E-raamat: Phytophthora: Methods and Protocols

Promoter Analysis and Dissection Using Reporter Genes, Comparative Genomics,

and Gel Shift Assays in Phytophthora.- RNA Silencing Strategies in Phytophthora: Experimental Guidelines and Insights.- Protoplast Transformation of Phytophthora spp.- Gene Editing and Protein Tagging in the Oomycete Phytophthora infestans Using CRISPR-Cas12a.- CRISPR/Cas9-Mediated Gene-Knockout and In Situ Complementation System for Phytophthora sojae.- Fluorescence In Situ Hybridization Protocol for Visualization of Oomycetes In Vitro and In Planta.- Rapid and Robust Monitoring of Phytophthora Infectivity through Detached Leaf Assays with Automated Image Analysis.- Quantification of Phytophthora palmivora Infection in Barley and Related Monocot Roots.- The Use of eDNA Metabarcoding to Detect and Identify Phytophthora in Water Samples.- A Method for the Separation of Phytophthora Oospores from Soil for DNA-Based Detection.- Annotation of RxLR Effectors in Oomycete Genomes.- Pinus radiata cDNA Library for the Screening of Phytophthora Effector Protein Targets in Yeast.- Immunoisolation of Plant Endosomal Vesicles to Explore Uptake of Pathogen Effector Proteins during Infection of Nicotiana benthamiana.- Fabrication of Lab-on-a-Chip Devices to Study the Forces Imparted by Growing Phytophthora Hyphae.- Application of Proteomic Methods in Oomycete Biology.- Heterologous Expression of Secreted Proteins from Phytophthora in Pichia pastoris Followed by Protein Purification by Immobilized Metal Ion Affinity (IMAC).