Contributors |
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ix | |
Preface |
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xi | |
The journey in understanding interactions of salts and solvents with proteins continues! |
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xiii | |
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1 Three phase partitioning: some reminiscences, some science |
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1 | (8) |
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1.1 The origin of the TPP method |
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1 | (1) |
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2 | (1) |
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1.3 Inhibition of enzyme activity by t-BuOH |
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3 | (1) |
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4 | (1) |
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4 | (1) |
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1.6 TPP doesn't work in all cases |
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4 | (5) |
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5 | (4) |
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2 How and why we happen to use three phase partitioning in areas other than protein purification |
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9 | (14) |
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2.1 TPP for purification of proteins/enzymes |
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11 | (4) |
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2.2 TPP used for edible oil extraction |
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15 | (2) |
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2.3 TPP of polysaccharides |
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17 | (1) |
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2.4 TPP of microbial cells |
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18 | (1) |
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2.5 Isolation and purification of low molecular weight compounds |
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19 | (1) |
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19 | (4) |
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20 | (3) |
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3 Fundamental aspects of protein isolation and purification |
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23 | (36) |
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23 | (2) |
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3.2 Fusion tags and protein solubility |
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25 | (1) |
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26 | (5) |
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3.4 Non-mechanical procedures |
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31 | (3) |
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3.5 Protein precipitation |
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34 | (6) |
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3.6 Affinity precipitation and immunoprecipitation |
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40 | (1) |
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40 | (10) |
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3.8 Conclusions and outlooks |
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50 | (9) |
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53 | (6) |
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4 The multiple facets of three-phase partitioning in the purification, concentration, yield and activity of enzymes and proteins |
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59 | (20) |
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James Philip Dean Goldring |
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59 | (2) |
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4.2 TPP as a rapid single step procedure to isolate and concentrate proteins |
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61 | (3) |
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4.3 TPP concentrates proteins by decreasing the volume of water |
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64 | (1) |
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4.4 TPP concentrates individual proteins by removing unwanted proteins |
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65 | (1) |
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4.5 TPP preserves and increases enzyme activity |
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66 | (1) |
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4.6 Explanations for TPP preserving and increasing enzyme activity |
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67 | (1) |
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4.7 Purification and/or refolding of denatured enzymes with TPP |
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68 | (2) |
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4.8 TPP purification of recombinant HIS-Tag fusion proteins or metal binding proteins |
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70 | (1) |
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4.9 TPP purification of proteins with affinity ligands |
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71 | (1) |
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4.10 Ultrasound assisted TPP to isolate proteins, oils and polysaccharides |
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71 | (1) |
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4.11 Microwave assisted TPP |
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72 | (1) |
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4.12 Versatility of TPP. separating DNA, carbohydrates and oils and two-step TPP protocols |
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72 | (1) |
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73 | (6) |
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74 | (5) |
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5 Enzymes recovery by three phase partitioning |
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79 | (32) |
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79 | (3) |
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5.2 Overview of the studies and conditions of use of three phase partitioning and its va ria nts for the recovery of enzymes |
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82 | (12) |
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5.3 Three phase partitioning for the recovery of glycosidases |
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94 | (4) |
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5.4 Three phase partitioning for the recovery of proteases |
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98 | (3) |
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5.5 Three phase partitioning for the recovery of oxidoreductases |
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101 | (1) |
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5.6 Three phase partitioning for the recovery of lipases |
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102 | (1) |
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5.7 Three phase partitioning for the recovery of other hydrolases |
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103 | (1) |
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103 | (8) |
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104 | (7) |
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6 Emulsion gel formation in three phase partitioning |
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111 | (22) |
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111 | (4) |
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115 | (13) |
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128 | (5) |
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130 | (3) |
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7 Three-phase partitioning (TPP) of proteases from parasites, plants, tissue and bacteria for enhanced activity |
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133 | (22) |
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James Philip Dean Goldring |
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Theresa Helen Taillefer Coetzer |
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7.1 Why we are interested in proteases |
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133 | (7) |
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7.2 Three-phase partitioning as a protease purification tool |
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140 | (1) |
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7.3 Conditions considered during optimization of TPP |
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141 | (8) |
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7.4 Effect of TPP on protease structure and activity |
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149 | (1) |
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150 | (5) |
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151 | (4) |
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8 Three phase partitioning of plant peroxidases |
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155 | (20) |
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155 | (1) |
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8.2 Sources and functions of peroxidases |
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156 | (3) |
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8.3 Biotechnological applications of plant peroxidases |
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159 | (2) |
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8.4 Three phase partitioning system |
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161 | (4) |
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8.5 TPP as an emerging technique for plant peroxidase purification |
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165 | (2) |
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167 | (8) |
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168 | (7) |
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9 Macro-(affinity ligand) facilitated three phase partitioning |
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175 | (22) |
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175 | (2) |
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9.2 Water soluble polymers and smart polymers |
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177 | (6) |
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183 | (3) |
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186 | (3) |
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9.5 Conclusion/future perspectives |
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189 | (8) |
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191 | (6) |
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10 Applications of three phase partitioning and macro-(aff inity ligand) facilitated three phase partitioning in protein refolding |
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197 | (26) |
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197 | (19) |
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216 | (7) |
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217 | (6) |
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11 Three phase partitioning-based strategies for highly efficient separation of bioactive polysaccharides from natural resources |
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223 | (20) |
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223 | (6) |
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11.2 Factors affecting the TPP process for extraction of PSs |
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229 | (3) |
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11.3 Process intensification of TPP system for PSs extraction |
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232 | (4) |
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11.4 TPP combined with downstream techniques |
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236 | (1) |
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11.5 TPP separation influencing the properties of PSs |
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237 | (2) |
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239 | (4) |
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240 | (1) |
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240 | (3) |
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12 Technologies for oil extraction from oilseeds and oleaginous microbes |
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243 | (24) |
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Lohit Kumar Srinivas Gujjala |
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243 | (2) |
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12.2 Importance of oil and lipid extraction |
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245 | (1) |
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12.3 Green solvents and techniques for oil and lipid extraction |
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246 | (2) |
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12.4 Green solvents for oil/lipid extraction |
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248 | (3) |
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12.5 Conventional and green extraction techniques for oil/lipid extraction |
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251 | (9) |
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260 | (7) |
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261 | (1) |
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261 | (1) |
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261 | (1) |
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261 | (6) |
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13 Three phase partitioning (TPP) as an extraction technique for oleaginous materials |
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267 | (18) |
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267 | (1) |
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13.2 Conventional extraction techniques for oleaginous material |
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268 | (1) |
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13.3 Mechanism of extraction using TPP |
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269 | (2) |
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271 | (1) |
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13.5 Factors affecting TPP |
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272 | (4) |
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13.6 Hyphenated TPP-techniques |
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276 | (5) |
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13.7 Challenges and future perspectives |
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281 | (4) |
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281 | (4) |
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14 Intensification of extraction of biomolecules using three-phase partitioning |
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285 | (28) |
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285 | (3) |
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14.2 Key factors affecting the TPP method |
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288 | (4) |
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14.3 Advanced TPP processes |
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292 | (3) |
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14.4 Process intensification of TPP |
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295 | (13) |
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14.5 Application of TPP for extraction and purification of biomolecules |
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308 | (4) |
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312 | (1) |
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313 | (1) |
References |
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313 | (10) |
Index |
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323 | |