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E-raamat: Microfluidic Devices for Biomedical Applications

Edited by (Department of R&D at ABS Global Inc., USA), Edited by (Department of Chemistry and Biochemistry, Biomedical Engineering, Environmental Science and Engineering, Border Biomedical Research Center, University of Texas at El Paso)
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Mechanical and biomedical engineers, chemists, and other contributors briefly introduce the fundamentals of microfluidics, then survey current research into microfluidic devices or lab-on-chip platforms in biomedical applications. Among the topics are: surface coatings for microfluidic-based biomedical devices, actuation mechanisms, controlling drug delivery, drug discovery and analysis, cell manipulation, developing tissue scaffolds, immunoassays for protein analysis of nano-bio-array chips, integrated microfluidic systems for genetic analysis, low-cost assays in paper-based microfluidic biomedical devices, detecting viruses, and radio chemical synthesis. Annotation ©2013 Book News, Inc., Portland, OR (booknews.com)

Microfluidics or lab-on-a-chip (LOC) is an important technology suitable for numerous applications from drug delivery to tissue engineering. Microfluidic devices for biomedical applications discusses the fundamentals of microfluidics and explores in detail a wide range of medical applications.

The first part of the book reviews the fundamentals of microfluidic technologies for biomedical applications with chapters focussing on the materials and methods for microfabrication, microfluidic actuation mechanisms and digital microfluidic technologies. Chapters in part two examine applications in drug discovery and controlled-delivery including micro needles. Part three considers applications of microfluidic devices in cellular analysis and manipulation, tissue engineering and their role in developing tissue scaffolds and stem cell engineering. The final part of the book covers the applications of microfluidic devices in diagnostic sensing, including genetic analysis, low-cost bioassays, viral detection, and radio chemical synthesis.

Microfluidic devices for biomedical applications is an essential reference for medical device manufacturers, scientists and researchers concerned with microfluidics in the field of biomedical applications and life-science industries.
  • discusses the fundamentals of microfluidics or lab-on-a-chip (LOC) and explores in detail a wide range of medical applications
  • considers materials and methods for microfabrication, microfluidic actuation mechanisms and digital microfluidic technologies
  • considers applications of microfluidic devices in cellular analysis and manipulation, tissue engineering and their role in developing tissue scaffolds and stem cell engineering


Microfluidic devices are suitable for numerous applications, from drug delivery to tissue engineering. As microfluidic devices can be cheaply made and are small, applications in the medical fields are continually being researched and produced. This important book discusses the fundamentals of microfluidics and provides information on a wide range of medical applications.

The first part reviews the fundamentals of microfluidic technologies for biomedical applications with chapters focusing on materials and methods for microfabrication, microfluidic actuation mechanisms and digital microfluidic technologies. Chapters in part two examine applications in drug discovery and controlled-delivery including micro needles while the third section considers applications of microfluidic devices in cellular analysis and manipulation, tissue engineering and their role in developing tissue scaffolds and stem cell engineering. The conclusion covers the applications of microfluidic devices in diagnostic sensing, including genetic analysis, low-cost bioassays, viral detection, and radio chemical synthesis.

Arvustused

"Mechanical and biomedical engineers, chemists, and other contributors briefly introduce the fundamentals of microfluidics, then survey current research into microfluidic devices or lab-on-chip platforms in biomedical applications. Among the topics are: surface coatings for microfluidic-based biomedical devices, actuation mechanisms," --ProtoView.com, March 2014

