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E-raamat: Plant Abiotic Stress Signaling

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  • Formaat: EPUB+DRM
  • Sari: Methods in Molecular Biology 2642
  • Ilmumisaeg: 21-Mar-2023
  • Kirjastus: Springer-Verlag New York Inc.
  • Keel: eng
  • ISBN-13: 9781071630440
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  • Formaat: EPUB+DRM
  • Sari: Methods in Molecular Biology 2642
  • Ilmumisaeg: 21-Mar-2023
  • Kirjastus: Springer-Verlag New York Inc.
  • Keel: eng
  • ISBN-13: 9781071630440
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This volume provides conceptual strategies and methodological know-how over a wide range of stress situations that can be used as stepping stones to unravel the intricacies of abiotic stress signaling networks in plants. Chapters guide readers through achievements and challenges in the field and through up-to-date protocols covering identification of novel processes, validation of hypothetical mechanisms, and further characterization of currently-known pathways. Written in the format of the highly successful Methods in Molecular Biology series, wet-lab chapters include an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.





 





Authoritative and cutting-edge, Plant Abiotic Stress Signaling aims to be a comprehensive and innovative guide for students and researchers seeking to understand plant molecular mechanisms at the interface with environmental constraints and climate change.
Interplay of Methodology and Conceptualization in Plant Abiotic Stress
Signaling.- Complexity of Abiotic Stress Stimuli: Mimicking Hypoxic
Conditions Experimentally on the Basis of Naturally Occurring
Environments.- Iron Availability and Homeostasis in Plants: a Review of
Responses, Adaptive Mechanisms and Signaling.- Method for Calcium Live
Imaging at Multi-Scales from Cellular to Organ Level in Arabidopsis
thaliana.- Functions of NO and H2S Signal Molecules against Plant Abiotic
Stress.- Review of Lipid Biomarkers and Signals of Photooxidative Stress in
Plants.- The Plant Metabolic Changes and the Physiological and Signalling
Functions in the Responses to Abiotic Stress.- 15N-labelling of Leaves
Combined with GC-MS Analysis as a Tool for Monitoring the Dynamics of
Nitrogen Incorporation into Amino Acids.- Computational Metabolomics to
Elucidate Molecular Signalling and Regulatory Mechanisms Associated with
Biostimulant-Mediated Growth Promotion and Abiotic Stress Tolerance in Crop
Plants.- Electrical Signaling and its Functions under Conditions of Abiotic
Stress: a Review of Methodological Approaches and Physiological
Implications.- Quantitative measurements of biochemical and molecular markers
of oxidative stress signaling and responses.- Fast identification of in vivo
protein phosphorylation events using transient expression in leaf mesophyll
protoplasts and Phos-tagTM SDS-PAGE.- Analysis of Plant L-Cysteine
Desulfhydrase (LCD) Isozymes by Non-Denaturing Polyacrylamide Gel
Electrophoresis.- Metabolite-Based Genome-Wide Association Studies of
Large-Scale Metabolome Analysis to Illustrate Alterations in the Metabolite
Landscape of Plants upon Responses to Stresses.- Deciphering Macromolecular
Interactions Involved in Abiotic Stress Signaling: a Review of Bioinformatics
Analysis.- Multi-Omics Data Integration in the Context of Plant Abiotic
Stress Signaling.- Protein-Protein Interactions in Abiotic Stress Signaling:
an Overview of Biochemical and Biophysical Methods of
Characterization.- Beyond the Primary Structure of Nucleic Acids: Potential
Roles of Epigenetics and Noncanonical Structures in the Regulations of Plant
Growth and Stress Responses.- Laser microdissection: A High-Precision
Approach to Isolate Specific Cell Types from Any Plant Species for Downstream
Molecular Analyses.- An Experimental Rhizobox System for the Integrative
Analysis of Root Development and Abiotic Stress Responses under Water Deficit
Conditions.- Live Whole-Plant Detection of Rapidly Accumulating Reactive
Oxygen Species Following Applied Stress in Arabidopsis thaliana.- Analysis of
Small Non-Coding RNAs as Signaling Intermediates of
Environmentally-Integrated Responses to Abiotic Stress.- Perspectives in
Plant Abiotic Stress Signaling.