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E-raamat: Pichia Protocols

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This book focuses on recent developments of Pichia pastoris as a recombinant protein production system. Highlighted topics include a discussion on the use of fermentors to grow Pichia pastoris, information on the O- and N-linked glycosylation, methods for labeling Pichia pastoris expressed proteins for structural studies, and the introduction of mutations in Pichia pastoris genes by the methods of restriction enzyme-mediated integration (REMI). Each chapter presents cutting-edge and cornerstone protocols for utilizing P. pastoris as a model recomibinant protein production system. This volume fully updates and expands upon the first edition.

This book focuses on recent developments of Pichia pastoris as a recombinant protein production system. The volume fully updates and expands upon the first edition, and focuses primarily on information that has come to light since its original publication.

Arvustused

From the reviews of the second edition:









"P. pastoris has many superior traits. can grow to very high cell densities prior to switching on an inducible promoter (the AOX1 gene) for maximal recombinant protein production. The diversity of such proteins produced by expression strains of P. pastoris is quite amazing and this book describes detailed experimental protocols for a few of them (e.g. hookworm protein, botulinum toxin). This is a stand-alone book . a book that will prove valuable to yeast molecular geneticists and biotechnologists." (Graeme Walker, Microbiology Today, May, 2008)

Table of Contents
Chapter 1 - Introduction [ REVIEW CHAPTER]
Chapter 2 - Vectors and strains for expression [ REVIEW CHAPTER]
Chapter 3 - DNA-Mediated Transformation
Chapter 4 - Rational design and optimization of fed-batch and continuous fermentations
Chapter 5 - Overexpression of a hookworm secretory protein saturates secretory capacity
Chapter 6 - Initial processing and purification strategies
Chapter 7 - Rapid screening of chromatography resins for the purification of proteins
Chapter 8 - Characterization of O-linked saccharides on glycoproteins
Chapter 9 - Modification of the N-glycosylation pathway to produce homogeneous human-like glycans using GlycoSwitch plasmids
Chapter 10 - N-linked glycan characterization of heterologous proteins
Chapter 11 - Heavy labeling of recombinant proteins
Chapter 12 - Selenomethionine labeling of recombinant proteins
Chapter 13 - Deuterium labeling of proteins
Chapter 14 - Classical genetic analysis
Chapter 15 - Identification of pexophagy genes by Restriction Enzyme-Mediated Integration (REMI)
Chapter 16 - Characterization of protein-protein interactions
Chapter 17 - Localization of proteins and organelles using fluorescence microscopy
Chapter 18 - Fluorescence microscopy and thin-section electron microscopy
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Püsilink: https://www.kriso.ee/db/97815974545682e.html
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