Lipid Second Messengers provides detailed methodology for analysis of various lipid signaling pathways. Authoritative contributors explain the factors that regulate lipid second messenger production by agonist-activated enzymes and examine their products. Topics discussed include procedures used to measure lipid-derived mediators such as lysophospholipids, arachidonic acid, eicosanoids, anandamide, and ceramides, and the enzymes responsible for generating these messengers, such as phospholipases, prostaglandin endoperoxide synthases, and sphingomyelinase.
Presents an analysis of various lipid signaling pathways. This book presents fresh experimental tools to investigate novel lipid mediators in signal transduction. It examines the role of lipid mediators in signal transduction and cell-to-cell communication.
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...a valuable account of a developing technology that may, at long last, place liposomes on the map of sucessful drug delivery systems." - J. Controlled Release
Preface -
Chapter-1 Phospholipase D /Louis Freysz and JulianN. Kanfer -
Chapter-2 Analyzing the Sphingomyelin Cycle: Protocols for Measuring Sphingomyelinase, Sphingomyelin, and Ceramide /Heather Hayter and Yusuf A. Hannun -
Chapter-3 Expression of the Human Prostaglandin Endoperoxide H Synthases: Measurement of Cyclooxygenase Activity and Inhibition by Nonsteroidal Anti-Inflammatory Drugs /David DeWitt -
Chapter-4 Phospholipid Growth Factors: Identification and Mechanism of Action /Gabor J. 1igyi, Karoly Liliom, David J. Fischer, and Zhong Guo -
Chapter-5 Leukotriene and Lipoxin Biosynthesis /Bruce D. Levy, Karsten Gronert, Clary Clish, and Charles N. Serhan -
Chapter-6 Purification and High-Resolution Analysis of Anandamide and Other Fatty Acylethanolamides /Andrea Giuffrida and Daniele Piomelli -- Index.
Suzanne Laychock, Ph.D., is Professor and Associate Chair of the Department of Pharmacology & Toxicology at the State University of New York at Buffalo, School of Medicine and Biomedical Sciences. She has been studying signal transduction mechanisms involving phospholipases, fatty acids, and eicosanoids since 1975. A major thrust of her work has been in defining the role of phospholipid hydrolysis and turnover, and prostaglandin synthesis in the control of endocrine cells. Her early pioneering work focused on the radioimmunoassay of prostaglandins and the characterization of calcium-dependent phospholipase A2 in adrenocortical cells and isolated pancreatic islets. Her laboratory was also the first to demonstrate calcium regulation of phospholipase C-mediated hydrolysis of phosphoinositides. Dr. Laychock has published over 60 full publications and written three reviews and seven book chapters. She has served as a member of the Editorial Board of several prestigious journals. Ronald P. Rubin, Ph.D., is Professor and Chair of the Department of Pharmacology & Toxicology at the State University of New York at Buffalo, School of Medicine and Biomedical Sciences. Dr. Rubin conducted pioneering work on the pivotal role of calcium in stimulus-response coupling in secretory cells. In addition, he has published widely on the role of lipid mediators in regulating the secretory process in a number of different cell types, including adrenocortical cells, pancreatic and parotid acinar cells, and neutrophils. His current research involves the signal transduction processes associated with the regulation of calcium signaling in parotid acinar cells. Dr. Rubin has published over 100 full publications, written several reviews, and authored two monographs. He also coedited a volume on Calcium in Biological Systems.
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