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E-raamat: Mass Spectrometry-Based Lipidomics: Methods and Protocols

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  • Formaat: EPUB+DRM
  • Sari: Methods in Molecular Biology 2306
  • Ilmumisaeg: 05-May-2021
  • Kirjastus: Springer-Verlag New York Inc.
  • Keel: eng
  • ISBN-13: 9781071614105
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Mass Spectrometry-Based Lipidomics: Methods and ProtocolsSuurem pilt
  • Formaat: EPUB+DRM
  • Sari: Methods in Molecular Biology 2306
  • Ilmumisaeg: 05-May-2021
  • Kirjastus: Springer-Verlag New York Inc.
  • Keel: eng
  • ISBN-13: 9781071614105
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This detailed volume covers conventional MS-based “shotgun lipidomics” by which samples are introduced by infusion or loop injection, as well as LC-MS-based lipidomics, which are becoming increasingly important due to the ever-increasing demand for a complete and precise lipid analysis of the complex and diversified lipids in nature. The volume features protocols applying chemical reactions, the on-line photochemical reactions combined with various MS methods for comprehensive characterization of various lipid classes, and quantification of specific and rare lipids. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Mass Spectrometry-Based Lipidomics: Methods and Protocols serves as an invaluable guide for biochemists and mass spectroscopists who are interested in lipid studies.
Mass Spectrometry-Based Lipidomics: An Overview.- High Confidence
Shotgun Lipidomics Using Structurally Selective Ion Mobility-Mass
Spectrometry.- Global Lipidomics Profiling by a High Resolution LC-MS
Platform.- Comprehensive Structural Characterization of Lipids by Coupling
PaternòBüchi Reaction and Tandem Mass Spectrometry.- Chemical
Derivatization-Aided High Resolution Mass Spectrometry for Shotgun Lipidome
Analysis.- Quantitative Analysis of Polyphosphoinositide,
Bis(monoacylglycero)phosphate, and Phosphatidylglycerol Species by Shotgun
Lipidomics after Methylation.- A Mass Spectrometry-Based Shotgun Lipidomics
Using Charge-Switch Derivatization for Analysis of the Complex Long-Chain
Fatty Acids.- One-Pot Extractive Transesterification of Fatty Acids Followed
by DMOX Derivatization for Location of Double Bonds Using GC-EI-MS.- Ceramide
Analysis by Multiple Linked-Scan Mass Spectrometry Using a Tandem Quadrupole
Instrument.- Comprehensive Mouse Skin Ceramide Analysis on a Solid-Phase and
TLC Separation with High Resolution Mass Spectrometry Platform.- Mass
Spectrometric Analysis of Meibomian Gland Lipids.- Quantification of Plasma
Oxylipins Using Solid Phase Extraction and Reversed-Phase Liquid
Chromatography-Triple Quadrupole Mass Spectrometry.- Targeted Lipidomics of
Drosophila melanogaster During Development.- Shotgun Lipidomic Analysis of
Leishmania Cells.- Characterization of the Uncommon Lipid Families in
Corynebacterium glutamicum by Mass Spectrometry.- Mass Spectrometric Analysis
of Bioactive Sphingolipids in Fungi.- Analytical Methodologies for Lipidomics
in Hemp Plant.- Shotgun Bacterial Lipid A Analysis Using Routine MALDI-TOF
Mass Spectrometry.- Imaging of Polar and Nonpolar Lipids Using Desorption
Electrospray Ionization/Post-Photoionization Mass Spectrometry.-
Matrix-Assisted Laser Desorption/IonizationMass Spectrometry Imaging of
Lipids in the Ischemic Rat Brain Section: A Practical Approach.
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