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E-raamat: Measles and Related Morbilliviruses: Methods and Protocols

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  • Formaat: EPUB+DRM
  • Sari: Methods in Molecular Biology 2808
  • Ilmumisaeg: 14-May-2024
  • Kirjastus: Springer-Verlag New York Inc.
  • Keel: eng
  • ISBN-13: 9781071638705
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  • Formaat: EPUB+DRM
  • Sari: Methods in Molecular Biology 2808
  • Ilmumisaeg: 14-May-2024
  • Kirjastus: Springer-Verlag New York Inc.
  • Keel: eng
  • ISBN-13: 9781071638705

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This volume covers the latest protocols used to study the molecular biology of morbilliviruses, including cell entry, replication, and virus-host interactions. The chapters in this book also cover methods for studying morbillivirus infection through the use of novel tissue culture and in vivo models; analyzing the biological implications of infection by using primary tissues isolated from infected animals; and exploring diagnostic tools to evaluate humoral immunity against human-pathogenic measles virus (MeV). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Measles and Related Morbilliviruses: Methods and Protocols is a valuable resource for researchers studying MeV,and will be useful to researchers studying other members of Paramyxoviridae and Mononegavirales

A Quantitative Fusion Assay to Study Measles Virus Entry.- A
Semi-Quantitative Protein-Fragment Complementation Assay to Study
Protein-Protein Interactions of the Polymerase Complex In Cellula.- Design
and Execution of In Vitro Polymerase Assays for Measles Virus and Related
Mononegaviruses.- Optical Control of Mononegavirus Gene Expression and
Replication.- Generation of Defective Interfering Particles of
Morbilliviruses Using Reverse Genetics.- Molecular Analysis of Copy Back
Defective Interfering RNAs of Morbilliviruses.- Applying Reverse Genetics to
Study Measles Virus Interactions with the Host.- Identification of Host
Factors of Paramyxoviruses by siRNA Genome-Wide Screens.- Dual RNA-seq
Analysis of Patients' Cells and Viral Genome After Measles
Infection.- Investigating Liquid-Liquid Phase Separation in Virus-Generated
Inclusion Bodies Using Fluorescence Recovery After Photobleaching of
Fluorescently Labeled Host Proteins.- Culturing Immortalized Human Airway
Epithelial Cells at an Air-Liquid Interface for Measles Virus Infection.- In
Vitro Modeling of Feline Morbillivirus Infections Using Primary Feline Kidney
Cells.- Setup of Aerosol Delivery System to Prevent Measles Virus Infection
in Nonhuman Primate Model.- Histopathological Analysis of Brains from Dogs
Infected with Canine Distemper Virus.- Canine Distemper Virus Pathogenesis in
the Ferret Model.- Measles Foci Reduction Neutralization Test
(FRNT).- Multiplex Bead Assay for Serological Surveillance of Measles and
Rubella.- Measles IgG Avidity Assay.