The Laboratory Manual of Reproductive Endocrinology and Assisted Reproductive Technology serves as a comprehensive, step-by-step guide for undergraduate and postgraduate students of zoology, life sciences, biotechnology and the medical sciences. It integrates fundamental concepts with advanced experimental approaches, beginning with animal handling and histological examinations and progressing to modern techniques such as hormonal assays, protein profiling, oocyte retrieval, embryo grading and infertility investigations.
Detailed coverage of assisted reproductive technologies (ART) including in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI), cryopreservation and embryo transfer is complemented by discussions on ethics, frameworks and the role of artificial intelligence (AI) in reproductive health. To support effective learning, the manual incorporates illustrations, labeled images, video guide, a glossary and references, making it an invaluable resource for both classroom teaching and online learning. Scientifically rigorous and practically oriented, it equips learners with conceptual clarity, technical skills and ethical insight into the evolving field of reproductive biology.
Key Features:
- Step-by-step coverage of both basic and advanced experimental procedures in reproductive biology and endocrinology
- Detailed modules on ART, including IVF, ICSI, embryo grading and cryopreservation
- Integration of contemporary issues such as ethics and AI in reproductive health
- Rich visual aids, including labeled images, illustrations, histological sections and a step-by-step surgical video guide, to support effective learning
- Glossary of key terms and references to relevant literature for further study
The manual Reproductive Biology, Endocrinology and Assisted Reproductive Technology serve as a comprehensive, step-by-step guide for undergraduate and postgraduate students of Zoology, Life Sciences, Biotechnology, and Medical Sciences.
Chapter 1: Set up and maintenance of Rodent Animal House facility;
Chapter 2: Display of major endocrine glands and reproductive organ system in
male and female rats;
Chapter 3: Microscopic examinations of histological
sections using illustrations/permanent slides/photomicrographs of human/rat;
Chapter 4: Surgical techniques: Principles of surgery in endocrinology;
Chapter 5: Dissect and display of the hypothalamic-hypophyseal organ system
in rats;
Chapter 6: To study the estrous cycle by observing the vaginal smear
of adult female rats (Sprague-Dawley) under normal conditions;
Chapter 7:
Study of Exfoliative cytology of human cervix and vagina through micrographs;
Chapter 8: Study of Sperm counting, Sperm Vitality and Sperm Motility in
rats;
Chapter 9: Study the Effect of Cryptorchidism on Sperm Count and
Motility in Rats;
Chapter 10: Study of Protein Profile of Rat Ovarian Extract
by SDS-PAGE;
Chapter 11: Separation of steroid hormones by Thin layer
Chromatography and Paper chromatography;
Chapter 12: To determine and
quantify glycogen/ascorbic acid levels in rat uterine tissue;
Chapter 13: To
determine and quantify fructose in the dorsolateral prostate and coagulating
glands of rat;
Chapter 14: To Estimate Total Estrogens in Human Serum Samples
Using Competitive ELISA technique;
Chapter 15: To Understand the Methodology
of a Pregnancy Test Kit;
Chapter 16: Birth control methods: Study of modern
contraceptive devices;
Chapter 17: Retrieval of oocytes by induced
superovulation in immature mice;
Chapter 18: Study of causes of infertility
in males and females and infertility investigation using secondary data,
models and pictographs;
Chapter 19: Setting up and Study of IVF Laboratory
Culture Conditions;
Chapter 20: Oocyte scoring and embryo quality grading in
IVF for successful implantation;
Chapter 21: Study of Assisted Reproductive
Techniques;
Chapter 22: Assisted Reproductive Technology: Ethical principles
and laws in India;
Chapter 23: Artificial Intelligence tools for Detection
and Management of Sexually Transmitted Infections and Women's Reproductive
Health: A Practical Approach for Undergraduate Students.
Jyoti Taneja is an Assistant Professor, with over 15 years of teaching experience in the Department of Zoology at Daulat Ram College, University of Delhi. Her research focuses on reproductive health, infectious disease epidemiology, and the application of machine learning in disease biology. She has a particular interest in polycystic ovary syndrome (PCOS), menstrual health, and reproductive tract infections, exploring how lifestyle factors influence womens reproductive well-being.
In addition to her work in reproductive health, Dr. Taneja applies AI-driven methods to improve diagnostic accuracy and support vaccine development. Her expertise in infectious disease epidemiology has been pivotal in understanding the dynamics of COVID-19, including disease severity, breakthrough infections, post-COVID sequelae, and vaccine hesitancy. Her research has contributed significantly to evidence-based public health policies and interventions, and is widely published in leading scientific journals.
Priya Bhardwaj is a Scientist in the Department of Pharmacology at the All India Institute of Medical Sciences (AIIMS), New Delhi. She obtained her B.Sc. (Hons.) in Zoology, M.Sc. in Zoology, and Ph.D. from the University of Delhi, India. During her Masters degree, she specialized in reproductive biology and subsequently received training in clinical research methods in public health.
Her research paper got published in reputed National and International journals and has over five years of experience in conducting reproductive biology practicals for postgraduate students. Her research interests include proteomics-based cancer diagnostics, cardiovascular pharmacology, COVID-19, and reproductive health. Dr. Bhardwaj has also been recognized as a resource person in several workshops, refresher courses, and faculty development programs.
Lisainfo e-raamatute kohta