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E-raamat: Regulatory T-Cells: Methods and Protocols

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  • Formaat: PDF+DRM
  • Sari: Methods in Molecular Biology 2559
  • Ilmumisaeg: 30-Sep-2022
  • Kirjastus: Springer-Verlag New York Inc.
  • Keel: eng
  • ISBN-13: 9781071626474
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Regulatory T-Cells: Methods and ProtocolsSuurem pilt
  • Formaat: PDF+DRM
  • Sari: Methods in Molecular Biology 2559
  • Ilmumisaeg: 30-Sep-2022
  • Kirjastus: Springer-Verlag New York Inc.
  • Keel: eng
  • ISBN-13: 9781071626474
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This volume provides methods and models for investigating the immunosuppressive subset of CD4+ T-cells, regulatory T-cells (Treg). Chapters guide readers through, basic protocols for analyzing Treg in mice and humans, single-cell analysis for analyzing Treg, and methods for the analysis of Treg in clinical application. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.





 





Authoritative and cutting-edge, Regulatory T-Cells: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. 
Analysis of the in vivo function of follicular regulatory T (TFR) cells
in the regulation of antibody response.- Analysis of peripherally derived
Treg in the intestines.- Isolation and analysis of tumor infiltrating Treg.-
Induction of peripheral Treg.- Adoptive transfer and bone marrow chimera
models to analyse Treg function and differentiation.- Functional analysis of
Foxp3 and its mutants by retroviral transduction of murine primary CD4+ T
cells.- Genetic Tools for Analyzing Foxp3+ Treg cells: Fluorochrome-Based
Transcriptional Reporters and Genetic Fate-Mapping.- Depletion of Treg by the
Diphtheria Toxin System.- Analysis of FOXP3 DNA methylation patterns to
identify functional FOXP3+ T-Cell subpopulations.- Protein Ubiquitination
Assay.- Phenotypic and Functional Studies of Human Treg cell
Subpopulations.- In vitro analysis of CTLA-4 mediated transendocytosis by
Regulatory T cells.- Isolation and functional characterisation of regulatory
CD4+ Tcells from the inflamed joints of patients with rheumatoid arthritis.-
A GMP protocol for the manufacture of regulatory T cells for clinical
application.- Regulatory T-cell phenotyping using CyTOF.- High-dimensional
single-cell profiling of tumor-infiltrating CD4+ regulatory T cells.-
Single-cell transcriptome analysis of Treg.
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