E-raamat: Plant Gametogenesis: Methods and Protocols

Measurement of flower metabolite concentrations using Gas-Chromatography
Mass Spectrometry and High-Performance Liquid Chromatography Mass
Spectrometry.- Proteomic-, phosphoproteomic- and Acetylomic-based mass
spectrometry to identify tissue-specific protein complexes and
phosphorylation in plant gametogenesis.- Identifying small RNAs and analyzing
their association with gene expression using isolated Arabidopsis male
meiocytes.- Genotyping-by-sequencing to map meiotic crossovers in plants.-
Interrogating global chromatin interaction network by high-throughput
chromosome conformation capture (Hi-C) in plants.- Cytogenetic techniques for
analysing meiosis in hexaploidy bread wheat.- Super-resolution chromatin
visualisation by combined fluorescence in situ hybridisation and Structured
Illumination Microscopy in Solanum lycopersicum.- Application of Chemical
Inhibitors in Live Cell Imaging of Plant Meiosis Using Light Sheet
Fluorescence Microscopy.- Bimolecular Fluorescence Complementation to Test
for Protein-Protein Interactions and To Uncover Regulatory Mechanisms During
Gametogenesis.- Quantifying meiotic crossover recombination in Arabidopsis
lines expressing fluorescent reporters in seeds using SeedScoring pipeline
for CellProfile.- Generation of mutants by combined treatment of physical and
chemical mutagens in rice.- Generation of mutant plants by Gamma Ray exposure
and development of low-cost TILLING population in chickpea (Cicer arietinum
L.).- Efficient generation of CRISPR/Cas9-based mutants supported by
fluorescent seed selection in different Arabidopsis accessions.- Generation
of deletion lines in Allohexaploid Bread Wheat.- Post-transcriptional Gene
Silencing Using Virus-Induced Gene Silencing to Study Plant Gametogenesis in
Tomato.- Protocols for in vivo doubled haploid (DH) technology in maize
breeding: From haploid inducer Development to Haploid Genome
Doubling.- Estimation of heterografting associated DNA methylation changes in
tree crops by MSAP analysis.- In vitro fertilization system using wheat
gametes by electric fusion.- High-throughput fluorescent pollen tetrad
analysis using DeepTetrad.- Barley (Hordeum vulgare) anther and meiocyte RNA
Sequencing: Mapping Sequencing reads and downstream data analyses.- Profiling
m6A RNA modifications in low amounts of plant cells using Maize
Meiocytes.- Assembling Plant Genomes with Long-Read
Sequencing.- Mapping-by-sequencing of point and insertional mutations with
Easymap.- Characterization of the different levels of variation in 45S rRNA
genes.