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E-raamat: Microbial Biofilms: Methods and Protocols

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Microbial Biofilms: Methods and ProtocolsSuurem pilt
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The discovery that most of the chronic infections in humans, including the oral, lung, vaginal and foreign body-associated infections, are biofilm-based, has prompted the need to design new and properly focused preventive and therapeutic strategies for these diseases. Microbial Biofilms: Methods and Protocols provides a detailed description of the currently available methods and protocols to investigate bacterial and fungal biofilms, exhaustively illustrated and critically annotated in 25 chapters written by authors well known for their experience in the respective fields. The book has joined together microbiologists and specialists in infectious diseases, hygiene and public health involved in exploring different aspects of microbial biofilms as well as in designing new methods and/or developing innovative laboratory protocols. Written in the successfulMethods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Microbial Biofilms: Methods and Protocolspresents readers with the most established and validated experimental procedures to investigate microbial biofilms.

Arvustused

From the book reviews:

This book is organized in three parts: Investigations on biofilms in health and disease, Investigations on anti-biofilm compounds and strategies, and Investigations on biofilms in the environment and manufacturing plants. this book should be read by students, researchers, and teachers working in the field of biofilms, as well as scientists working in Microbiology, Ecology or Medicine. (Ioan I. Ardelean, Bulletin of Micro and Nanoelectrotechnologies, Vol. 5 (3-4), December, 2014)

Preface v
Contributors xi
PART I INVESTIGATIONS ON BIOFILMS IN HEALTH AND DISEASE
1 Methods for Dynamic Investigations of Surface-Attached In Vitro Bacterial and Fungal Biofilms
3(20)
Clans Sternberg
Thomas Bjarnsholt
Mark Shirtliff
2 Aqueous Two-Phase System Technology for Patterning Bacterial Communities and Biofilms
23(10)
Mohammed Dwidar
Shuichi Takayama
Robert J. Mitchell
3 Quorum Sensing in Gram-Positive Bacteria: Assay Protocols for Staphylococcal agr and Enterococcal fsr Systems
33(10)
Akane Shojima
Jiro Nakayama
4 Advanced Techniques for In Situ Analysis of the Biofilm Matrix (Structure, Composition, Dynamics) by Means of Laser Scanning Microscopy
43(22)
Thomas R. Neu
John R. Lawrence
5 Multiplex Fluorescence In Situ Hybridization (M-FISH) and Confocal Laser Scanning Microscopy (CLSM) to Analyze Multispecies Oral Biofilms
65(8)
Lamprini Karygianni
Elmar Hellwig
Ali Al-Ahmad
6 Field Emission Scanning Electron Microscopy of Biofilm-Growing Bacteria Involved in Nosocomial Infections
73(12)
Claudia Vuotto
Gianfranco Donelli
7 Experimental Approaches to Investigating the Vaginal Biofilm Microbiome
85(20)
Marc M. Baum
Manjula Gunawardana
Paul Webster
8 Imaging Bacteria and Biofilms on Hardware and Periprosthetic Tissue in Orthopedic Infections
105(22)
Laura Nistico
Luanne Hall-Stoodley
Paul Stoodley
9 Animal Models to Evaluate Bacterial Biofilm Development
127(14)
Kim Thomsen
Hannah Trøstrup
Claus Moser
10 Animal Models to Investigate Fungal Biofilm Formation
141(18)
Jyotsna Chandra
Eric Pearlman
Mahmoud A. Ghannoum
11 Nonmammalian Model Systems to Investigate Fungal Biofilms
159(16)
Marios Arvanitis
Beth Burgwyn Fuchs
Eleftherios Mylonakis
PART II INVESTIGATIONS ON ANTI-BIOFILM COMPOUNDS AND STRATEGIES
12 Microbiological Methods for Target-Oriented Screening of Biofilm Inhibitors
175(12)
Livia Leoni
Paolo Landini
13 In Vitro Screening of Antifungal Compounds Able to Counteract Biofilm Development
187(16)
Marion Girardot
Christine Imbert
14 Biofilm Matrix-Degrading Enzymes
203(12)
Jeffrey B. Kaplan
15 Efficacy Evaluation of Antimicrobial Drug-Releasing Polymer Matrices
215(12)
Iolanda Francolini
Antonella Piozzi
Gianfranco Donelli
16 Antibiotic Polymeric Nanoparticles for Biofilm-Associated Infection Therapy
227(12)
Wean Sin Cheow
Kunn Hadinoto
17 Pharmacokinetics and Pharmacodynamics of Antibiotics in Biofilm Infections of Pseudomonas aeruginosa In Vitro and In Vivo
239(16)
Wang Hengzhuang
Niels Høiby
Oana Ciofu
18 Contribution of Confocal Laser Scanning Microscopy in Deciphering Biofilm Tridimensional Structure and Reactivity
255(12)
Arnaud Bridier
Romain Briandet
19 Chip Calorimetry for Evaluation of Biofilm Treatment with Biocides, Antibiotics, and Biological Agents
267(10)
Frida Mariana Morais
Friederike Buchholz
Thomas Maskow
20 Bacteriophage Attack as an Anti-biofilm Strategy
277(10)
Sanna Sillankorva
Joana Azeredo
21 Photodynamic Therapy as a Novel Antimicrobial Strategy Against Biofilm-Based Nosocomial Infections: Study Protocols
287(14)
Francesco Giuliani
PART III INVESTIGATIONS ON BIOFILMS IN THE ENVIRONMENT AND MANUFACTURING PLANTS
22 Capturing Air--Water Interface Biofilms for Microscopy and Molecular Analysis
301(22)
Margaret C. Henk
23 Biofilm-Growing Bacteria Involved in the Corrosion of Concrete Wastewater Pipes: Protocols for Comparative Metagenomic Analyses
323(18)
Vicente Gomez-Alvarez
24 Culture-Independent Methods to Study Subaerial Biofilm Growing on Biodeteriorated Surfaces of Stone Cultural Heritage and Frescoes
341(26)
Francesco
Cappitelli
Federica Villa
Andrea Polo
25 Biofilms of Thermophilic Bacilli Isolated from Dairy Processing Plants and Efficacy of Sanitizers
367(12)
Sara A. Burgess
Denise Lindsay
Steve H. Flint
Index 379
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