biology
324(2)
Genetic diversity as a conservation issue
326(4)
Inbreeding and genetic load
330(6)
Genetic restoration
336(2)
Captive breeding for reintroduction into the wild
338(1)
Desperate measures
339(5)
Wildlife health and disease
344(3)
Wildlife forensics
347(1)
Genetics in conservation biology---a wider role
348(6)
Summary
354(1)
11 Microbial ecology and metagenomics
355(37)
Microbial diversity and metagenomes
355(3)
Outstanding issues in microbial ecology
358(4)
Immunological approaches to microbial ecology
362(4)
Ribosomal sequences and microbial ecology: start of metagenomics
366(12)
Genomic approaches to microbial ecology
378(4)
Bacteria and the concept of species
382(4)
Viral diversity
386(1)
Phylogeography
387(2)
Overview of microbial molecular ecology
389(1)
Summary
390(2)
12 Genomes
392(42)
Introduction
392(5)
First genome sequences
397(2)
Human Genome Project and competing sequencing strategies for eukaryotic genomes
399(4)
Depth and breadth of genome sequencing
403(2)
Genome reassembly and analysis
405(8)
Genome annotation
413(1)
Whole genomes or complete genomes?
414(1)
Diploid genome sequences
415(1)
Genome sequences of non-model organisms
416(13)
Microsomes
429(3)
Summary
432(2)
13 Genomics
434(38)
Introduction
434(1)
Comparative genomics
435(3)
Genome architecture and gene expression
438(4)
Evolutionary genomics
442(1)
Phylogenomics
443(3)
Genetic variability
446(2)
Population genomics
448(1)
Conservation genomics
449(2)
Adaptation
451(1)
Speciation
452(4)
Transcriptomics
456(1)
Proteomics
457(4)
Metabolomics
461(1)
Epigenomics
462(1)
Genome editing
463(6)
Molecular ecology comes of age
469(1)
Summary
470(2)
References 472(47)
Index 519
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