| Preface |
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| Contributors |
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xiii | |
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Part I In Vitro Models of the Hepatocyte Life Cycle |
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1 Isolation and Culture of Mouse Hepatocytes: Gender-Specific Gene Expression Responses to Chemical Treatments |
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3 | (10) |
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2 Cryopreservation of Hepatocytes |
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13 | (14) |
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3 Culture Conditions Promoting Hepatocyte Proliferation and Cell Cycle Synchronization |
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27 | (26) |
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4 Immortalized Human Hepatic Cell Lines for In Vitro Testing and Research Purposes |
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53 | (24) |
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5 Culture and Functional Characterization of Human Hepatoma HepG2 Cells |
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77 | (18) |
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6 Establishment and Characterization of an In Vitro Model of Fas-Mediated Hepatocyte Cell Death |
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95 | (10) |
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7 Serum-Free Directed Differentiation of Human Embryonic Stem Cells to Hepatocytes |
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105 | (8) |
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Baltasar Lucendo-Villarin |
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8 Human Skin-Derived Precursor Cells: Isolation, Expansion, and Hepatic Differentiation |
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113 | (10) |
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9 Generation of Hepatocytes from Pluripotent Stem Cells for Drug Screening and Developmental Modeling |
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123 | (20) |
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10 Differentiation-Promoting Medium Additives for Hepatocyte Cultivation and Cryopreservation |
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143 | (18) |
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11 Coculture and Long-Term Maintenance of Hepatocytes |
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161 | (14) |
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12 Primary Hepatocytes in Sandwich Culture |
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175 | (14) |
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13 Establishing Liver Bioreactors for In Vitro Research |
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189 | (14) |
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14 Epigenetic Modifications as Antidedifferentiation Strategy for Primary Hepatocytes in Culture |
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203 | (10) |
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15 Transfection of Primary Hepatocytes with Liver-Enriched Transcription Factors Using Adenoviral Vectors |
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213 | (12) |
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Part II In Vitro Methods to Probe Hepatocyte Functionality and Toxicity |
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16 Transcriptomics of Hepatocytes Treated with Toxicants for Investigating Molecular Mechanisms Underlying Hepatotoxicity |
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225 | (16) |
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17 Global MicroRNA Analysis in Primary Hepatocyte Cultures |
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241 | (10) |
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18 Mass Spectrometry-Based Proteomics for Relative Protein Quantification and Biomarker Identification in Primary Human Hepatocytes |
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251 | (16) |
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19 Targeted Metabolomics for Homocysteine-Related Metabolites in Primary Hepatocytes |
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267 | (12) |
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20 Measurement of Cytochrome P450 Enzyme Induction and Inhibition in Human Hepatoma Cells |
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279 | (8) |
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21 Analysis of Sinusoidal Drug Uptake Transporter Activities in Primary Human Hepatocytes |
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287 | (16) |
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22 Measurement of Albumin Secretion as Functionality Test in Primary Hepatocyte Cultures |
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303 | (6) |
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23 Measurement of Blood Coagulation Factor Synthesis in Cultures of Human Hepatocytes |
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309 | (8) |
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24 Functionality Testing of Primary Hepatocytes in Culture by Measuring Urea Synthesis |
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317 | (6) |
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25 Assay of Bile Acid Conjugation and Excretion in Human Hepatocytes |
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323 | (10) |
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26 General Cytotoxicity Assessment by Means of the MTT Assay |
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333 | (16) |
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27 Measurement of Apoptotic and Necrotic Cell Death in Primary Hepatocyte Cultures |
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349 | (14) |
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28 Critical Factors in the Assessment of Cholestatic Liver Injury In Vitro |
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363 | (14) |
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29 In Vitro Cell Culture Models of Hepatic Steatosis |
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377 | (14) |
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30 Assessment of Liver Fibrotic Insults In Vitro |
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391 | (12) |
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| Index |
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403 | |
Lisainfo e-raamatute kohta