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E-raamat: Multi-modality Microscopy

Edited by (Nat'l Sun Yat-sen Univ, Taiwan), Edited by (Nus & A*star, S'pore), Edited by (The State Univ Of New York At Buffalo, Usa), Edited by (Nus, S'pore)
  • Formaat: 316 pages
  • Ilmumisaeg: 08-May-2006
  • Kirjastus: World Scientific Publishing Co Pte Ltd
  • Keel: eng
  • ISBN-13: 9789814479127
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Multi-modality MicroscopySuurem pilt
  • Formaat: 316 pages
  • Ilmumisaeg: 08-May-2006
  • Kirjastus: World Scientific Publishing Co Pte Ltd
  • Keel: eng
  • ISBN-13: 9789814479127
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This book covers important aspects of modern optical microscopy and image restoration technologies. Instead of pure optical treatment, the book is delivered with the consideration of the scientists who utilize optical microscopy in their daily research. However, enough details are provided in basic imaging principles, optics and instrumentation in microscopy, spherical aberrations, deconvolution and image restoration. A number of microscopic technologies such as polarization, confocal and multi-photon microscopy are highlighted with their applications in biological and materials sciences/engineering.
Preface
Chapter
1. Basic Principles of Imaging		 1(74)
T. Holmes and P.C. Cheng
1. Light Microscopy
1(7)
2. Image Acquisition
8(6)
3. Data Correction
14(5)
4. Video Enhanced Contrast
19(6)
5. Image Processing
25(15)
6. Deconvolution
40(23)
7. Three-Dimensional Rendering
63(7)
References
70(5)
Chapter
2. Optics for the Biologist: What You Need to Know to Make Your Light Microscope Work Properly		 75(30)
J. Pawley
1. Introduction
75(1)
2. The Problem of Spheres
76(4)
3. 'Tube' Length and Magnification
80(9)
4. What to Do?
89(1)
5. Intermediate Summary
90(1)
6. Detecting Spherical Aberration
90(3)
7. How Do You Correct Spherical Aberration?
93(7)
8. Upon Reflection
100(4)
9. Conclusion
104(1)
References
104(1)
Chapter
3. Spherical Aberration in Confocal Microscopy		 105(20)
C.R. Sheppard
1. Introduction
105(5)
2. Aberration Introduced by Refractive Index Mismatch
110(4)
3. Compensation by Alteration of Immersion Medium Refractive Index
114(3)
4. Validity of the Debye Approximation in the Presence of Spherical Aberration
117(5)
5. Conclusion
122(1)
6. Acknowledgements
122(1)
References
123(2)
Chapter
4. Deconvolution and Image Quality Control Valuable Tools in Multi-Dimensional Light Microscopy		 125(25)
L. Schaefer and A. Kriete
1. Introduction
126(1)
2. Background
127(3)
3. Image Restoration
130(8)
4. Image Quality Control
138(7)
5. Conclusion
145(1)
6. Acknowledgements
146(1)
References
147(3)
Chapter
5. Quantitative Polarised Light Microscopy		 150(12)
T. Wilson, F. Massoumian, R. Juskaitis and M.A.A. Neil
1. Introduction
150(1)
2. Design Outline
151(3)
3. The Experimental System
154(1)
4. Examples of Images
155(5)
5. Conclusion
160(1)
6. Acknowledgements
161(1)
References
161(1)
Chapter
6. Multi-Photon Scanning Microscopy Using a Femtosecond Cr:forsterite Laser		 162(16)
C.-K. Sun, S.-W. Chu, T.-M. Liu and Y-H. Cheng
1. Introduction
162(2)
2. Materials and Methods
164(4)
3. Results and Discussion
168(7)
4. Summary
175(1)
5. Acknowledgements
175(1)
References
176(2)
Chapter
7. Design and Performance Evaluation of a Multi-Functional Microscopy		 178(10)
W.-J. Chen, C.-K. Lee and S.-S. Lu
1. Introduction
178(1)
2. Theory
179(6)
3. Experimental Results
185(2)
4. Conclusions
187(1)
References
187(1)
Chapter
8. Zeeman Laser Scanning Confocal Microscopy in Turbid Media		 188(9)
C. Chou, L.C. Peng, C. W Lyu and J-C. Hsieh
1. Introduction
188(2)
2. Experimental Setup
190(5)
3. Conclusion
195(1)
4. Acknowledgements
195(1)
References
196(1)
Chapter
9. A Method for Real-Time Confocal Imaging of Substrate Surfaces During Active Air Abrasion Cutting: The Cutting Edge of Air Abrasion		 197(22)
R.J Cook, A. Azzopardi, I.D. Thompson and T.F. Watson
1. Introduction
197(4)
2. Materials and Methods
201(4)
3. Results
205(7)
4. Discussion
212(3)
5. Summary
215(1)
6. Acknowledgements
216(1)
References
216(3)
Chapter
10. Three-Dimensional Optical Data Storage with a Multi-layered Recording Medium		 219(13)
Y. Kawata
1. Introduction
219(2)
2. Bit-Oriented 3-D Memory
221(2)
3. Multi-Structured Recording Medium for Reading with Reflection Confocal Microscope
223(5)
4. Two-Photon Recording in Multi-Structured Medium
228(1)
5. Discussion and Conclusion
229(1)
References
230(2)
Chapter
11. The Studies of Single Luminescent Conjugated Polymers		 232(6)
S.-C. Yang, J.D. White, J.-H. Hsu and W. Fann
1. Introduction
232(1)
2. Experimental
233(1)
3. Results and Discussion
234(2)
4. Conclusion
236(1)
References
237(1)
Chapter
12. Confocal Microscopy of GFP-Like Pigments in Corals		 238(8)
G. Cox and A. Salih
1. Introduction
238(1)
2. Materials and Methods
239(1)
3. Results and Discussion
240(3)
4. Conclusion
243(1)
5. Acknowledgements
244(1)
References
244(2)
Chapter
13. Three-Dimensional Localisation of Fluorescence Resonance Energy Transfer in Living Cells under Two-Photon Excitation		 246(11)
M. Gu
1. Introduction
246(2)
2. Experimental Results
248(8)
3. Conclusion
256(1)
4. Acknowledgements
256(1)
References
256(1)
Chapter
14. Multi-Dimensional Imaging of Cell- and Tissue-Engineered Constructs		 257(32)
Y. Zhu, S.S. Ng, Y.M. Khong, L.I. He, Y.C. Toh, X.T. Pan, S. Chia, P.C. Lin, W.X. Sun and H. Yu
1. Introduction
257(2)
2. Natural Spheroids or Engineered Cell Aggregates
259(6)
3. Micro-capsules
265(2)
4. Porous Scaffold-based 3-D Tissue Constructs
267(4)
5. Micro-Fluidic Channels
271(3)
6. Three-Dimensional Tissue Slices
274(5)
7. Conclusion and Future Work
279(1)
References
280(9)
Appendix A. Two-Photon Excited Fluorescent Spectra of Common Biological Dyes 289(2)
Appendix B. Color Figures 291(8)
Index 299


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