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E-raamat: In Situ Analysis of Cellular Functional Molecules

(Nanjing University, China), (Nanjing University, China), (Shandong Normal University, China)
  • Formaat: 254 pages
  • Sari: Detection Science Series Volume 16
  • Ilmumisaeg: 14-Jan-2020
  • Kirjastus: Royal Society of Chemistry
  • Keel: eng
  • ISBN-13: 9781839161353
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  • Formaat: 254 pages
  • Sari: Detection Science Series Volume 16
  • Ilmumisaeg: 14-Jan-2020
  • Kirjastus: Royal Society of Chemistry
  • Keel: eng
  • ISBN-13: 9781839161353

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This book introduces the tailor-made design of detection probes as well as schemes from a top-down perspective according to the unique characteristics of cellular functional molecules.

In situ analysis of cellular functional molecules has attracted considerable interest as it can provide spatially or temporally resolved information of these essential molecules on/within living cells through non-invasive methods. This book introduces the tailor-made design of detection probes as well as schemes from a top-down perspective according to the unique characteristics of cellular functional molecules. Written by leaders in the field, it will provide a comprehensive overview to those working on different aspects of cellular analysis and cell biology.

In recent years, in situ analysis of cellular functional molecules has attracted considerable interest as it can provide spatially or temporally resolved information of these essential molecules on/within living cells in a non-invasive way. In Situ Analysis of Cellular Functional Molecules introduces the tailor-made design of detection probes as well as schemes from a top-down perspective according to the unique characteristics of cellular functional molecules. The latest methodological developments, including enhancement of detection sensitivity and specificity, precision localization, implementation of dynamic tracking, and acquirement of quantitative functional information for various important functional molecules is discussed in detail. Written by leaders in the field, this book will provide a comprehensive overview to scientists in academia and professionals in industry working on different aspects of cellular analysis and cell biology. In situ analysis of cellular functional molecules has attracted considerable interest as it can provide spatially or temporally resolved information of these essential molecules on/within living cells through non-invasive methods. This book introduces the tailor-made design of detection probes as well as schemes from a top-down perspective according to the unique characteristics of cellular functional molecules. Written by leaders in the field, it will provide a comprehensive overview to those working on different aspects of cellular analysis and cell biology.
Chapter 1 Design of In Situ Cytosensing Strategies
1(20)
1.1 General Principles of In Situ Cytosensing
1(1)
1.2 Enhancement of Detection Specificity
2(2)
1.3 Design of Off-On Signal Switch
4(3)
1.4 Design of Signal Amplification Strategy
7(6)
1.5 Design of Multi-channel Analysis
13(4)
1.6 Perspective
17(1)
References
18(3)
Chapter 2 In Situ Detection of Cell-surface Glycans
21(26)
2.1 Introduction to Cell-surface Glycans
21(1)
2.1.1 Structures and Classification of Glycans
21(1)
2.1.2 Functions of Glycans
22(1)
2.2 Labelling and Recognition of Glycans
22(5)
2.2.1 Lectin Recognition
22(2)
2.2.2 Chemoselective Labelling
24(1)
2.2.3 Metabolic Oligosaccharide Engineering
25(2)
2.2.4 Chemoenzymatic Glycan Labelling
27(1)
2.3 Global Analysis of Cell-surface Glycans
27(6)
2.4 Competition Systems for Quantification of Cell-surface Glycans
33(2)
2.5 Protein-specific Imaging of Cell-surface glycans
35(8)
2.6 Perspective
43(1)
References
44(3)
Chapter 3 In Situ Detection of Intracellular Messenger RNA and MicroRNA
47(20)
3.1 Introduction
48(1)
3.2 Biofunction of mRNA and miRNA
48(2)
3.2.1 Biofunction of mRNA
49(1)
3.2.2 Biofunction of miRNA
50(1)
3.3 Viral Gene Vectors
50(3)
3.3.1 LentWRetiovirus-mediated Vectors for Gene Delivery
51(1)
3.3.2 Adenovirus-mediated Vectors for Gene Delivery
51(2)
3.3.3 Adeno-associated Virus Vectors for Gene Delivery
53(1)
3.4 Organic Gene Vectors
53(2)
3.4.1 Cationic Polymers
54(1)
3.4.2 Cationic Lipids
55(1)
3.5 Inorganic Gene Vectors
55(2)
3.5.1 Gold-based Nanomaterials as Gene Vectors
55(1)
3.5.2 Two-dimensional GO and TMD Nanosheets as Gene Vectors
56(1)
3.6 Intracellular mRNA Detection
57(2)
3.7 Intracellular miRNA Detection
