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E-raamat: Peptide Microarrays: Methods and Protocols

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This expert volume provides insights into the technological fundamentals together with a comprehensive overview of the potentialities of peptide microarray technology in basic research and clinical assays. Advancements made in recent years in peptide library synthesis, immobilization chemistry and array production have created a foundation from which different new applications are derived, extending the ways in which peptide microarray technology is applied every day. Divided into three sections, the book covers cutting-edge methods and technology, chemoselective strategies for peptide immobilization, and peptide microarrays for medical applications. Written for the high successfulMethods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and tips on troubleshooting and avoiding known pitfalls.

Fully updated and authoritative, Peptide Microarrays: Methods and Protocols, Second Edition seeks to encourage scientists to apply current peptide array protocols to the study of interesting new biochemical and medical questions and to assist researchers aiming at developing new methods to further develop peptide microarray technology.

Preface v
Contributors ix
Part I Peptide Arrays: Cutting-Edge Methods and Technology
1 Peptide Arrays on Planar Supports
3(16)
Victor Tapia Mancilla
Rudolf Volkmer
2 High-Throughput Microarray Incubations Using Multi-Well Chambers
19(8)
Johannes Zerweck
Ulf Reimer
Janina Jansong
Nikolaus Pawlowski
Christoph Tersch
Maren Eckey
Tobias Knaute
3 Analysis of Protein Tyrosine Kinase Specificity Using Positional Scanning Peptide Microarrays
27(8)
Yang Deng
Benjamin E. Turk
4 Secondary Structure Determination of Peptides and Proteins After Immobilization
35(16)
Sulla H. North
Chris R. Taitt
5 Peptides and Anti-peptide Antibodies for Small and Medium Scale Peptide and Anti-peptide Affinity Microarrays: Antigenic Peptide Selection, Immobilization, and Processing
51(16)
Fan Zhang
Andrea Briones
Mikhail Soloviev
6 Low-Cost Peptide Microarrays for Mapping Continuous Antibody Epitopes
67(18)
Ryan McBride
Steven R. Head
Phillip Ordoukhanian
Mansun Law
7 The Peptide Microarray-Based Resonance Light Scattering Assay for Sensitively Detecting Intracellular Kinase Activity
85(12)
Tao Li
Xia Liu
Dianjun Liu
Zhenxin Wang
8 Anomalous Reflection of Gold: A Novel Platform for Biochips
97(14)
Amir Syahir
Kin-ya Tomizaki
Kotaro Kajikawa
Hisakazu Mihara
9 High-Throughput Peptide Screening on a Bimodal Imprinting Chip Through MS-SPRi Integration
111(16)
Weizhi Wang
Qiaojun Fang
Zhiyuan Hu
10 Analyzing Peptide Microarray Data with the R pepStat Pickage
127(18)
Gregory Imholte
Renan Sauteraud
Raphael Gottardo
Part II Chemoselective Strategies to Peptide Immobilization
11 Chemoselective Strategies to Peptide and Protein Bioprobes Immobilization on Microarray Surfaces
145(12)
Alessandro Gori
Renato Longhi
12 Manufacturing of Peptide Microarrays Based on Catalyst-Free Click Chemistry
157(10)
Denis Prim
Marc E. Pfeifer
13 Clickable Polymeric Coating for Oriented Peptide Immobilization
167(16)
Laura Sola
Alessandro Gori
Marina Cretich
Chiara Finetti
Caterina Zilio
Marcella Chiari
14 Oriented Peptide Immobilization on Microspheres
183(16)
Lisa C. Shriver-Lake
George P. Anderson
Chris R. Taitt
15 A Cell Microarray Format: A Peptide Release System Using a Photo-Cleavable Linker for Cell Toxicity and Cell Uptake Analysis
199(14)
Kenji Usui
Kin-ya Tomizaki
Hisakazu Mihara
Part III Peptide Microarrays for Medical Applications
16 Peptide Microarrays for Medical Applications in Autoimmunity, Infection, and Cancer
213(10)
Carsten Grotzinger
17 Synthetic Peptide-Based ELISA and ELISpot Assay for Identifying Autoantibody Epitopes
223(12)
Judit Pozsgay
Eszter Szarka
Krisztina Huber
Fruzsina Babos
Anna Magyar
Ferenc Hudecz
Gabriella Sarmay
18 IgE and IgG4 Epitope Mapping of Food Allergens with a Peptide Microarray Immunoassay
235(16)
Javier Martinez-Botas
Belen de la Hoz
19 IgE Epitope Mapping Using Peptide Microarray Immunoassay
251(12)
Gustavo Gimenez
Sara Benede
Jing Lin
20 Spot Synthesis: An Optimized Microarray to Detect IgE Epitopes
263(16)
Salvatore G. De-Simone
Paloma Napoleao-Pego
Thatiane S. De-Simone
21 Mapping of Epitopes Occurring in Bovine aαs1-Casein Variants by Peptide Microarray Immunoassay
279(18)
Maria Lisson
Georg Erhardt
Index 297