E-raamat: Natural Product Isolation and Identification: Methods and Protocols

Natural products analysis through time: from past achievements to future prospect.- Optimizing extraction solvents for plant chemical analysis using 1H NMR and principal component analysis.- High-resolution isolation of natural products: efficient combination of dryload injection and HPLC gradient transfer.- Standardized HPTLC for reproducible Chemical Fingerprinting.- Extraction of anthocyanins with deep eutectic solvents and chemical evaluation by high performance liquid chromatography.- Extraction of essential oils and terpene volatiles from plants and identification by GC-MS based techniques.- Isolation of natural products from marine invertebrates.- Identification and profiling of primary metabolites through GC-MS and associated data processing.- Identification of flavonoids using UV-VIS and MS spectra.- Analysis of salicylate and phenolic acids in plant by HPLC-fluorescence detector.- Isolation and identification of coumarins from Citrus latifolia leaves using advanced chromatographic techniques.- Identification of Lignans.- Extraction, annotation, and purification of resin glycosides from the morning glory family (Convolvulaceae).- Extraction, isolation and structure elucidation of acylated triterpene saponins from Asteraceae family.- Analysis and identification of polyphenols in Fragaria, Rubus and Ribes species by non-targeted UHPLC-PDA-MS metabolite profiling.- Identification of primary metabolites in 1H NMR-based metabolomics of plants.- Isolation of natural products using preparative-TLC.- Sample preparation of Caenorhabditis elegans for GC-MS based metabolomics in toxicity assessment.