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E-raamat: Membrane Lipids: Methods and Protocols

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Membrane Lipids: Methods and ProtocolsSuurem pilt
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This detailed book explores examples of current in vitro and in silico techniques that are at the forefront of lipid membrane research today. Beginning with methods and strategies associated with the creation and use of lipid membrane models in various research settings, the volume continues with electrical impedance spectroscopy strategies and methods to identify how ions and proteins interact with model lipid bilayers, guidance on lipid bilayer in silico molecular dynamics modeling, novel techniques to explore lipid bilayer characteristics using neutron scattering, IR spectroscopy, and atomic force microscopy (AFM), as well as unique fluorescence techniques. Written in the highly successful Methods in Molecular Biology series style, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Membrane Lipids: Methods and Protocols serves as an ideal guide for researchers seeking to further investigate the often complicated world of lipid membrane biophysics.
Methods for Forming Giant Unilamellar Fatty Acid Vesicles.- Preparing
Ion Channel Switch Membrane-Based Biosensors.- Langmuir-Schaefer Deposition
to Create an Asymmetrical Lipopolysaccharide Sparsely-Tethered Lipid
Bilayer.- Electrochemical Impedance Spectroscopy as a Convenient Tool to
Characterize Tethered Bilayer Membranes.- Measuring
Voltage-Current Characteristics of Tethered Bilayer Lipid Membranes to
Determine the Electro-Insertion Properties of Analytes.- Measuring Activation
Energies for Ion Transport Using Tethered Bilayer Lipid Membranes (tBLMs).-
Determining the Pore Size of Multimeric Peptide Ion Channels Using Cation
Conductance Measures of Tethered Bilayer Lipid Membranes.- De-Insertion
Current Analysis of Pore-Forming Peptides and Proteins Using Gold
Electrode-Supported Lipid Bilayer.- Drug Meets Monolayer: Understanding the
Interactions of Sterol Drugs with Models of the Lung Surfactant Monolayer
Using Molecular Dynamics Simulations.- Establishing a Lipid Bilayerfor
Molecular Dynamics Simulations.- Initiating Coarse-Grained MD Simulations for
Membrane-Bound Proteins.- Small Angle Neutron Scattering of Liposomes: Sample
Preparation to Simple Modeling.- Time-Resolved SANS to Measure Monomer
Inter-Bilayer Exchange and Intra-Bilayer Translocation.- Identifying Membrane
Lateral Organization by Contrast-Matched Small Angle Neutron Scattering.-
Using refnx to Model Neutron Reflectometry Data from Phospholipid Bilayers.-
Surface-Enhanced Infrared Absorption Spectroscopy (SEIRAS) to Probe
Interfacial Water in Floating Bilayer Lipid Membranes (fBLMs).- Manipulation
of Lipid Membranes with Thermal Stimuli.- Analyzing Morphological Properties
of Early-Stage Toxic Amyloid Oligomers by Atomic Force Microscopy.-
Formation and Nanoscale Characterization of Asymmetric Supported Lipid
Bilayers Containing Raft-Like Domains.- Rapid FLIM Measurement of Membrane
Tension Probe Flipper-TR.- Bacterial Dye Release Measures in Response to
Antimicrobial Peptides.- Quantitative Measurements of Membrane Lipid Order in
Yeast and Fungi.
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