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E-raamat: Multiplex Biomarker Techniques: Methods and Applications for COVID-19 Disease Diagnosis and Risk Stratification

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This detailed volume explores the application of multiplex biomarker methods in the critical area of COVID-19 research through state-of-the-art technologies in the fields of genomics, proteomics, transcriptomics, metabolomics, and imaging. The book features a series of protocols from labs across the globe employing multiplex molecular approaches, which can be applied to accelerate progress in the research of SARS-CoV-2 and other infectious illnesses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and essential, Multiplex Biomarker Techniques: Methods and Applications for COVID-19 Disease Diagnosis and Risk Stratification serves as a vital resource for researchers in the areas of virology, metabolic diseases, respiratory disorders, as well as to clinical scientists, physicians, pharmacologists, and the healthcare services.
Preface v
Contributors xi
Part I Reviews
1 Multiplex Technologies in COVID-19 Research, Diagnostics, and Prognostics: Battling the Pandemic
3(18)
Paul C. Guest
Fatemeh Zahedipour
Muhammed Majeed
Tannaz Jamialahmadi
Amirhossein Sahebkar
2 Multivalent Vaccine Strategies in Battling the Emergence of COVID-19 Variants
21(16)
Paul C. Guest
3 Challenges of Multiplex Assays for COVID-19 Research: A Machine Learning Perspective
37(16)
Paul C. Guest
David Popovic
Johann Steiner
Part II Protocols
4 Multiplex Quantitative Polymerase Chain Reaction Diagnostic Test for SARS-CoV-2 and Influenza A/B Viruses
53(14)
Steve F. C. Hawkins
Paul C. Guest
5 Multiplex Quantitative Polymerase Chain Reaction Test to Identify SARS-CoV-2 Variants
67(12)
Steve F. C. Hawkins
Paul C. Guest
6 NIRVANA for Simultaneous Detection and Mutation Surveillance of SARS-CoV-2 and Co-infections of Multiple Respiratory Viruses
79(10)
Chongwei Bi
Gerardo Ramos-Mandujano
Mo Li
7 Quantitative Real-Time RT-PCR Systems to Detect SARS-CoV-2
89(10)
Sumino Yanase
Hiroyoshi Sasahara
Momoko Nabetani
Kensuke Yamazawa
Keisuke Aoyagi
Akiko Mita
Yuichi Honma
Yasuhiko Chiba
8 Guidance for SARS-CoV-2 RNA-Based Molecular Assay Analytical Performance Evaluations
99(18)
Lara Noble
Lesley Scott
Riffat Munir
Kim Steegen
Mignon du Plessis
Lucia Hans
Wendy Stevens
9 A Rapid User-Friendly Lab-on-a-Chip Microarray Platform for Detection of SARS-CoV-2 Variants
117(16)
Emily Mattig
Paul C. Guest
Harald Peter
10 SARS-CoV-2 Host Immunogenetic Biomarkers
133(16)
Maemu P. Gededzha
Nakampe Mampeule
Anastasia Gandini
Elizabeth S. Mayne
11 FnCas9 Editor Linked Uniform Detection Assay for COVID-19
149(12)
Rhythm Phutela
Sneha Gulati
Manoj Kumar
Souvik Maiti
Debojyoti Chakraborty
12 Mass Spectrometry Multiplexed Detection of SARS-CoV-2
161(14)
Luciana Godoy Viana
Adriana Lebkuchen
Rodrigo Andrade Schuch
Guilherme Goncalves Okai
Jessica Silva Salgueiro
Karina Helena Morals Cardozo
Valdemir Melechco Carvalho
13 Identification of Circulating Biomarkers of COVID-19 Using MALDI-TOF Mass Spectrometry
175(8)
Lucas C. Lazari
Livia Rosa-Fernandes
Giuseppe Palmisano
