E-raamat: Plant Genome Engineering: Methods and Protocols

PAM-Less CRISPR-SpRY Genome Editing in Plants.- Type I-D CRISPR
System-Mediated Genome Editing in Plants.- CRISPR/LbCas12a-Mediated Genome
Editing in Soybean.- Base Editing in Poplar through an Agrobacterium-Mediated
Transformation Method.- Genetic Engineering of Potato (Solanum tuberosum)
Chloroplasts using the Small Synthetic Plastome
Mini-Synplastome.- Designing Guide-RNA for Generating Premature Stop Codons
for Gene Knockout using CRISPR-BETS.- Construction of CRISPR/Cas9 Multiplex
Genome Editing System in Rice.- Use of Fluorescent Protein Reporters for
Assessing and Detecting Genome Editing Reagents and Transgene Expression in
Plants.- Automated, High-Throughput Protoplast Transfection for Gene Editing
and Transgene Expression Studies.- Delivery of CRISPR-Cas12a
Ribonucleoprotein Complex for Genome Editing in an Embryogenic Citrus Cell
Line.- Transgene-Free Genome Editing in Nicotiana benthamiana with
CRISPR/Cas9 Delivered by a Rhabdovirus Vector .- Ribonucleoprotein
(RNP)-Mediated Targeted Mutagenesis in Barley (Hordeum vulgare
L.).- Ribonucleoprotein (RNP)-Mediated Allele Replacement in Barley (Hordeum
vulgare L.) Leaves.- Genome Editing in Chlamydomonas reinhardtii Using
Cas9-gRNA Ribonucleoprotein Complex: A Step-by-Step Guide.- Highly Efficient
Gene Knockout in Medicago truncatula Genotype R108 using CRISPR-Cas9 System
and an Optimized Agrobacterium Transformation Method.- Efficient Targeted
Mutagenesis in Brassica Crops using CRISPR/Cas Systems.- Introduction of
Genome Editing Reagents and Genotyping of Derived Edited Alleles in Soybean
(Glycine max (L.) Merr.).- A CRISPR/Cas9 Protocol for Target Gene Editing in
Barley.- Targeted Insertion in Nicotiana benthamiana Genomes via Protoplast
Regeneration.- Stepwise Optimization of Real-Time RT-PCR
Analysis.- CRISPR/Cas9 Technology for Potato Functional Genomics and
Breeding.- Recent Advances in Engineering of In Vivo Haploid Induction
Systems.