Klienditugi: 7440010 (E-R 10-18)

E-raamat: Microbial Biotechnology

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David Lea-Smith (University of East Anglia), Beatrix Fahnert (University of Lincoln), Tom Clarke (University of East Anglia), Kay Yeoman (University of East Anglia)

Formaat: 224 pages
Sari: Oxford Biology Primers
Ilmumisaeg: 01-Dec-2020
Kirjastus: Oxford University Press
Keel: eng
ISBN-13: 9780192555571

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Formaat: 224 pages
Sari: Oxford Biology Primers
Ilmumisaeg: 01-Dec-2020
Kirjastus: Oxford University Press
Keel: eng
ISBN-13: 9780192555571

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Written primarily for students embarking on an undergraduate bioscience degree, this primer will introduce students to topics at the forefront of the subject that are being applied to probe biological problems, or to address the most pressing issues facing society. These topics will include those that form the cornerstone of contemporary research, helping students to make the transition to active researcher.

Students will acquire a solid understanding of the essentials of microbial biotechnology, its applications in agriculture, diagnostics and urban and artistic conservation, as well as the potential threats genetic modification may pose to public health, the environment and intellectual property.

1 The basic principles of a biotechnology process

(27)

1.1 Overview of the biotechnology process

(2)

1.2 The hosts

(5)

1.3 Genetic manipulation

(1)

1.4 Metabolic engineering

(2)

1.5 Heterologous protein expression

(1)

1.6 Fermentation media and media design

(5)

1.7 Types of reactor

(5)

1.8 Scale-up and optimization

(2)

1.9 Downstream processing

(3)

2 Microbial Growth

(24)

2.1 Measurement of microbial growth

(3)

2.2 Microbial growth in different culture systems

(2)

2.3 Microbial growth phases in batch culture

(2)

2.4 Cell division in the exponential growth phase

(2)

2.5 Microbial growth rates

(1)

2.6 Modelling microbial growth in batch culture

(6)

2.7 Continuous culture

(3)

2.8 Fed-batch culture

(4)

3 Microbial bio-production

(1)

3.1 Pharmaceutical products

(16)

3.2 Fine chemicals

(2)

3.3 Polymers

(3)

3.4 Biofuels

(4)

3.5 Bioelectrogenesis

(4)

4 Biotechnology and food and drink production

(1)

4.1 Microbes producing components for food

(6)

4.2 Microbes used to make food

(9)

4.3 Microbes used to make alcoholic beverages

(5)

4.4 Microbes as food: single-cell protein

103

(6)

4.5 Microbes producing food supplements

109

(7)

5 Environmental biotechnology

116

(16)

5.1 Wastewater treatment

117

(2)

5.2 Anaerobic digestion of solid waste

119

(2)

5.3 Hydrocarbon bioremediation

121

(3)

5.4 Recovery of metals and bioleaching

124

(2)

5.5 Environmental monitoring

126

(2)

5.6 Gas bioscrubbing

128

(1)

5.7 Bioaugmentation

129

(3)

6 Application of synthetic biology to biotechnology

132

(18)

6.1 Microorganisms widely used in synthetic biology

133

(1)

6.2 Metabolic pathway modelling

134

(2)

6.3 Gene synthesis

136

(1)

6.4 Using biological parts to facilitate genetic manipulation of microbes

137

(3)

6.5 Directed evolution of cells

140

(1)

6.6 Modular protein assembly

141

(1)

6.7 Compartmentalization of metabolic processes

142

(2)

6.8 Synthetic cells

144

(1)

6.9 Translation of synthetic biology to commercial applications

145

(5)

7 Diagnostics

150

(23)

7.1 Principles of diagnostics

151

(4)

7.2 Mechanisms of detection

155

(6)

7.3 Biosensors and their applications

161

(9)

7.4 Detection of microbial cells

170

(3)

8 Microbial biotechnology and agriculture

173

(25)

8.1 The soil environment

174

(3)

8.2 Microbial inoculants

177

(11)

8.3 Microbial biopesticides

188

(4)

8.4 Microbes as tools for genetic modification

192

(6)

9 Using extremophiles in biotechnology

198

(20)

9.1 Extreme temperature

200

(6)

9.2 Extreme pressure

206

(1)

9.3 Extreme pH

207

(4)

9.4 Extreme osmotic pressure

211

(7)

10 Microbial biotechnology in the art and built environment

218

(20)

10.1 Biodeterioration

219

(9)

10.2 Countering biodeterioration

228

(4)

10.3 Creating and creativity

232

(6)

11 Ethical considerations

238

(11)

11.1 Some contexts for ethical consideration

240

(5)

11.2 Some unethical contexts

245

(4)

Glossary

249

(6)

Bibliography

255

(4)

Index

259

Professor Kay Yeoman teaches microbiology in the School of Biological Sciences at UEA. She has undertaken research in the use of agricultural waste materials in fermentation media. She has also conducted research in the Rhizobium:legume symbiosis, investigating the uptake of iron. She is keen communicator of science, leads fungal forays and is particularly interested in fungi and their use in industry.

Dr Beatrix Fahnert is a microbiologist with a background in medical, industrial and applied microbiology. She has been teaching related subjects at Undergraduate and Postgraduate level for more than 20 years, and is currently an Associate Professor at the School of Life Sciences, University of Lincoln. Beatrix is a member of the European Federation of Biotechnology and the UK Microbiology Society, where she served as Chair-Elect of the Education Division.

Dr David Lea-Smith is a lecturer in microbiology at the University of East Anglia. His research is primarily focused on investigating photosynthetic cyanobacteria, specifically understanding their biochemistry and physiology, impact on the environment and potential for biotechnology as a platform for renewable production of industrial and medical compounds.

Dr Tom Clarke graduated from UCL in Biochemistry and undertook a PhD at the John Innes centre in Norwich. After a research fellowship at the University of Michigan he joined UEA biological sciences in 2003. His current research involves uncovering how bacteria generate electricity in different environments.

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