Contributor contact details xii
Woodhead Publishing Series in Biomaterials xvi
About the editors xx
Preface xxi
Part I Fundamentals of microfluidic technologies for biomedical applications
1(164)
1 Materials and methods for the microfabrication of microfluidic biomedical devices
3(60)
W. I. Wu
P. Rezai
H. H. Hsu
P. R. Selvaganapathy
1.1 Introduction
3(1)
1.2 Microfabrication methods
4(6)
1.3 Materials for biomedical devices
10(9)
1.4 Polymers
19(24)
1.5 Conclusion and future trends
43(1)
1.6 References
44(18)
1.7 Appendix: acronyms
62(1)
2 Surface coatings for microfluidic-based biomedical devices
63(37)
B. G. Abdallah
A. Ros
2.1 Introduction
63(2)
2.2 Covalent immobilization strategies: polymer devices
65(8)
2.3 Covalent immobilization strategies: glass devices
73(3)
2.4 Adsorption strategies
76(6)
2.5 Other strategies utilizing surface treatments
82(2)
2.6 Examples of applications
84(6)
2.7 Conclusion and future trends
90(1)
2.8 Sources of further information and advice
91(1)
2.9 References
92(8)
3 Actuation mechanisms for microfluidic biomedical devices
100(39)
A. Rezk
J. Friend
L. Yeo
3.1 Introduction
100(1)
3.2 Electrokinetics
101(17)
3.3 Acoustics
118(10)
3.4 Limitations and future trends
128(2)
3.5 References
130(9)
4 Digital microfluidics technologies for biomedical devices
139(26)
C. M. Collier
J. Nichols
J. F. Holzman
4.1 Introduction
139(3)
4.2 On-chip microdrop motion techniques
142(13)
4.3 Sensing techniques
155(6)
4.4 Future trends
161(1)
4.5 Conclusion
161(1)
4.6 References
162(3)
Part II Applications of microfluidic devices for drug delivery and discovery
165(116)
5 Controlled drug delivery using microfluidic devices
167(18)
N. Gao
5.1 Introduction
167(2)
5.2 Microreservoir-based drug delivery systems
169(6)
5.3 Micro/nanofluidics-based drug delivery systems
175(6)
5.4 Conclusion
181(1)
5.5 Future trends
182(1)
5.6 References
182(3)
6 Microneedles for drug delivery and monitoring
185(46)
T. R. R. Singh
H. McMillan
K. Mooney
A. Z. Alkilani
R. F. Donnelly
6.1 Introduction
185(2)
6.2 Fabrication of microneedles (MNs)
187(3)
6.3 MN design parameters and structure
190(6)
6.4 Strategies for MN-based drug delivery
196(6)
6.5 MN-mediated monitoring using skin interstitial fluid (ISF) and blood samples
202(11)
6.6 Future trends
213(5)
6.7 Conclusion
218(1)
6.8 References
219(12)
7 Microfluidic devices for drug discovery and analysis
231(50)
J. S. Kochhar
S. Y. Chan
P. S. Ong
W. G. Lee
L. Kang
7.1 Introduction
231(2)
7.2 Microfluidics for drug discovery
233(24)
7.3 Microfluidics for drug analysis and diagnostic applications
257(11)
7.4 Conclusion and future trends
268(1)
7.5 Sources of further information and advice
269(1)
7.6 References
269(12)
Part III Applications of microfluidic devices for cellular analysis and tissue engineering
281(162)
8 Microfluidic devices for cell manipulation
283(68)
H. O. Fatoyinbo
8.1 Introduction
283(2)
8.2 Microenvironment on cell integrity
285(2)
8.3 Microscale fluid dynamics
287(6)
8.4 Manipulation technologies
293(36)
8.5 Manipulation of cancer cells in microfluidic systems
329(5)
8.6 Conclusion and future trends
334(1)
8.7 Sources of further information and advice
334(1)
8.8 References
335(16)
9 Microfluidic devices for single-cell trapping and automated micro-robotic injection
351(12)
X. Y. Liu
Y. Sun
9.1 Introduction
351(2)
9.2 Device design and microfabrication
353(2)
9.3 Experimental results and discussion
355(5)
9.4 Conclusion
360(1)
9.5 Acknowledgements
361(1)
9.6 References
361(2)
10 Microfluidic devices for developing tissue scaffolds
363(25)
L. T. Chau
J. E. Frith
R. J. Mills
D. J. Menzies
D. M. Titmarsh
J. J. Cooper-White
10.1 Introduction
363(1)
10.2 Key issues and technical challenges for successful tissue engineering
364(6)
10.3 Microfluidic device platforms
370(9)
10.4 Conclusion and future trends
379(2)
10.5 References
381(7)
11 Microfluidic devices for stem cell analysis
388(55)
D.-K. Kang
J. Lu
W. Zhang
E. Chang
M. A. Eckert
M. M. Ali
W. Zhao
11.1 Introduction
388(4)
11.2 Technologies used in stem cell analysis
392(10)
11.3 Examples of microfluidic platform for stem cell analysis: stem cell culture platform -- mimicking in vivo culture conditions in vitro
402(8)
11.4 Examples of microfluidic platform for stem cell analysis: single stem cell analysis
410(4)
11.5 Microdevices for label-free and non-invasive monitoring of stem cell differentiation
414(6)
11.6 Microfluidics stem cell separation technology
420(8)
11.7 Conclusion and future trends
428(3)
11.8 Sources of further information and advice
431(1)
11.9 References
431(12)
Part IV Applications of microfluidic devices in diagnostic sensing
443(191)
12 Development of immunoassays for protein analysis on nanobioarray chips
445(20)
J. Lee
P. C. H. Li
12.1 Introduction
445(2)
12.2 Technologies
447(4)
12.3 Immobilization chemistry
451(1)
12.4 Detection methods
452(2)
12.5 Applications
454(8)
12.6 Conclusion and future trends
462(1)
12.7 References
462(3)