14 Antibody-Based Affinity Capture Combined with LC-MS Analysis for Identification of COVID-19 Disease Serum Biomarkers
183(18)
Paul C. Guest
Hassan Rahmoune
15 Liquid Chromatography-Mass Spectrometry Analysis of Peripheral Blood Mononuclear Cells from SARS-CoV-2 Infected Patients
201(12)
Paul C. Guest
Hassan Rahmoune
16 Assay of Fatty Acids and Their Role in the Prevention and Treatment of COVID-19
213(22)
Tharusha Jayasena
Sonia Bustamante
Anne Poljak
Perminder Sachdev
17 Lab-on-a-Chip Immunoassay for Prediction of Severe COVID-19 Disease
235(10)
Harald Peter
Emily Mattig
Paul C. Guest
Frank F. Bier
18 Multiplex Immunoassay for Prediction of Disease Severity Associated with the Cytokine Storm in COVID-19 Cases
245(12)
Paul C. Guest
Mitra Abbasifard
Tannaz Jamialahmadi
Muhammed Majeed
Prashant Kesharwani
Amirhossein Sahebkar
19 Detection of IgG Antibodies to SARS-CoV-2 and Neutralizing Capabilities Using the Luminex® xMAP® SARS-CoV-2 Multi-Antigen IgG Assay
257(16)
Abbe King
Gregory King
Christy Weiss
Sherry Dunbar
Shubhagata Das
20 Multiplex Testing of the Effect of Statins on Disease Severity Risk in COVID-19 Cases
273(12)
Fatemeh Zahedipour
Paul C. Guest
Muhammed Majeed
Khalid Al-Rasadi
Tannaz Jamialahmadi
Amirhossein Sahebkar
21 Evaluating the Effects of Curcumin on the Cytokine Storm in COVID-19 Using a Chip-Based Multiplex Analysis
285(12)
Fatemeh Zahedipour
Paul C. Guest
Muhammed Majeed
Seyed Adel Moallem
Prashant Kesharwani
Tannaz Jamialahmadi
Amirhossein Sahebkar
22 COVID-19 Detection Using the NHS Lateral Flow Test Kit
297(10)
Paul C. Guest
Hassan Rahmoune
23 Evaluation Protocol for SARS-CoV-2 Serological Assays
307(14)
Maemu P. Gededzha
Sarika Jugwanth
Nakampe Mampeule
Nontobeko Zwane
Anura David
Lesley Scott
Wendy Stevens
Elizabeth S. Mayne
24 Measurement of Mitochondrial Respiration in Cryopreserved Human Peripheral Blood Mononuclear Cells (PBMCs)
321(12)
Keiko Iwata
Min-Jue Xie
Paul C. Guest
Takaharu Hirai
Hideo Matsuzazki
25 Multiplex Testing of Oxidative-Reductive Pathway in Patients with COVID-19
333(12)
Paul C. Guest
Mitra Abbasifard
Tannaz Jamialahmadi
Muhammed Majeed
Prashant Kesharwani
Amirhossein Sahebkar
26 Point-of-Care Device for Assessment of Blood Coagulation Status in COVID-19 Patients
345(10)
Paul C. Guest
Hassan Rahmoune
27 COVID-19 and the Assessment of Coenzyme Q10
355(12)
Nadia Turton
Robert A. Heaton
Iain P. Hargreaves
28 Isolation and Cell Culture of Human Nasopharyngeal Cells: A Model for Testing Immune Response and Antiviral Treatment
367(8)
Krist Helen Antunes
Ana Paula Duarte de Souza
29 Machine Learning Approaches to Analyze MALDI-TOF Mass Spectrometry Protein Profiles
375(20)
Lucas C. Lazari
Livia Rosa-Fernandes
Giuseppe Palmisano
30 A Deep Learning Approach to Identify Chest Computed Tomography Features for Prediction of SARS-CoV-2 Infection Outcomes
395(12)
Amirhossein Sahebkar
Mitra Abbasifard
Samira Chaibakhsh
Paul C. Guest
Mohamad Amin Pourhoseingholi
Amir Vahedian-Azimi
Prashant Kesharwani
Tannaz Jamialahmadi
Part III Future Perspectives
31 Genomic Surveillance for Monitoring Variants of Concern: SARS-CoV-2 Delta, Omicron, and Beyond
407(8)
Paul C. Guest
Index 415