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Chapter 1 Design of In Situ Cytosensing Strategies |
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1 | (20) |
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1.1 General Principles of In Situ Cytosensing |
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1 | (1) |
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1.2 Enhancement of Detection Specificity |
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2 | (2) |
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1.3 Design of Off-On Signal Switch |
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4 | (3) |
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1.4 Design of Signal Amplification Strategy |
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7 | (6) |
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1.5 Design of Multi-channel Analysis |
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13 | (4) |
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17 | (1) |
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18 | (3) |
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Chapter 2 In Situ Detection of Cell-surface Glycans |
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21 | (26) |
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2.1 Introduction to Cell-surface Glycans |
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21 | (1) |
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2.1.1 Structures and Classification of Glycans |
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21 | (1) |
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2.1.2 Functions of Glycans |
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22 | (1) |
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2.2 Labelling and Recognition of Glycans |
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22 | (5) |
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22 | (2) |
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2.2.2 Chemoselective Labelling |
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24 | (1) |
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2.2.3 Metabolic Oligosaccharide Engineering |
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25 | (2) |
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2.2.4 Chemoenzymatic Glycan Labelling |
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27 | (1) |
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2.3 Global Analysis of Cell-surface Glycans |
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27 | (6) |
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2.4 Competition Systems for Quantification of Cell-surface Glycans |
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33 | (2) |
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2.5 Protein-specific Imaging of Cell-surface glycans |
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35 | (8) |
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43 | (1) |
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44 | (3) |
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Chapter 3 In Situ Detection of Intracellular Messenger RNA and MicroRNA |
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47 | (20) |
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48 | (1) |
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3.2 Biofunction of mRNA and miRNA |
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48 | (2) |
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3.2.1 Biofunction of mRNA |
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49 | (1) |
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3.2.2 Biofunction of miRNA |
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50 | (1) |
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50 | (3) |
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3.3.1 LentWRetiovirus-mediated Vectors for Gene Delivery |
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51 | (1) |
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3.3.2 Adenovirus-mediated Vectors for Gene Delivery |
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51 | (2) |
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3.3.3 Adeno-associated Virus Vectors for Gene Delivery |
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53 | (1) |
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53 | (2) |
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54 | (1) |
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55 | (1) |
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3.5 Inorganic Gene Vectors |
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55 | (2) |
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3.5.1 Gold-based Nanomaterials as Gene Vectors |
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55 | (1) |
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3.5.2 Two-dimensional GO and TMD Nanosheets as Gene Vectors |
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56 | (1) |
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3.6 Intracellular mRNA Detection |
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57 | (2) |
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3.7 Intracellular miRNA Detection |
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59 | (2) |
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3.7.1 "One-to-One" Signal Detection |
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59 | (1) |
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3.7.2 Sensitive Detection Based on Signal Amplification |
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59 | (2) |
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3.8 Perspective and Challenges |
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61 | (1) |
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61 | (1) |
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61 | (6) |
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Chapter 4 In Situ Analysis of Intracellular Telomerase Activity |
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67 | (15) |
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4.1 Clinical Significance of Telomerase |
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67 | (2) |
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4.2 Traditional Analytical Methods for Telomerase |
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69 | (1) |
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4.3 M Situ Imaging of Intracellular Telomerase Activity Based on Functional Nanoprobes |
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70 | (2) |
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4.4 In Situ Imaging of Intracellular Telomerase Activity Through FRET |
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72 | (4) |
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4.4.1 FRET Based on Organic Quenchers |
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73 | (1) |
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4.4.2 FRET Based on AuNPs |
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73 | (3) |
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4.5 In Situ Monitoring of Intracellular Telomerase Activity Sensing |
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76 | (2) |
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4.6 In Situ Monitoring of Intracellular Telomerase Activity Using AIEgen-based Bioprobes |
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78 | (1) |
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79 | (1) |
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79 | (1) |
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80 | (2) |
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Chapter 5 In Situ Analysis of the Intracellular Caspase Family |
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82 | (30) |
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5.1 Introduction to the Intracellular Caspase Family |
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82 | (1) |
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5.2 Imaging of Caspase Activity in Living Cells |
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83 | (18) |
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5.2.1 FRET-based Sensors for Imaging of Intracellular Caspase Activity |
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83 | (4) |
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5.2.2 Fluorescence Lifetime Imaging Microscopy for Imaging of Intracellular Caspase Activity |
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87 | (3) |
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5.2.3 AIE Probe for Imaging of Intracellular Caspase Activity |
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90 | (2) |
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5.2.4 Two-photon (TP) Imaging of Intracellular Caspase Activity |
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92 | (2) |
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5.2.5 Nanomaterial-based Probe for Imaging of Intracellular Caspase Activity |
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94 | (3) |
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5.2.6 Bioorthogonal Cyclization-mediated In Situ Self-assembly of Small-molecule Probes for Imaging of Caspase Activity |
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97 | (2) |
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5.2.7 Bioluminescence Imaging of Intracellular Caspase Activity |
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99 | (2) |
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5.3 Simultaneous Imaging of Caspases and Other Proteins |
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101 | (3) |
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5.4 Real-time Monitoring of Caspase Cascade Activation |
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104 | (4) |
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108 | (1) |
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108 | (1) |
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108 | (4) |
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Chapter 6 Visualization of Intracellular Glycosylation |
