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E-raamat: Three Phase Partitioning: Applications in Separation and Purification of Biological Molecules and Natural Products

Edited by (Formerly post-doctoral positions: Massachusetts Institute of Technology, USA, University of Minnesota, USA, Lund University, Sweden, University of Technology of Compiegne, France), Edited by (Department of Biotechnology, National Institute of Pha)
  • Formaat: EPUB+DRM
  • Ilmumisaeg: 11-Aug-2021
  • Kirjastus: Elsevier Science Publishing Co Inc
  • Keel: eng
  • ISBN-13: 9780323859035
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  • Formaat: EPUB+DRM
  • Ilmumisaeg: 11-Aug-2021
  • Kirjastus: Elsevier Science Publishing Co Inc
  • Keel: eng
  • ISBN-13: 9780323859035
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Three Phase Partitioning: Applications in Separation and Purification of Biological Molecules and Natural Products presents applications in diverse areas of both chemical technology and biotechnology. This book serves as a single resource for learning about both the economical, facile and scalable processes, along with their potential for applications in the separation and purification of materials and compounds across the entire spectra of chemical and biological nature. The book begins by explaining the origins and fundamentals of TPP and continues with chapters on related applications, ranging from the purification of parasite recombinant proteases to oil extraction from oilseeds and oleaginous microbes, and more.

  • Written by researchers who have been pioneers in developing and utilizing three phase partitioning
  • Focuses on applications, with chapters detailing relevance to a wide variety of areas and numerous practical examples
  • Designed to give laboratory workers the information needed to undertake the challenge of designing successful three-phase partitioning protocols
Contributors ix
Preface xi
The journey in understanding interactions of salts and solvents with proteins continues! xiii
1 Three phase partitioning: some reminiscences, some science
1(8)
Clive Dennison
1.1 The origin of the TPP method
1(1)
1.2 How does TPP work?
2(1)
1.3 Inhibition of enzyme activity by t-BuOH
3(1)
1.4 Enhanced activity
4(1)
1.5 Other molecules
4(1)
1.6 TPP doesn't work in all cases
4(5)
References
5(4)
2 How and why we happen to use three phase partitioning in areas other than protein purification
9(14)
Munishwar Nath Gupta
2.1 TPP for purification of proteins/enzymes
11(4)
2.2 TPP used for edible oil extraction
15(2)
2.3 TPP of polysaccharides
17(1)
2.4 TPP of microbial cells
18(1)
2.5 Isolation and purification of low molecular weight compounds
19(1)
2.6 Conclusion
19(4)
References
20(3)
3 Fundamental aspects of protein isolation and purification
23(36)
John H.T. Luong
3.1 Introduction
23(2)
3.2 Fusion tags and protein solubility
25(1)
3.3 Cell lysis
26(5)
3.4 Non-mechanical procedures
31(3)
3.5 Protein precipitation
34(6)
3.6 Affinity precipitation and immunoprecipitation
40(1)
3.7 Protein purification
40(10)
3.8 Conclusions and outlooks
50(9)
References
53(6)
4 The multiple facets of three-phase partitioning in the purification, concentration, yield and activity of enzymes and proteins
59(20)
James Philip Dean Goldring
4.1 Introduction
59(2)
4.2 TPP as a rapid single step procedure to isolate and concentrate proteins
61(3)
4.3 TPP concentrates proteins by decreasing the volume of water
64(1)
4.4 TPP concentrates individual proteins by removing unwanted proteins
65(1)
4.5 TPP preserves and increases enzyme activity
66(1)
4.6 Explanations for TPP preserving and increasing enzyme activity
67(1)
4.7 Purification and/or refolding of denatured enzymes with TPP
68(2)
4.8 TPP purification of recombinant HIS-Tag fusion proteins or metal binding proteins
70(1)
4.9 TPP purification of proteins with affinity ligands
71(1)
4.10 Ultrasound assisted TPP to isolate proteins, oils and polysaccharides
71(1)
4.11 Microwave assisted TPP
72(1)
4.12 Versatility of TPP. separating DNA, carbohydrates and oils and two-step TPP protocols
72(1)
4.13 Conclusion
73(6)
References
74(5)
5 Enzymes recovery by three phase partitioning
79(32)
Mohammed Gagaoua
5.1 Introduction
79(3)
5.2 Overview of the studies and conditions of use of three phase partitioning and its va ria nts for the recovery of enzymes
82(12)
5.3 Three phase partitioning for the recovery of glycosidases
94(4)
5.4 Three phase partitioning for the recovery of proteases
98(3)
5.5 Three phase partitioning for the recovery of oxidoreductases
101(1)
5.6 Three phase partitioning for the recovery of lipases
102(1)
5.7 Three phase partitioning for the recovery of other hydrolases
103(1)
5.8 Conclusion
103(8)
References
104(7)
6 Emulsion gel formation in three phase partitioning
111(22)
R. Borbas
E. Kiss
6.1 Introduction
111(4)
6.2 The mechanism of TPP
115(13)
6.3 Conclusion
128(5)
References
130(3)
