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E-raamat: Microarray Technology: Methods and Applications

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This volume provides updates of this established field in both methods and applications, as well as advances in applications of the microarray method to biomarkers such as DNAs, RNAs proteins, glycans and whole cells. Written for theMethods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Microarray Technology: Methods and Applications aims to ensure successful results in the further study of this vital field.

Dedication v
Preface vii
Contributors xi
Part I Introduction
1 Overview of Microarray Technology
3(4)
Paul C.H. Li
Part II Advances on Techniques in Microarray Technology
2 Surface Functionalization for Immobilization of Probes on Microarrays
7(18)
C. Desmet
C.A. Marquette
3 The "Clickable" Method for Oligonucleotide Immobilization Onto Azide-Functionalized Microarrays
25(12)
Tomasz Ratajczak
Barbara Uszczynska
Emilia Frydrych-Tomczak
Marcin K. Chmielewski
4 Microarray Developed on Plastic Substrates
37(16)
Maria-Jose Banuls
Sergi B. Morais
Luis A. Tortajada-Genaro
Angel Maquieira
5 Detection and Quantification of MicroRNAs by Ligase-Assisted Sandwich Hybridization on a Microarray
53(14)
Kyo Iizuka
Tare Ueno
Takashi Funatsu
6 Probe Design Strategies for Oligonucleotide Microarrays
67(18)
Nicolas Parisot
Eric Peyretaillade
Eric Dugat-Bony
Jeremie Denonfoux
Antoine Mahul
Pierre Peyret
Part III Applications of Microarray Technology to Nucleic Acid Assays
7 Analyzing Illumina Gene Expression Microarray Data Obtained From Human Whole Blood Cell and Blood Monocyte Samples
85(14)
Alexander Teumer
Claudia Schurmann
Arne Schillert
Katharina Schramm
Andreas Ziegler
Holger Prokisch
8 Microarray Technology and Its Applications for Detecting Plasma microRNA Biomarkers in Digestive Tract Cancers
99(12)
Hirotaka Konishi
Daisuke Ichikawa
Tomohiro Arita
Eigo Otsuji
9 Application of DNA Microarray to Clinical Diagnostics
111(22)
Ankita Patel
Sau W. Cheung
10 High-Throughput DNA Array for SNP Detection of KRAS Gene Using a Centrifugal Microfluidic Device
133(10)
Abootaleb Sedighi
Paul C.H. Li
11 Single Nucleotide Polymorphisms as Genomic Markers for High-Throughput Pharmacogenomic Studies
143(18)
Annalisa Lonetti
Maria Chiara Fontana
Giovanni Martinelli
Ilaria Iacobucci
12 DNA Microarray-Based Diagnostics
161(20)
Mahsa Gharibi Marzancola
Abootaleb Sedighi
Paul C.H. Li
Part IV Applications of Microarray Technology to Other Biological Assays
13 High-Throughput Screening of Substrate Specificity for Protein Tyrosine Phosphatases (PTPs) on Phosphopeptide Microarrays
181(16)
Liqian Gao
Su Seong Lee
Jun Chen
Hongyan Sun
Yuliang Zhao
Zhifang Chai
Yi Hu
14 Nanotechnology in the Fabrication of Protein Microarrays
197(12)
Manuel Fuentes
Paula Diez
Juan Casado-Vela
15 Epitope Mapping Using Peptide Microarray in Autoantibody Profiling
209(16)
Sebastian Henkel
Robert Wellhausen
Dirk Woitalla
Katrin Marcus
Caroline May
16 Preparation of Glycan Arrays Using Pyridylaminated Glycans
225(12)
Shin-Ichi Nakakita
Jun Hirabayashi
17 Competitive Immunoassays Using Antigen Microarrays
237(12)
Zhaowei Zhang
Weihua Hu
Qi Zhang
Peiwu Li
Changming Li
18 Parallel Syntheses of Peptides on Teflon-Patterned Paper Arrays (SyntArrays)
249(24)
Frederique Deiss
Yang Yang
Ratmir Derda
19 Cell Microarrays for Biomedical Applications
273(20)
Mario Rothbauer
Verena Charwat
Peter Ertl
Index 293
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