| Preface |
|
xiii | |
| Acknowledgments |
|
xv | |
|
|
|
1 | (24) |
|
1.1 Light: A Brief Introduction to its Properties |
|
|
2 | (3) |
|
|
|
2 | (3) |
|
1.2 An Explanation of Energy, Wavelength, and Frequency Jargon |
|
|
5 | (1) |
|
|
|
6 | (4) |
|
|
|
10 | (2) |
|
1.5 Near-Field and Far-Field |
|
|
12 | (1) |
|
1.6 Light--Matter Interactions |
|
|
12 | (1) |
|
|
|
13 | (2) |
|
1.8 Total Internal Reflection |
|
|
15 | (2) |
|
|
|
17 | (1) |
|
|
|
18 | (1) |
|
|
|
18 | (1) |
|
|
|
19 | (3) |
|
|
|
22 | (1) |
|
|
|
22 | (1) |
|
1.15 Real Versus Reciprocal Space |
|
|
23 | (1) |
|
1.16 Further Reading on Optics |
|
|
24 | (1) |
|
2 Introduction to Common Optical Components |
|
|
25 | (85) |
|
|
|
25 | (7) |
|
|
|
32 | (16) |
|
2.2.1 White Light Sources |
|
|
33 | (1) |
|
|
|
34 | (1) |
|
|
|
34 | (5) |
|
2.2.4 Light Emitting Diodes |
|
|
39 | (1) |
|
|
|
39 | (3) |
|
|
|
42 | (1) |
|
2.2.7 Optical Parametric Oscillator |
|
|
43 | (1) |
|
2.2.8 Optical Parametric Amplifier |
|
|
43 | (1) |
|
2.2.9 Characterizing Your Light |
|
|
44 | (2) |
|
|
|
46 | (2) |
|
2.3 Common Components in an Optics Lab |
|
|
48 | (58) |
|
|
|
48 | (5) |
|
|
|
53 | (1) |
|
|
|
54 | (1) |
|
|
|
55 | (3) |
|
|
|
58 | (1) |
|
|
|
59 | (1) |
|
|
|
59 | (2) |
|
2.3.8 L-Brackets or Table Clamps |
|
|
61 | (1) |
|
2.3.9 Removable/Flip/Kinematic Holders |
|
|
61 | (1) |
|
|
|
62 | (1) |
|
|
|
63 | (2) |
|
|
|
65 | (1) |
|
|
|
65 | (4) |
|
|
|
69 | (2) |
|
|
|
71 | (2) |
|
2.3.16 Polarization Scramblers |
|
|
73 | (1) |
|
|
|
73 | (1) |
|
2.3.18 Filters and Dichroic Mirrors |
|
|
74 | (5) |
|
2.3.19 Apertures, Irises, and Pinholes |
|
|
79 | (1) |
|
2.3.20 Beam Blocks, Traps, and Shutters |
|
|
80 | (1) |
|
|
|
80 | (2) |
|
2.3.22 Antireflection Coatings |
|
|
82 | (1) |
|
|
|
82 | (3) |
|
|
|
85 | (1) |
|
2.3.25 Microscope Objectives |
|
|
85 | (1) |
|
|
|
86 | (1) |
|
|
|
86 | (1) |
|
|
|
87 | (1) |
|
2.3.29 Infinity Corrected Objectives |
|
|
87 | (1) |
|
2.3.30 Numerical Aperture |
|
|
87 | (1) |
|
2.3.31 Immersion Objectives |
|
|
88 | (1) |
|
|
|
89 | (1) |
|
|
|
89 | (1) |
|
2.3.34 Long Working Distance Objectives |
|
|
90 | (1) |
|
|
|
91 | (1) |
|
2.3.36 Diffraction Gratings |
|
|
91 | (1) |
|
|
|
92 | (2) |
|
2.3.38 Spectrometers (Monochromators) |
|
|
94 | (3) |
|
|
|
97 | (1) |
|
|
|
97 | (1) |
|
|
|
98 | (1) |
|
2.3.42 Photomultiplier Tube |
|
|
98 | (1) |
|
|
|
99 | (1) |
|
2.3.44 Mercury Cadmium Telluride Detectors |
|
|
99 | (1) |
|
|
|
100 | (1) |
|
|
|
100 | (1) |
|
2.3.47 Charge Coupled Device |
|
|
100 | (1) |
|
2.3.48 Electron Multiplying Charge Coupled Device |
|
|
101 | (1) |
|
2.3.49 Scientific Complementary Metal Oxide Semiconductor |
|
|
101 | (1) |