7 Three-phase partitioning (TPP) of proteases from parasites, plants, tissue and bacteria for enhanced activity
133(22)
Lauren E-A Eyssen
James Philip Dean Goldring
Theresa Helen Taillefer Coetzer
7.1 Why we are interested in proteases
133(7)
7.2 Three-phase partitioning as a protease purification tool
140(1)
7.3 Conditions considered during optimization of TPP
141(8)
7.4 Effect of TPP on protease structure and activity
149(1)
7.5 Conclusions
150(5)
References
151(4)
8 Three phase partitioning of plant peroxidases
155(20)
Yonca Duman
8.1 Peroxidases
155(1)
8.2 Sources and functions of peroxidases
156(3)
8.3 Biotechnological applications of plant peroxidases
159(2)
8.4 Three phase partitioning system
161(4)
8.5 TPP as an emerging technique for plant peroxidase purification
165(2)
8.6 Conclusion
167(8)
References
168(7)
9 Macro-(affinity ligand) facilitated three phase partitioning
175(22)
Ipsita Roy
Munishwar Nath Gupta
9.1 Introduction
175(2)
9.2 Water soluble polymers and smart polymers
177(6)
9.3 Smart biocatalysts
183(3)
9.4 MLFTPP
186(3)
9.5 Conclusion/future perspectives
189(8)
References
191(6)
10 Applications of three phase partitioning and macro-(aff inity ligand) facilitated three phase partitioning in protein refolding
197(26)
Munishwar Nath Gupta
Ipsita Roy
10.1 Introduction
197(19)
10.2 Conclusion
216(7)
References
217(6)
11 Three phase partitioning-based strategies for highly efficient separation of bioactive polysaccharides from natural resources
223(20)
Jing-Kun Yan
11.1 Introduction
223(6)
11.2 Factors affecting the TPP process for extraction of PSs
229(3)
11.3 Process intensification of TPP system for PSs extraction
232(4)
11.4 TPP combined with downstream techniques
236(1)
11.5 TPP separation influencing the properties of PSs
237(2)
11.6 Conclusions
239(4)
Acknowledgements
240(1)
References
240(3)
12 Technologies for oil extraction from oilseeds and oleaginous microbes
243(24)
S. P. Jeevan Kumar
Vljay Kumar Garlapati
Lohit Kumar Srinivas Gujjala
Rintu Banerjee
12.1 Introduction
243(2)
12.2 Importance of oil and lipid extraction
245(1)
12.3 Green solvents and techniques for oil and lipid extraction
246(2)
12.4 Green solvents for oil/lipid extraction
248(3)
12.5 Conventional and green extraction techniques for oil/lipid extraction
251(9)
12.6 Conclusion
260(7)
Author's contributions
261(1)
Competing interest
261(1)
Acknowledgements
261(1)
References
261(6)
13 Three phase partitioning (TPP) as an extraction technique for oleaginous materials
267(18)
Sandesh J. Marathe
Nirali N. Shah
Rekha S. Singhal
13.1 Introduction
267(1)
13.2 Conventional extraction techniques for oleaginous material
268(1)
13.3 Mechanism of extraction using TPP
269(2)
13.4 Advantages of TPP
271(1)
13.5 Factors affecting TPP
272(4)
13.6 Hyphenated TPP-techniques
276(5)
13.7 Challenges and future perspectives
281(4)
References
281(4)
14 Intensification of extraction of biomolecules using three-phase partitioning
285(28)
Sujata S. Patil
Virendra K. Rathod
14.1 Introduction
285(3)
14.2 Key factors affecting the TPP method
288(4)
14.3 Advanced TPP processes
292(3)
14.4 Process intensification of TPP
295(13)
14.5 Application of TPP for extraction and purification of biomolecules
308(4)
14.6 Challenges in TPP
312(1)
14.7 Conclusions
313(1)
References 313(10)
Index 323
Dr. Munishwar Nath Gupta earned his PhD from Indian Institute of Science, Bengaluru, and completed post-doctoral positions at Massachusetts Institute of Technology (USA), University of Minnesota (USA), Lund University (Sweden), and University of Technology of Compiegne (France). He has taught chemistry, biochemistry, and biotechnology at Indian Institute of Technology, Delhi, between 1975-2016. He was awarded the National Science Talent fellowship (India) and Fellowships of National Academy of Sciences and Indian National Science Academy. He has edited three books on thermostability of enzymes, non-aqueous enzymology and affinity-based separation methods (published by Springer/Birkhauser). He was an Associate Editor of Biocatalysis and Biotransformation (Taylor and Francis) and founding and former editor-in-chief of Sustainable Chemical Processes (Springer). Hes served on editorial boards of several national and international journals and acted as a consultant to Novozyme (Denmark), Dabur (India), and other international companies. His research interests include applied biocatalysis and interfaces of biochemistry with nanotechnology. Dr. Ipsita Roy earned her PhD from Indian Institute of Technology, Delhi, completed a post-doctoral position at Indian Institute of Technology, Delhi, and conducted an Alexander von Humboldt research fellowship at the University of Bonn (Germany). She has been teaching biotechnology and biochemistry at the postgraduate level at National Institute of Pharmaceutical Education and Research, S.A.S. Nagar, since 2005. Her research interests include stabilization of pharmaceutically important and disease-relevant proteins; aptamer technology; downstream processing of proteins.