This volume provides readers with a detailed collection of in vitro and in vivo protocols developed to study inflammation and the interactions between host and micro-organisms. The chapters in this book cover a range of biological systems and methodological approaches such as direct inoculation of pathogenic yeasts into mouse lung by trans-tracheal instillation; murine models of experimental periodontal disease; quantitative in vitro and in vivo assays to assess the efferocytic efficiency of macrophages; murine models of alphavirus infection to study the immunopathogenesis of pain; and in vivo preclinical platforms to study coronavirus infection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Models in Inflammation and Immunopharmacology is a practical and conceptual resource for researchers studying inflammation and host-microorganism interactions. This book is also a valuable reference for educators, clinicians, and policymakers who want to understand the foundations of experimental design in biomedicine.
Handling and Experimentation with Germ-Free Mice.- Modeling Gut Barrier
Dysfunction and Subsequent Opportunistic Infection by Candida albicans during
Chemotherapy-Induced Intestinal Mucositis in Mice.- Direct Inoculation of
Pathogenic Yeasts into Mouse Lung by Trans-Tracheal Instillation.- Murine
Models of Experimental Periodontal Disease.- Infectious Models in Mice for
Study of Gut Inflammation: Primary Cultures of Myenteric Neurons.- Intravital
Microscopy of Mesenteric and Brain vessels: A Valuable Tool for Leukocyte
Recruitment Evaluation.- Classical Histological Procedures with Murine
Tissues.- Measurement of MPO, NAG, and EPO as an Indirect Quantification of
Leukocyte Infiltration in Mouse Tissues.- Bridging Immunity and Hemostasis:
Technical Foundations of Platelet-Leukocyte Co-Cultures.- Quantitative In
Vitro and In Vivo Assays to Assess the Efferocytic Efficiency of
Macrophages.- Physical Exercise as a Model for Investigation of the
Inflammation Response in Mice.- Humanized Murine Model with Peripheral Blood
Mononuclear Cells as a Strategy to Translate Experimental Biomedical
Research.- Murine Models of Alphavirus Infection to Study the
Immunopathogenesis of Pain.- Platforms to Evaluate Flavivirus-Induced
Immunoinflammatory Responses and Their Pathogenesis.- Methods for Evaluating
Neurotropic Viral Infections: Exploring Zika and Coronavirus Diseases.- In
Vivo Preclinical Platforms to Study Coronavirus Infection.
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