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E-raamat: Proteoglycans: Methods and Protocols

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Proteoglycans: Methods and ProtocolsSuurem pilt
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This volume is a comprehensive and up-to-date collection of strategies, reproducible methods, and protocols for the in-depth analysis of Proteoglycans (PGs) and their glycan part, the GAGs. Chapters are divided into three parts detailing GAGs in biological specimens, protocols for the evaluation of the in vitro and in vivo effects of PGs/GAGs, and protocols for compounds related with the metabolic enzymes, epigenetic regulation, and PGs/GAGs-based inhibitors. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Cutting-edge and thorough, Proteoglycans: Methods and Protocols aims to provide information on the elucidated the structural and functional aspects of the complex matrix macromolecules such as the proteoglycans and glycosaminoglycans.

 

Detection of Glycosaminoglycans in Biological Specimens.- Immunochemical
Detection and Glycosaminoglycan Disaccharide-Based Characterization Of
Chondroitin Sulfate Proteoglycans.- Proteoglycans: A Tool for detecting
hyaluronan by ELISA-like Methods.- Particle Exclusion Assay: A Tool for
Measuring Hyaluronan Pericellular Matrix.- Urinary Glycosaminoglycans:
Characterization and Quantification.- HPLC-MS characterization of
tissue-derived heparan sulfate and chondroitin sulfate.- Evaluation of
Glycosaminoglycans Biological Age in Cells and Tissues.- Studying the effects
of Glycosaminoglycans in Cell Morphological Aspect With Scanning Electron
Microscopy.- Evaluation of the In Vitro and In Vivo Effects of Biglycan in
Innate Immunity.- Analysis of Extracellular Vesicle-associated
Proteoglycans.- Analysis of Aggrecan Glycanation by Western Blot in Cell
Culture.- Repulsive Scaling Replica Exchange Molecular Dynamics in
ModelingProtein-Glycosaminoglycan Complexes.- In vitro investigation of
hyaluronan/CD44 network.- Separation and Identification of Native
Proteoglycans by Composite Agarose-Polyacrylamide Gel Electrophoresis and
Immunoblotting.- Building, Visualizing, and Analyzing
Glycosaminoglycan-Protein Interaction Networks.- An Enzymatic Activity Assay
for Heparanase That Is Useful for Evaluating Clinically Relevant Inhibitors
and Studying Kinetics.- A method for Urinary Trypsin Inhibitor (UTI)
purification combining anion-exchange chromatography enrichment and
preparative SDS-PAGE.- Enzymatic Sequencing of Heparin Oligosaccharides Using
Exolyase.- Evaluating the Effects of microRNAs on Proteoglycans and Matrix
Constituents Expression and Functional Properties.- Proteoglycan expression
studied by microRNAs.- Modeling and simulations of Extra Cellular
Glycoproteins.
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