E-raamat: Notch Signaling Research: Methods and Protocols

Using the CRISPR/Cas9 System for Dissection of Functional Sites of the Notch Gene in Drosophila melanogaster.- Screening Mutants by Single Fly Genomic PCR.- Generation of Properly Folded Epidermal Growth Factor-Like (EGF) Repeats and Glycosyltransferases Enables In Vitro O-Glycosylation.- Use of FLP/FRT System to Screen for Notch Signaling Regulators in the Drosophila Wing.- High-Throughput Analysis to Identify Activators of Notch Signaling.- Artificial Notch Signaling Activation Method Using Immobilized Ligand Beads.- Mammalian NOTCH Receptors Activation and Signaling Protocols.- Somatic Clonal Analyses Using FLP/FRT and MARCM System to Understand Notch Signaling Mechanism and Its Regulation.- Analyzing the Interaction of RBPJ with Mitotic Chromatin and Its Impact on Transcription Reactivation upon Mitotic Exit.- Assessing the Roles of Potential Notch Signaling Components in Instructive and Permissive Pathways with Two Drosophila Pericardial Reporters.- Image-Based Single-Molecule Analysis of Notch-Dependent Transcription in Its Natural Context.- An Automatic Stage Identification MATLAB Tool to Reveal Notch Expression Pattern in Drosophila Egg Chambers.- Employing the CRISPR Technology for Studying Notch Signaling in the Male Gonad of Drosophila melanogaster.- Immuno-Localization of Notch Signaling in Mouse Preimplantation Embryos.- Studying the NOTCH Signaling Pathway Activation in Kidney Biopsies.- Exosomes as Carriers for Notch Molecules.- In Vivo and Ex Vivo Experimental Approach for Studying Functional Role of Notch in Pulmonary Vascular Disease.- Metastasis Model to Test the Role of Notch Signaling in Prostate Cancer.- Functional Studies of Genetic Variants Associated with Human Diseases in Notch Signaling-Related Genes Using Drosophila.- Bioinformatics Tools to Understand Notch.