This volume provides lab-oriented protocols to deal the various plant microbiome engineering approaches in a lucid and simple manner. Chapters are divided into four section detailing plant associated microbiomes, single cell genomics, whole community metagenomics, metabolic network monitoring and advanced methods in plant microbiome engineering. Written in the format of the Methods and Protocols in Food Science series, the chapters include an introduction to the respective topic, list necessary materials and reagents, detail well-established and validated methods for readily reproducible laboratory protocols and contain notes on how to avoid or solve typical problems.
Authoritative and cutting-edge, Plant Microbiome Engineering aims to provide well-established protocols and procedures largely used by both academics and industrials.
Collection and processing of soil, rhizosphere, root tissue from crop
field and forests.- Collection and processing of bacteria from plant Vascular
tissues.- Collection and processing of plant fungal endophytes from shrubs.-
Collection And Processing Of Microalgae From Coralloid Roots Of Cycads.-
Collection and processing of plant endophytes from large woody plants.-
Collection and processing of halotolerant plant growth-promoting bacteria
from plant roots and adjacent areas.- Detection of Plant Growth Promoting
Bacteria from Soil.- Detection of plant growth promoting cyanobacterial
community from soil and root.- Detection of plant growth promoting
actinobacterial community from soil.- Detection of plant growth promoting
fungal community from soil.- Detection of microalgal community from soil and
plant root.- Determine the Symbiotic nitrogen fixation bacterial community
structure in legume crops.- Determine the Symbiotic nitrogen fixation
bacterial community structure in Casuarina sp..- Detection of endophytic
bacterial community structure in medicinal plant.- Rapid Detection of Plant
Associated Bacteria by MALDI-TOF Mass Spectrometry.- Rapid Detection of
Plant-associated Fungi by MALDI-TOF Mass Spectrometry.- Rapid detection of
plant-associated microalgae by MALDI-TOF mass spectrometry.- Nucleic
Acid-based Rapid Detection of Plant-associated Cyanobacteria.- Detection of
plant associated fungi by nucleic acid based technique.- Nucleic Acid-Based
Rapid Detection of Microalgae association in Plant.- Isolation of Genomic DNA
from plant growth promoting Pseudomonas species.- Isolation of genomic DNA
from plant growth promoting Streptomyces sp..- Isolation of Genomic DNA from
Plant Growth Promoting Cyanobacteria.- ISOLATION OF GENOMIC DNA FROM
MICROALGAE.- Separation of genomic DNA by agarose gel electrophoresis.-
Isolation of metagenomic DNA from rhizosphere soil.- Isolation of metagenomic
DNA from plant roots.- isolation of metagenomic DNA from root nodules.-
Quantification of metagenomic DNA.- COLONY PCR FOR RAPID DETECTION AND
IDENTIFICATION OF MICROALGAE.- Quantitative PCR for monitoring the plant
growth promoting microalgae from rice field.- Multivariate Analysis of Plant
microbiome diversity using Paleontological Statistics (PAST).- DNA
extraction, amplification and high-throughput sequencing of the 18S and ITS
regions of endophytic fungi.- Nextera DNA flex library preparation.- Illumina
Next Generation Sequencing of Metagenome.- Alpha diversity analysis using
Shannon index for each species in the rhizosphere soil.- Microbiome analyses
of Metagenome using USEARCH.- 16S rRNA microbiome analysis using QIIME.-
Microbiome Analysis of Metagenome using R studio.- Omics tools in plant
microbiome interactions.- Inference and Analysis of SPIEC-EASI Microbiome
Networks.- Rhizosphere meta transcriptomics.- Phyto microbiomes engineering
using clustered regularly interspaced short palindromic repeats (CRISPR).-
Transformation techniques for engineering microbiome bacterial species.-
Conjugation techniques for engineering microbiome bacterial species.- Plant
growth promoting functional analysis of Whole metagenome sequence of
bacteria.- Plant growth promoting functional analysis of Whole metagenome
sequence of Actinobacteria.- Plant growth promoting functional analysis of
Whole metagenome sequence of fungi.- In Situ Phyto microbiome Engineering.-
Inoculation of microorganisms into the soil and rhizosphere.- Inoculation of
microbes into seeds/ seedlings.- Direct injection of microbes into tissues /
wounds.- Microbiome Engineering for Plant Growth and Improvement.-
Phyto-Microbiome Engineering For Crop Growth And Resilience.- Plant
Microbiome Engineering to improve plant growth promotion and biocontrol.-
Microbiome engineering in Chromium Phytoremediation.- Measurement of
Arsenic-Tolerance Index of Fungal Strains as an Approach to Mitigating
Arsenic toxicity in Sustainable Agriculture.- Plant Microbiome Engineering to
improve Stress tolerance.- Plant Microbiome Engineering to improve Drought
Stress tolerance.- Genome Engineering Of Chlorella Sp..- Genome Engineering
of Frankia sp..- Bacillus genome engineering using Clustered Regularly
Interspaced Short Palindromic Repeats (CRISPR).
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