E-raamat: PCR Protocols

Part I. Introduction to PCR A Short History of the Polymerase Chain
Reaction John M. S. Bartlett and David Stirling PCR Patent Issues Peter
Carroll and David Casimir Equipping and Establishing a PCR Laboratory Susan
McDonagh Quality Control in PCR David Stirling Part II. Preparation of
Nucleic Acid Templates Extraction of Nucleic Acid Templates John M. S.
Bartlett Extraction of DNA from Whole Blood John M. S. Bartlett and Anne
White DNA Extraction from Tissue Helen Pearson and David Stirling
Extraction of DNA from Microdissected Archival Tissues James J. Going RNA
Extraction from Blood Helen Pearson RNA Extraction from Frozen Tissue John
M. S. Bartlett RNA Extraction from Tissue Sections Helen Pearson Dual
DNA/RNA Extraction David Stirling and John M. S. Bartlett DNA Extraction
from Fungi, Yeast, and Bacteria David Stirling Isolation of RNA Viruses from
Biological Materials Susan McDonagh Extraction of Ancient DNA Wera M.
Schmerer DNA Extraction from Plasma and Serum David Stirling Technical
Notes for the Detection of Nucleic Acids John M. S. Bartlett Technical Notes
for the Recovery and Purification of PCR Products from Acrylamide Gels David
Stirling Part III. Basic PCR Methods PCR Primer Design David L. Hyndman and
Masato Mitsuhashi Optimization of Polymerase Chain Reactions Haiying
Grunenwald Subcycling PCR for Long-Distance Amplifications of Regions with
High and Low Guanine-Cystine Content: Amplification of the Intron 22
Inversion of the FVIII Gene David Stirling Rapid Amplification of cDNA Ends
Xin Wang and W. Scott Young III Randomly Amplified Polymorphic DNA
Fingerprinting: The Basics Ranil S. Dassanayake and Lakshman P. Samaranayake
Microsphere-Based Single Nucleotide Polymorphism Genotyping Marie A. Iannone,
J. David Taylor, Jingwen Chen, May-Sung Li, Fei Ye, and Michael P. Weiner
Ligase Chain Reaction William H. Benjamin, Jr., Kim R. Smith, and Ken B.
Waites Nested RT-PCR in a Single Closed Tube Antonio Olmos, Olga Esteban,
Edson Bertolini, and Mariano Cambra Direct PCR from Serum: Application to
Viral Genome Detection Kenji Abe Long PCR Amplification of Large Fragments
of Viral Genomes: A Technical Overview Raymond Tellier, Jens Bukh, Suzanne U.
Emerson, and Robert H. Purcell Long PCR Methodology Raymond Tellier, Jens
Bukh, Suzanne U. Emerson, and Robert H. Purcell Part IV. Ultrasensitive and
Quantitative PCR Qualitative and Quantitative PCR: A Technical Overview
David Stirling Ultrasensitive PCR Detection of Tumor Cells in Myeloma
Friedrich W. Cremer and Marion Moos Ultrasensitive Quantitative PCR to
Detect RNA Viruses Susan McDonagh Quantitative PCR for cAMP RI Alpha mRNA:
Use of Site-Directed Mutation and PCR Mimics John M. S. Bartlett
Quantitation of Multiple RNA Species Ron Kerr Part V. Transcriptome Analysis
Differential Display: A Technical Overview John M. S. Bartlett
AU-Differential Display, Reproducibility of a Differential mRNA Display
Targeted to AU Motifs Orlando Dominguez, Lidia Sabater, Yaqoub Ashhab, Eva
Belloso, and Ricardo Pujol-Borrell PCR Fluorescence Differential Display
Kostya Khalturin, Sergej Kuznetsov, and Thomas C. G. Bosch Microarray
Analysis Using RNA Arbitrarily Primed PCR Steven Ringquist, Gaelle Rondeau,
Rosa-Ana Risques, Takuya Higashiyama, Yi-Peng Wang, Steffen Porwollik, David
Boyle, Michael McClelland, and John Welsh Oligonucleotide Arrays for
Genotyping: Enzymatic Methods for Typing Single Nucleotide Polymorphisms and
Short Tandem Repeats Stephen Case-Green, Clare Pritchard, and Edwin Southern