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112 | (32) |
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6.1 Introduction to Intracellular Glycosylation |
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112 | (2) |
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112 | (1) |
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113 | (1) |
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6.2 Visualization of Intracellular O-GlcNAcylation |
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114 | (11) |
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6.2.1 Protein-specific O-GlcNAcylation |
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114 | (5) |
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6.2.2 General O-GlcNAcylation |
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119 | (6) |
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6.3 Visualization of Intracellular Sialylation-related Enzymes |
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125 | (14) |
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125 | (8) |
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133 | (6) |
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139 | (1) |
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139 | (1) |
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139 | (5) |
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Chapter 7 Real-time Monitoring of Intracellular Reactive Oxygen Species |
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144 | (26) |
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7.1 Fluorescent Probes for Superoxide Anion |
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144 | (5) |
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7.1.1 Phenol-Quinone Interconversion |
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44 | (103) |
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7.1.2 Privileged Hydrogen Abstraction Reaction of Benzothiazoline |
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147 | (1) |
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7.1.3 Chemiluminescence Method |
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148 | (1) |
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7.1.4 Nucleophilic Substitution Reaction |
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149 | (1) |
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7.2 Fluorescent Probes for Hydrogen Peroxide |
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149 | (5) |
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7.3 Fluorescent Probes for Hydroxyl Radical |
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154 | (3) |
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7.4 Fluorescent Probes for Lipid Peroxy Radical |
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157 | (3) |
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7.5 Fluorescent Probes for Other Reactive Oxygen Species |
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160 | (6) |
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7.5.1 Fluorescent Probes for HClO |
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160 | (3) |
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7.5.2 Fluorescent Probes for HOBr |
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163 | (2) |
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7.5.3 Fluorescent Probes for 1O2 |
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165 | (1) |
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7.5.4 Fluorescent Probes for O3 |
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165 | (1) |
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166 | (1) |
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166 | (4) |
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Chapter 8 Imaging of Intracellular Reactive Nitrogen Species and Reactive Sulfur Species |
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170 | (41) |
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170 | (1) |
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8.2 Imaging of Intracellular Reactive Nitrogen Species |
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171 | (16) |
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8.2.1 Probes for Nitroxyl (HNO) |
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171 | (4) |
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8.2.2 Probes for Nitric Oxide (NO) |
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175 | (7) |
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8.2.3 Probes for Peroxynitrite (ONOO-) |
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182 | (5) |
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8.3 Fluorescent Probes for GSH, Cys and Hey |
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187 | (5) |
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8.3.1 Fluorescent Probes for GSH |
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187 | (2) |
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8.3.2 Fluorescent Probes for Cys |
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189 | (1) |
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8.3.3 Fluorescent Probes for Hey |
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190 | (1) |
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8.3.4 Fluorescent Probes for Two or All of GSH, Cys and Hey |
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191 | (1) |
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8.4 Fluorescent Probes for H2S |
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192 | (5) |
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8.4.1 Nucleophilic Addition of H2S |
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193 | (2) |
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8.4.2 H2S-induced Thiolysis |
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195 | (1) |
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8.4.3 Azide Group Reduction |
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196 | (1) |
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8.4.4 Other Assay Principles |
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197 | (1) |
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8.5 Fluorescent Probes for H2Sn |
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197 | (2) |
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8.6 Fluorescent Probes for Cys-SSH |
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199 | (1) |
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8.7 Fluorescent Probes for SO2 |
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199 | (3) |
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202 | (1) |
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202 | (9) |
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Chapter 9 Imaging of the Tumour Microenvironment |
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211 | (21) |
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211 | (1) |
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9.2 Fluorescent Probes for Intracellular pH and Hypoxia Imaging |
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212 | (3) |
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9.3 pH and Hypoxia Activatable Tumour Imaging |
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215 | (5) |
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9.4 Tumour pH and Hypoxia Targeting Nanotechnology |
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220 | (2) |
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9.5 pH and Hypoxia Imaging for Tumour Therapeutic Monitoring |
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222 | (5) |
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227 | (1) |
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228 | (1) |
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228 | (4) |
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Chapter 10 Visualization of Dynamic Intermolecular Interactions in Living Cells |
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232 | (16) |
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232 | (1) |
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10.2 Dynamic Interactions among Active Small Molecules |
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233 | (5) |
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10.2.1 Dual Detection of ROS |
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233 | (2) |
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10.2.2 Dual Detection of ROS and RSS |
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235 | (2) |
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10.2.3 Dual Detection of ROS and pH |
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237 | (1) |
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10.2.4 Dual Detection of ROS and MicroRNA |
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237 | (1) |
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10.2.5 Dual Detection of RNS and RSS |
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238 | (1) |
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10.3 Dynamic Interactions among Biomacromolecules |
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238 | (4) |
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239 | (1) |
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240 | (2) |
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242 | (1) |
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10.4 Dynamic Intermolecular Interactions Between Active Small Molecules and Biomacromolecules |
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242 | (2) |
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10.4.1 Dual Detection of Phosphatase and H2S |
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242 | (2) |
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10.4.2 Dual Detection of miRNA and mRNA |
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244 | (1) |
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244 | (1) |
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244 | (1) |
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245 | (3) |
Subject Index |
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248 | |