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E-raamat: SARS-CoV-2: Methods and Protocols

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SARS-CoV-2: Methods and ProtocolsSuurem pilt
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This detailed volume provides the increasing number of SARS-CoV-2 researchers with a useful handbook covering multidisciplinary approaches on various aspects of SARS-CoV-2 research, brought together by leading laboratories across the globe. Topics covered include techniques in clinical and diagnostic virology, basic protocols in cell and virus culture, as well as bioinformatics and proteomics approaches in cellular response studies. This comprehensive collection also covers methods in immunology, animal models, antivirals and vaccine development strategies, as well as biorisk and mitigation measurements for SARS-CoV-2 research. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice that is vital for success in the lab. 

Practical and timely, SARS-CoV-2: Methods and Protocols serves as an ideal guide for scientists investigating this prevalent and perilous RNA virus and the novel coronavirus disease that results from it.
Evolution and Epidemiology of SARS-CoV-2 Virus.- Molecular Epidemiology
of SARS-CoV-2 by Sequencing.- Advanced Genetic Methodologies in Tracking
Evolution and Spread of SARS-CoV-2.- Antigen-Based Point of Care Testing
(POCT) for Diagnosing SARS-CoV-2:  Assessing Performance.- Diagnostic Method
for COVID-19 Using Sugar Chain-Immobilized Nanoparticles and Saliva
Specimens.- Detection and Quantification of SARS-CoV-2 by Real-Time RT-PCR
Assay.- Immunofluorescent Antibody Techniques in the Diagnosis of SARS-CoV-2
Infection in Humans.- Propagation and Quantification of SARS-CoV-2.-
Quantification of Infectious SARSCoV2 by the 50% Tissue Culture Infectious
Dose Endpoint Dilution Assay.- One-Step Reverse Transcription Droplet Digital
PCR Protocols for SARS-CoV-2 Detection and Quantification.- Profiling
SARS-CoV-2 Infection by High-Throughput Shotgun Proteomics.- Detection of
SARS-CoV-2 by Mass Spectrometry.- Bioinformatics-Based Approaches to Study
VirusHost InteractionsDuring SARS-CoV-2 Infection.- Human Nasal Epithelial
Cells (hNECs) Generated by Air-Liquid Interface (ALI) Culture as a Model
System for Studying the Pathogenesis of SARS-CoV-2.- Animal Models of
COVID-19: Nonhuman Primates.- Animal Models of COVID-19: Transgenic Mouse
Model.- Immunohistochemical Detection of SARS-CoV-2 Antigens, by Single and
Multiple Immunohistochemistry.- Measuring Neutralizing Antibodies to
SARS-CoV-2 Using Lentiviral Spike-Pseudoviruses.- Antiviral Strategies
Against SARS-CoV-2: A Systems Biology Approach.- Neutralization Assay for
SARS-CoV-2 Infection: Plaque Reduction Neutralization Test.-
Pseudovirus-Based Assays for the Measurement of Antibody-Mediated
Neutralization of SARS-CoV-2.- Cytopathic Effect (CPE)-Based Drug Screening
Assay for SARS-CoV-2.- Strengthening Biorisk Management in Research
Laboratories with Security-Sensitive Biological Agents like SARS-CoV-2.-
Biorisk Management for SARS-CoV-2 Research in a Biosafety Level-3 Core
Facility.- Methods of SARS-CoV-2 Inactivation.
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