|
|
|
102 | (1) |
|
2.3.51 Alignment Tools: Cards, Viewers, and Targets |
|
|
102 | (1) |
|
2.3.52 Acousto-Optical Tunable Filter |
|
|
103 | (1) |
|
2.3.53 Acousto-Optical Modulators |
|
|
103 | (1) |
|
2.3.54 Electro Optic Modulators (EOMs) |
|
|
103 | (1) |
|
|
|
104 | (1) |
|
2.3.56 Nonlinear Crystals |
|
|
104 | (1) |
|
|
|
105 | (1) |
|
|
|
106 | (2) |
|
2.4.1 Microlenses and Spectrometers |
|
|
106 | (1) |
|
|
|
107 | (1) |
|
|
|
107 | (1) |
|
2.5 Further Reading on Optical Components |
|
|
108 | (1) |
|
|
|
108 | (2) |
|
3 Spectroscopy: So Many Squiggly Lines! |
|
|
110 | (79) |
|
3.1 UV-VIS-NIR Absorption Spectroscopy |
|
|
113 | (5) |
|
3.2 Fluorescence Spectroscopy |
|
|
118 | (5) |
|
3.3 Photoluminescence Spectroscopy (and an Early Introduction to Imaging) |
|
|
123 | (14) |
|
3.4 Fourier Transform Infrared Spectroscopy |
|
|
137 | (20) |
|
|
|
142 | (3) |
|
3.4.2 Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy |
|
|
145 | (1) |
|
3.4.3 Specular Reflection |
|
|
146 | (1) |
|
|
|
146 | (2) |
|
3.4.5 Diffuse Reflectance Fourier Transform Infrared Spectroscopy |
|
|
148 | (2) |
|
3.4.6 Baseline Correction |
|
|
150 | (1) |
|
3.4.7 Atmospheric Compensation |
|
|
151 | (1) |
|
|
|
151 | (1) |
|
|
|
151 | (2) |
|
|
|
153 | (1) |
|
3.4.11 Difference Spectra |
|
|
153 | (1) |
|
3.4.12 Chemometrics and Multivariate Image Analysis |
|
|
153 | (1) |
|
|
|
154 | (1) |
|
3.4.14 Quantum Cascade Lasers as FTIR Light Sources |
|
|
154 | (1) |
|
3.4.15 Coupling Scanning Probe Techniques with FTIR Spectroscopy |
|
|
155 | (1) |
|
3.4.16 Infrared Tomography |
|
|
155 | (1) |
|
3.4.17 Sum Frequency Generation Vibrational Spectroscopy |
|
|
156 | (1) |
|
|
|
157 | (17) |
|
3.5.1 Excitation Laser Selection |
|
|
158 | (1) |
|
3.5.2 Diffraction Grating Selection |
|
|
159 | (1) |
|
|
|
160 | (1) |
|
3.5.4 Number of Measurements Averaged Together |
|
|
161 | (1) |
|
|
|
161 | (4) |
|
|
|
165 | (1) |
|
3.5.7 Baseline Correction |
|
|
165 | (1) |
|
|
|
165 | (1) |
|
3.5.9 Polarized Raman Spectroscopy |
|
|
166 | (2) |
|
|
|
168 | (1) |
|
3.5.11 Confocal Raman 3D Imaging |
|
|
169 | (1) |
|
|
|
169 | (1) |
|
3.5.13 Coherent Anti-Stokes Raman Spectroscopy |
|
|
170 | (2) |
|
3.5.14 Stimulated Raman Spectroscopy |
|
|
172 | (1) |
|
3.5.15 Surface Enhanced Raman Spectroscopy |
|
|
173 | (1) |
|
3.5.16 Tip Enhanced Raman Spectroscopy |
|
|
173 | (1) |
|
3.6 Laser Induced Breakdown Spectroscopy |
|
|
174 | (2) |
|
3.7 Hyperspectral Imaging/Mapping |
|
|
176 | (3) |
|
3.8 Ultrafast, Pump-Probe Time-Resolved Measurements |
|
|
179 | (4) |
|
3.9 Chemometrics: Multivariate Data Analysis |
|
|
183 | (4) |
|
3.9.1 Principal Component Analysis |
|
|
184 | (1) |
