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E-raamat: Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols: Cutting-Edge Technologies and Advancements

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This third edition volume expands on the previous editions with an update on the latest techniques used for the detection, genotyping, and investigating pathogenesis of Staphylococcus aureus in vitro and in vivo. The methods covered in this book mostly focus on routine clinical diagnosis, surveillance, research, and practice for treatment of patients infected by multi-drug resistant S. aureus. The book also covers the epidemiology of MRSA, molecular typing approaches, clinical treatment of MRSA infections, and animal models of drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Informative and cutting-edge, Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols: Cutting-Edge Technologies and Advancements, Third Edition is a valuable resource for researchers looking to set up new methods to study S. aureus, and will also be very useful for technicians and scientists working on other bacterial pathogens. 

Clinical, Epidemiologic, and Laboratory Aspects of Methicillin-Resistant
Staphylococcus aureus Infections.- Rapid Methods for Detection of MRSA in
Clinical Specimens.- Immunofluorescence Microscopy for the Detection of
Surface Antigens in Methicillin Resistant Staphylococcus
aureus.- Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of
MRSA.- Pulsed-Field Gel Electrophoresis Typing of Staphylococcus aureus
Strains.- Spa Genotyping of Staphylococcus aureus Isolates.-  Multilocus
Sequence Typing of Staphylococcus aureus.- Genetic Manipulations of
Staphylococcal Chromosomal DNA.- Genetic Manipulation of MRSA using
CRISPR/Cas9 Technology.-  RNA-seq Identification of Target Genes Mediated by
Regulators of Staphylococcus aureus.- Application of Two-Dimensional
Difference Gel Electrophoresis in Identification of Factors Responsible for
Virulence of Staphylococcus aureus.- Identification of Virulence Determinants
during Host-Pathogen Interaction using Tn-Seq Technology.- Metabolomic
Profiling of Staphylococcus aureus.- Determining Impact of Growth Phases on
Capacity of Staphylococcus aureus to Adhere to and Invade Host
Cells.- Preclinical Models and Methodologies for Monitoring Staphylococcus
aureus Infections using Noninvasive Optical Imaging.- Methicillin-Resistant
Staphylococcus aureus Infection and Treatment Options.- Animal Models for
Drug Development for MRSA.
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