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E-raamat: Pulsed Electrical Discharges for Medicine and Biology: Techniques, Processes, Applications

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This book presents the application of pulsed electrical discharges in water and water dispersions of metal nanoparticles in medicine (surgery, dentistry, and oncology), biology and ecology. The intensive electrical and shock waves represent a novel technique to destroy viruses and this way to prepare anti-virus vaccines. The method of pulsed electrical discharges in water allows to decontaminate water from almost all known bacteria and spores of fungi being present in human beings. The nanoparticles used are not genotoxic and mutagenic. This book is useful for researchers and graduate students.

1 Introduction
1(6)
References
4(3)
2 Experimental Facility
7(16)
2.1 Test Bench of Electrodischarge Installations
7(1)
2.2 Types of Pulsed High-Voltage Generators
8(2)
2.3 High Efficiency Two-Channel Generator
10(3)
2.4 Portable Generator
13(1)
2.5 Electrodischarge Chambers
14(2)
2.6 Pilot Industrial Electrodischarge Apparatus
16(7)
References
22(1)
3 Diagnostic Techniques
23(10)
3.1 Electrical Measurements
23(1)
3.2 Optical Registration
24(3)
3.3 Physical and Chemical Measurements
27(3)
3.4 Biological and Medical Tests
30(3)
References
32(1)
4 Electrophysical Processes in Water
33(34)
4.1 Parameters and Properties of Electric Discharges
33(8)
4.2 Main Characteristics of Pulsed Electric Discharges in Water
41(4)
4.3 Shock Waves, Cavitation, and UV-Irradiation
45(10)
4.4 Impacts of the PED on Water Media
55(12)
References
63(4)
5 Substances Formed During Discharges
67(14)
5.1 Short-Lived Substances
68(2)
5.2 Nanoparticles
70(5)
5.3 Preliminary Results
75(6)
References
77(4)
6 Water Dispersions of Nanoparticles
81(40)
6.1 Electrothermal Erosion of Electrodes
81(6)
6.2 Parameters of Nanoparticles
87(3)
6.3 Distribution of Nanoparticles in Size
90(8)
6.4 Properties of Nanoparticles and the WDN
98(2)
6.5 Nanoparticles and Electric Conductivity of the WDN
100(3)
6.6 Chemical Properties of Nanoparticles
103(4)
6.7 Physical Properties of the WDN
107(2)
6.8 Study of the WDN by Physical and Chemical Methods
109(7)
6.9 Biological Properties of Nanoparticles
116(5)
References
118(3)
7 Impact of the WDN on Bacteria and Spores of Fungi
121(20)
7.1 Impact of the WDN on Bacteria
121(7)
7.2 Fungistatic Action of the WDN
128(5)
7.3 Prolonged Microbial Resistance of Water Treated by the PED
133(3)
7.4 Simulations of Microbes' Death in the WDN
136(5)
References
138(3)
8 Interaction of the WDN with Biological Objects
141(16)
8.1 Interaction of the WDN with Blood Serum
141(5)
8.2 Interaction of the WDN with Lysozyme
146(2)
8.3 Interaction of the Magnetic Nanoparticles and Yeasts' Cells
148(9)
References
155(2)
9 WDN and Living Beings
157(26)
9.1 Impact of the WDN on Biochemical Systems and Internal Organs
157(9)
9.2 Impact of the WDN on Anti-oxidant Protection
166(6)
9.2.1 Determination of Protein Thiol Groups by Amperometric Titration
167(1)
9.2.2 Determination of Malondialdehyde
168(1)
9.2.3 Determination of Reduced Glutathione
168(1)
9.2.4 Chemiluminescent Determination of Total Antioxidant Activity
169(1)
9.2.5 Determination of Activity of Superoxide Dismutase (ASOD) with Quercetin Autooxidation Reaction
169(1)
9.2.6 Determination of Plasma Ability to the H2O2 Splitting
170(2)
9.3 Impact of the WDN on Endocrine System and Intestinal Microflora
172(2)
9.4 Impact of the WDN on Immune System
174(4)
9.5 WDN and Genotoxicity
178(3)
9.6 Impact of the WDN on Crayfishes
181(2)
References
182(1)
10 Application of the PED and WDN
183(28)
10.1 Biology
183(4)
10.2 Surgery
187(6)
10.3 Oncology
193(3)
10.4 Stomatology
196(5)
10.5 Virology
201(4)
10.6 Ecology
205(6)
10.6.1 Test Run
205(4)
References
209(2)
Index 211