| Preface |
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v | |
| Contributors |
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xix | |
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1 Preparation of "Functional" Mitochondria: A Challenging Business |
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1 | (8) |
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2 Isolation and Quality Control of Functional Mitochondria |
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9 | (16) |
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3 Isolation of Mitochondria-Associated ER Membranes (MAMs) and Glycosphingolipid-Enriched Microdomains (GEMs) from Brain Tissues and Neuronal Cells |
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25 | (10) |
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4 Fluorescence Analysis of Single Mitochondria with Nanofluidic Channels |
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35 | (12) |
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5 Optical Microwell Arrays for Large-Scale Studies of Single Mitochondria Metabolic Responses |
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47 | (12) |
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6 Deep Resequencing of Mitochondrial DNA |
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59 | (8) |
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7 Single-Cell Analysis of Mitochondrial DNA |
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67 | (10) |
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8 A High-Throughput Next-Generation Sequencing Assay for the Mitochondrial Genome |
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77 | (12) |
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9 Rapid Mitochondrial DNA Isolation Method for Direct Sequencing |
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89 | (8) |
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10 Analysis of Mitochondrial DNA and RNA Integrity by a Real-Time qPCR-Based Method |
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97 | (10) |
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11 Mitochondria-Targeted RNA Import |
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107 | (10) |
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12 Accurate Measurement of Circulating Mitochondrial DNA Content from Human Blood Samples Using Real-Time Quantitative PCR |
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117 | (16) |
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13 mTRIP: An Imaging Tool to Investigate Mitochondrial DNA Dynamics in Physiology and Disease at the Single-Cell Resolution |
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133 | (16) |
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14 Simultaneous Quantification of Mitochondrial ATP and ROS Production |
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149 | (12) |
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15 Live-Cell Assessment of Mitochondrial Reactive Oxygen Species Using Dihydroethidine |
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161 | (10) |
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16 Detection and Differentiation Between Peroxynitrite and Hydroperoxides Using Mitochondria-Targeted Arylboronic Acid |
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171 | (12) |
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17 Time-Resolved Spectrometry of Mitochondrial NAD(P)H Fluorescence and Its Applications for Evaluating the Oxidative State in Living Cells |
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183 | (12) |
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Alzbeta Marcek Chorvatova |
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18 Novel Methods for Measuring the Mitochondrial Membrane Potential |
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195 | (8) |
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19 High-Throughput Real-Time Analysis of Cell Oxygenation Using Intracellular Oxygen-Sensitive Probes |
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203 | (16) |
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20 In Vivo Assessment of Mitochondrial Oxygen Consumption |
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219 | (12) |
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21 Imaging Mitochondrial Hydrogen Peroxide in Living Cells |
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231 | (14) |
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22 Simultaneous High-Resolution Measurement of Mitochondrial Respiration and Hydrogen Peroxide Production |
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245 | (18) |
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23 Measurement of Mitochondrial NADH and FAD Autofluorescence in Live Cells |
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263 | (8) |
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24 Mitochondrial Coenzyme Q10 Determination via Isotope Dilution Liquid Chromatography Tandem Mass Spectrometry |
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271 | (8) |
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25 Assessing the Bioenergetic Profile of Human Pluripotent Stem Cells |
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279 | (10) |
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26 Integrative Methods for Studying Cardiac Energetics |
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289 | (16) |
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27 Computer-Based Prediction of Mitochondria-Targeting Peptides |
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305 | (16) |
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28 Prediction of Mitochondrial Protein Function by Comparative Physiology and Phylogenetic Profiling |
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321 | (10) |
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29 Assessment of Posttranslational Modification of Mitochondrial Proteins |
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331 | (12) |
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30 Assessment of Mitochondrial Protein Glutathionylation as Signaling for CO Pathway |
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343 | (8) |
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31 High-Resolution Melting Analysis for Identifying Sequence Variations in Nuclear Genes for Assembly Factors and Structural Subunits of Cytochrome C Oxidase |
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351 | (18) |
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32 Heterologous Inferential Analysis (HIA) as a Method to Understand the Role of Mitochondrial rRNA Mutations in Pathogenesis |
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369 | (16) |
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33 Analysis of Mitochondrial Dysfunction During Cell Death |
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385 | (10) |
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34 The Use of FLIM-FRET for the Detection of Mitochondria-Associated Protein Interactions |
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395 | (26) |
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35 Assessment of Mitochondrial Ca2+ Uptake |
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421 | (20) |
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36 Qualitative Characterization of the Rat Liver Mitochondrial Lipidome Using All Ion Fragmentation on an Exactive Benchtop Orbitrap MS |
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441 | (12) |
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37 Characterization of Mitochondrial Populations During Stem Cell Differentiation |
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453 | (12) |
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38 An Ex Vivo Model for Studying Mitochondrial Trafficking in Neurons |
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465 | (8) |
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| Index |
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473 | |