|
|
|
185 | (1) |
|
3.9.3 Classical Least Squares |
|
|
186 | (1) |
|
3.9.4 Partial Least Squares |
|
|
186 | (1) |
|
|
|
187 | (1) |
|
|
|
187 | (1) |
|
3.10 Further Reading About Spectroscopy Techniques |
|
|
187 | (2) |
|
4 Optical Imaging: What Are the Pretty Pictures Actually Showing Me? |
|
|
189 | (78) |
|
4.1 A Quick Tour of an Optical Microscope |
|
|
189 | (14) |
|
4.1.1 Kohler illumination |
|
|
198 | (1) |
|
4.1.2 Controlling a Microscope System |
|
|
199 | (1) |
|
|
|
200 | (2) |
|
|
|
202 | (1) |
|
|
|
203 | (2) |
|
|
|
205 | (3) |
|
4.4 Phase Contrast Imaging |
|
|
208 | (5) |
|
4.5 Cross Polarized Imaging |
|
|
213 | (2) |
|
4.6 Differential Interference Contrast Imaging |
|
|
215 | (5) |
|
4.7 Wide Field Fluorescence Imaging |
|
|
220 | (5) |
|
4.7.1 Sample Preparation for Fluorescent Imaging |
|
|
220 | (5) |
|
4.8 Total Internal Reflection Fluorescence Microscopy |
|
|
225 | (2) |
|
|
|
227 | (3) |
|
4.10 Light Sheet Microscopy or Selective Plane Illumination Microscopy |
|
|
230 | (3) |
|
4.11 Multiphoton Microscopy |
|
|
233 | (4) |
|
4.11.1 Second Harmonic Generation |
|
|
235 | (1) |
|
4.11.2 Two-Photon Fluorescence |
|
|
235 | (2) |
|
4.12 Fluorescence Lifetime Imaging Microscopy/Time Resolved Photoluminescence Imaging/Time Correlated Single-Photon Counting |
|
|
237 | (3) |
|
|
|
238 | (1) |
|
4.12.2 Frequency Domain FLIM |
|
|
239 | (1) |
|
4.12.3 Phasor Analysis of FLIM Data |
|
|
240 | (1) |
|
4.13 Introduction to Super Resolution Microscopy |
|
|
240 | (2) |
|
|
|
242 | (1) |
|
4.15 Stimulated Emission Depletion Microscopy |
|
|
243 | (1) |
|
4.16 Structured Illumination Microscopy |
|
|
243 | (7) |
|
4.17 Stochastic Optical Reconstruction Microscopy and Photoactivated Localization Microscopy |
|
|
250 | (2) |
|
4.18 Atomic Force Microscopy: A Brief Overview |
|
|
252 | (3) |
|
4.19 Electron Microscopy: A Brief Overview |
|
|
255 | (9) |
|
4.20 Photolithography: A Brief Overview |
|
|
264 | (2) |
|
4.21 Further Reading on Microscopy Techniques |
|
|
266 | (1) |
|
5 Notes on How to Design and Build Optical Setups in the Lab |
|
|
267 | (17) |
|
|
|
270 | (1) |
|
|
|
271 | (2) |
|
5.3 Inserting a Lens or Other Optical Element |
|
|
273 | (2) |
|
|
|
275 | (1) |
|
|
|
276 | (1) |
|
|
|
277 | (1) |
|
5.7 Prism Pulse Compressors |
|
|
278 | (2) |
|
5.8 Using a Microscope Slide as a Beam Splitter |
|
|
280 | (1) |
|
|
|
281 | (3) |
|
|
|
284 | (9) |
|
Appendix 1 The Photoelectric Effect and Photoelectron Spectroscopy |
|
|
284 | (3) |
|
Appendix 2 Wave-Particle Duality |
|
|
287 | (2) |
|
Appendix 3 Young's Double Slit Experiment |
|
|
289 | (2) |
|
Appendix 4 Blackbody Radiation |
|
|
291 | (2) |
| Index |
|
293 | |