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E-raamat: Signal Transduction Immunohistochemistry: Methods and Protocols

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  • Formaat: PDF+DRM
  • Sari: Methods in Molecular Biology 2593
  • Ilmumisaeg: 13-Dec-2022
  • Kirjastus: Springer-Verlag New York Inc.
  • Keel: eng
  • ISBN-13: 9781071628119
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Signal Transduction Immunohistochemistry: Methods and ProtocolsSuurem pilt
  • Formaat: PDF+DRM
  • Sari: Methods in Molecular Biology 2593
  • Ilmumisaeg: 13-Dec-2022
  • Kirjastus: Springer-Verlag New York Inc.
  • Keel: eng
  • ISBN-13: 9781071628119
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This new edition collects diverse protocols compiled by experts from different areas of research as well as by biotech researchers developing novel technologies in the area of immunohistochemistry (IHC). After a few chapters to help orient novices entering the biomedical arena, the book includes methods chapters covering multiplex IHC including fluorescence and chromogenic techniques, combination of IHC with In Situ Hybridization (ISH), wavelet transform approach for organelle tracking, transcription factors in human stem cells, differentiation of mesenchymal stem cells, 3D imaging, phenotyping of glial cells in the human brain, tissue fixation, permeabilization, and cryo-preservation, as well as other topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Signal Transduction Immunohistochemistry: Methods and Protocols, Third Edition aims to arm novices and experts with vital protocols they can use either as-is or tailor them for specific experimental needs.
Top 5 Considerations When Choosing an Antibody.- Validating Antibody
Specificities for Immunohistochemistry by Protein Blotting.- Recent Advances
in Chromogens for Immunohistochemistry.- Overview of Imaging Techniques in
Immunohistochemistry.- Visualizing Activated Myofibroblasts Resulting from
Differentiation of Mesenchymal Stem Cells.- Clinical Applications of Flow
Cytometry in Cancer Immunotherapies: From Diagnosis to Treatments.- Labeling
of Phospho-Specific Antibodies with oYo-Link® Epitope Tags for Multiplex
Immunostaining.- Multiplex Immunochromogenic Tissue Staining Employing
Primary Antibodies from the Same Species.- 3D Imaging for Cleared Tissues and
Thicker Samples on Confocal and Light-Sheet Microscopes.- Optical Tissue
Clearing Enables Three-Dimensional Morphometry in Experimental Nerve
Regeneration Research.- Protocol and Software for Automated Detection of
Lysosome Active Runs and Flights with Wavelet Transform Approach.-
Phenotyping of M1 and M2a Macrophages and Differential Expression of ACE-2 on
Monocytes by Flow Cytometry: Impact of Cell Culture Conditions and Sample
Processing.- In Situ Hybridization (ISH) Combined with Immunocytochemistry
(ICC) Co-Detection of Phosphorylated EGFR in A431 Cultured Cells.- In Situ
Hybridization (ISH) Combined with Immunohistochemistry (IHC) for Co-Detection
of EGFR RNA and Phosphorylated EGFR Protein in Lung Cancer Tissue.- Multiplex
Spatial Protein Detection by Combining Immunofluorescence with
Immunohistochemistry.- Transcription Factor-Mediated Differentiation of Motor
Neurons from Human Pluripotent Stem Cells.- Immunohistochemical Techniques
for Phosphoproteins.- Visualizing Cofilin-Actin Filaments by
Immunofluorescence and CryoEM: Essential Steps for Observing Cofilactin in
Cells.- Cyclic Multiplex Fluorescent Immunohistochemistry Protocol to
Phenotype Glial Cells in Human Formalin-Fixed Paraffin-Embedded Brain
Sections.- Spatially Characterizing the Immune Contexture in Mouse Tissue
Using Multiplex Immunohistochemistry.- Highly Multiplexed Immuno-Fluorescence
Imaging for Quantitative Spatial Analysis in Tissue Samples with
ChipCytometryTM.
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