Serial Analysis of Gene Expression Karin A. Oien Part VI. Mutations and
Polymorphisms Mutation and Polymorphism Detection: A Technical Overview
Joanne Edwards and John M. S. Bartlett Combining Multiplex and Touchdown PCR
for Microsatellite Analysis Kanokporn Rithidech and John J. Dunn Detection
of Microsatellite Instability and Loss of Heterozygosity Using DNA Extracted
from Formalin-Fixed Paraffin-Embedded Tumor Material by Fluorescence-Based
Multiplex Microsatellite PCR Joanne Edwards and John M. S. Bartlett Reaction
of Shadow Band Synthesis During PCR Amplification of Repetitive Sequences
from Modern and Ancient DNA Wera M. Schmerer Degenerate
Oligonucleotide-Primed PCR Michaela Aubele and Jan Smida Mutation Detection
Using RT-PCR-RFLP Hitoshi Nakashima, Mitsuteru Akahoshi, and Yosuke Tanaka
Multiplex Amplification Refractory Mutation System for the Detection of
Prothrombotic Polymorphisms David Stirling PCR-SSCP Analysis of
Polymorphism: A Simple and Sensitive Method for Detecting Differences Between
Short Segments of DNA Mei Han and Mary Ann Robinson Part VII. PCR-Based
Sequencing Sequencing: A Technical Overview David Stirling Preparation and
Direct Automated Cycle Sequencing of PCR Products Susan E. Daniels
Nonradioactive PCR Sequencing Using Digoxigenin Siegfried Kosel, Christoph B.
Lucking, Rupert Egensperger, and Manuel B. Graeber Direct Sequencing by
Thermal Asymmetric PCR Georges-Raoul Mazars and Charles Theillet Analysis of
Nucleotide Sequence Variations by Solid-Phase Minisequencing Anu Suomalainen
and Ann-Christine Syvanen Direct Sequencing with Highly Degenerate and
Inosine-Containing Primers Zhiyuan Shen, Jingmei Liu, Robert L. Wells, and
Mortimer M. Elkind Determination of Unknown Genomic Sequences Without
Cloning Jean-Pierre Quivy and Peter B. Becker Cloning PCR Products for
Sequencing in M13 Vectors David Walsh DNA Rescue by the Vectorette Method
Marcia A. McAleer, Alison J. Coffey, and Ian Dunham Technical Notes for
Sequencing Difficult Templates David Stirling Part VIII. In Situ PCR and
PRINS PCR-Based Detection of Nucleic Acids in Chromosomes, Cells, and
Tissues: Technical Considerations on PRINS and In Situ PCR, and Comparison
with In Situ Hybridization Ernst J. M. Speel, Frans C. S. Ramaekers, and
Anton H. N. Hopman Cycling Primed In Situ Amplification John H. Bull and
Lynn Paskins Direct and Indirect In Situ PCR Klaus Hermann Wiedorn and
Torsten Goldmann Reverse Transcriptase In Situ PCR: New Methods in Cellular
Interrogation Mark Gilchrist and A. Dean Befus Primed In Situ Nucleic Acid
Labeling Combined with Immunocytochemistry to Simultaneously Localize DNA and
Proteins in Cells and Chromosomes Ernst J. M. Speel, Frans C. S. Ramaekers,
and Anton H. N. Hopman Part IX. Cloning and Mutagenesis Cloning and
Mutagenesis: A Technical Overview Helen Pearson and David Stirling Using T4
DNA Polymerase to Generate Clonable PCR Products Kai Wang A T-Linker
Strategy for Modification and Directional Cloning of PCR Products Robert M.
Horton, Raghavanpillai Raju, and Bianca M. Conti-Fine Cloning Gene Family
Members Using PCR with Degenerate Oligonucleotide Primers Gregory M. Preston
cDNA Libraries from a Low Amount of Cells Philippe Ravassard, Christine
Icard-Liepkalns, Jacques Mallet, and Jean Baptiste Dumas Milne Edwards
Creation of Chimeric Junctions, Deletions, and Insertions by PCR Genevieve
Pont-Kingdon Recombination and Site-Directed Mutagenesis Using Recombination
PCR Douglas H. Jones and Stanley C. Winistorfer Megaprimer PCR: Application
in Mutagenesis and Gene Fusion Emily Burke and Sailen Barik Index