13 Integrated microfluidic systems for genetic analysis
465(27)
B. Zhuang
W. Gan
P. Liu
13.1 Introduction
465(2)
13.2 Integrated microfluidic systems
467(1)
13.3 Development of integrated microdevices
468(2)
13.4 Applications of fully integrated systems in genetic analysis
470(12)
13.5 Conclusion and future trends
482(1)
13.6 References
483(9)
14 Low-cost assays in paper-based microfluidic biomedical devices
492(35)
M. Benhabib
San Francisco
14.1 Introduction
492(1)
14.2 Fabrication techniques for paper-based microfluidic devices
493(13)
14.3 Detection and read-out technologies
506(7)
14.4 Application of paper-based microfluidic devices
513(8)
14.5 Conclusion and future trends
521(1)
14.6 References
522(5)
15 Microfluidic devices for viral detection
527(30)
J. Sun
X. Jiang
15.1 Introduction
527(2)
15.2 Microfluidic technologies used for viral detection
529(15)
15.3 Examples of applications
544(6)
15.4 Conclusion and future trends
550(1)
15.5 Acknowledgements
551(1)
15.6 References
551(6)
16 Microfluidics for monitoring and imaging pancreatic islet and β-cells for human transplant
557(37)
Y. Wang
J. E. Mendoza-Elias
F. Feng
Z. Li
Q. Wang
M. Nourmohammadzadeh
D. Gutierrez
M. Qi
D. T. Eddington
J. Oberholzer
16.1 Introduction
557(3)
16.2 Insulin secretory pathway: how glucose sensing and metabolic coupling translates to insulin kinetics
560(2)
16.3 Technologies: the emergence of microfluidics applied to islet and β-cell study
562(3)
16.4 Design and fabrication of the University of Illinois at Chicago (UIC) microfluidic device
565(4)
16.5 Protocol: materials
569(4)
16.6 Protocol: procedures
573(12)
16.7 Anticipated results
585(4)
16.8 Acknowledgements
589(1)
16.9 References
589(5)
17 Microfluidic devices for radio chemical synthesis
594(40)
A. Y. Lebedev
17.1 Introduction
594(1)
17.2 Medical applications of microfluidic radiochemistry: positron emission tomography (PET) and single photon emission computed tomography (SPECT)
595(2)
17.3 Advantages and disadvantages of microfluidic devices
597(4)
17.4 Realization of promises: the superiority of microfluidic systems
601(20)
17.5 Current problems for microfluidic technology
621(5)
17.6 Recent developments with potential impact
626(3)
17.7 Conclusion
629(1)
17.8 References
629(5)
Index 634
XiuJun (James) Li, Ph.D., is an Associate Professor with early tenure in the Department of Chemistry and Biochemistry, Biomedical Engineering, and Border Biomedical Research Center at the University of Texas at El Paso (UTEP), USA. After he obtained his Ph.D. degree in microfluidic lab-on-a-chip bioanalysis from Simon Fraser University (SFU) in Canada in 2008, he pursued his postdoctoral research with Prof. Richard Mathies at University of California Berkeley and Prof. George Whitesides at Harvard University, while holding a Postdoctoral Fellowship from Natural Sciences and Engineering Research Council (NSERC) of Canada. He has gained extensive experience in bioanalysis using microfluidic systems, such as single-cell analysis, genetic analysis, low-cost diagnosis, pathogen detection, 3D cell culture, and so on. Dr. Lis current research interest is centered on the development of innovative microfluidic lab-on-a-chip and nanotechnology for bioanalysis, biomaterial, biomedical engineering, and environmental applications, including but not limited to low-cost diagnosis, nano-biosensing, tissue engineering, and single-cell analysis. He has coauthored about 100 publications in high-impact journals (such as Adv. Drug Deliv. Rev, Appl. Catal. B-Environ, Anal. Chem., Lab Chip, Biosens. Bioelectron.) and 22 patents, including two books from Elsevier on microfluidic devices for biomedical applications. He is an Advisory Board member of Lab on a Chip and Analyst, the Founder of microBioChip Diagnostics LLC, and an editor of 6 journals including Scientific Reports from the Nature publishing group, Micromachines, etc. He is the recipient of the Bioanalysis New Investigator Award” in 2014, UT STARS Award in 2012, NSERC Postdoctoral Fellow Award in 2009, and so on. For more information, please visit http://li.utep.edu. Yu Zhou, PhD, is a Research Scientist in the Department of Research and Development at ABS Global Inc., USA. Dr Zhou received his Ph.D. degree in mechanical engineering from University of Illinois at Chicago in 2010. After graduation, he joined ABS Global, the world-leading genetics provider company as a key researcher and has been working on the development of a high-throughput microfluidic cytometry for biological cell detection and manipulation. He obtained extensive experience in design and fabrication of silicon-based microsystems and disposal plastic microfluidic chips, precision fluid delivery, and microfluidics-based single cell separation and analysis. He is a member of ASME and serves on the advisory editorial board for several technical journals including Microsystem Technologies, and Journal of Mechanical Engineering Research (Canada) since 2011.