59(2)
3.7.1 "One-to-One" Signal Detection
59(1)
3.7.2 Sensitive Detection Based on Signal Amplification
59(2)
3.8 Perspective and Challenges
61(1)
Acknowledgement
61(1)
References
61(6)
Chapter 4 In Situ Analysis of Intracellular Telomerase Activity
67(15)
4.1 Clinical Significance of Telomerase
67(2)
4.2 Traditional Analytical Methods for Telomerase
69(1)
4.3 M Situ Imaging of Intracellular Telomerase Activity Based on Functional Nanoprobes
70(2)
4.4 In Situ Imaging of Intracellular Telomerase Activity Through FRET
72(4)
4.4.1 FRET Based on Organic Quenchers
73(1)
4.4.2 FRET Based on AuNPs
73(3)
4.5 In Situ Monitoring of Intracellular Telomerase Activity Sensing
76(2)
4.6 In Situ Monitoring of Intracellular Telomerase Activity Using AIEgen-based Bioprobes
78(1)
4.7 Perspective
79(1)
Acknowledgement
79(1)
References
80(2)
Chapter 5 In Situ Analysis of the Intracellular Caspase Family
82(30)
5.1 Introduction to the Intracellular Caspase Family
82(1)
5.2 Imaging of Caspase Activity in Living Cells
83(18)
5.2.1 FRET-based Sensors for Imaging of Intracellular Caspase Activity
83(4)
5.2.2 Fluorescence Lifetime Imaging Microscopy for Imaging of Intracellular Caspase Activity
87(3)
5.2.3 AIE Probe for Imaging of Intracellular Caspase Activity
90(2)
5.2.4 Two-photon (TP) Imaging of Intracellular Caspase Activity
92(2)
5.2.5 Nanomaterial-based Probe for Imaging of Intracellular Caspase Activity
94(3)
5.2.6 Bioorthogonal Cyclization-mediated In Situ Self-assembly of Small-molecule Probes for Imaging of Caspase Activity
97(2)
5.2.7 Bioluminescence Imaging of Intracellular Caspase Activity
99(2)
5.3 Simultaneous Imaging of Caspases and Other Proteins
101(3)
5.4 Real-time Monitoring of Caspase Cascade Activation
104(4)
5.5 Perspective
108(1)
Acknowledgement
108(1)
References
108(4)
Chapter 6 Visualization of Intracellular Glycosylation
112(32)
6.1 Introduction to Intracellular Glycosylation
112(2)
6.1.1 O-GlcNAcylation
112(1)
6.1.2 Sialylation
113(1)
6.2 Visualization of Intracellular O-GlcNAcylation
114(11)
6.2.1 Protein-specific O-GlcNAcylation
114(5)
6.2.2 General O-GlcNAcylation
119(6)
6.3 Visualization of Intracellular Sialylation-related Enzymes
125(14)
6.3.1 Intracellular STs
125(8)
6.3.2 Intracellular Neus
133(6)
6.4 Perspective
139(1)
Acknowledgement
139(1)
References
139(5)
Chapter 7 Real-time Monitoring of Intracellular Reactive Oxygen Species
144(26)
7.1 Fluorescent Probes for Superoxide Anion
144(5)
7.1.1 Phenol-Quinone Interconversion
44(103)
7.1.2 Privileged Hydrogen Abstraction Reaction of Benzothiazoline
147(1)
7.1.3 Chemiluminescence Method
148(1)
7.1.4 Nucleophilic Substitution Reaction
149(1)
7.2 Fluorescent Probes for Hydrogen Peroxide
149(5)
7.3 Fluorescent Probes for Hydroxyl Radical
154(3)
7.4 Fluorescent Probes for Lipid Peroxy Radical
157(3)
7.5 Fluorescent Probes for Other Reactive Oxygen Species
160(6)
7.5.1 Fluorescent Probes for HClO
160(3)
7.5.2 Fluorescent Probes for HOBr
163(2)
7.5.3 Fluorescent Probes for 1O2
165(1)
7.5.4 Fluorescent Probes for O3
165(1)
Acknowledgement
166(1)
References
166(4)
Chapter 8 Imaging of Intracellular Reactive Nitrogen Species and Reactive Sulfur Species
170(41)
8.1 Introduction
170(1)
8.2 Imaging of Intracellular Reactive Nitrogen Species
171(16)
8.2.1 Probes for Nitroxyl (HNO)
171(4)
8.2.2 Probes for Nitric Oxide (NO)
175(7)
8.2.3 Probes for Peroxynitrite (ONOO-)
182(5)
8.3 Fluorescent Probes for GSH, Cys and Hey
187(5)
8.3.1 Fluorescent Probes for GSH
187(2)
8.3.2 Fluorescent Probes for Cys
189(1)
8.3.3 Fluorescent Probes for Hey
190(1)
8.3.4 Fluorescent Probes for Two or All of GSH, Cys and Hey
191(1)
8.4 Fluorescent Probes for H2S
192(5)
8.4.1 Nucleophilic Addition of H2S
193(2)
8.4.2 H2S-induced Thiolysis
195(1)
8.4.3 Azide Group Reduction
196(1)
8.4.4 Other Assay Principles
197(1)
8.5 Fluorescent Probes for H2Sn
197(2)
8.6 Fluorescent Probes for Cys-SSH
199(1)
8.7 Fluorescent Probes for SO2
199(3)
Acknowledgement
202(1)
References
202(9)
Chapter 9 Imaging of the Tumour Microenvironment
211(21)
9.1 Introduction
211(1)
9.2 Fluorescent Probes for Intracellular pH and Hypoxia Imaging
212(3)
9.3 pH and Hypoxia Activatable Tumour Imaging
215(5)
9.4 Tumour pH and Hypoxia Targeting Nanotechnology
220(2)
9.5 pH and Hypoxia Imaging for Tumour Therapeutic Monitoring
222(5)
9.6 Perspective
227(1)
Acknowledgement
228(1)
References
228(4)
Chapter 10 Visualization of Dynamic Intermolecular Interactions in Living Cells
232(16)
10.1 Introduction
232(1)
10.2 Dynamic Interactions among Active Small Molecules
233(5)
10.2.1 Dual Detection of ROS
233(2)
10.2.2 Dual Detection of ROS and RSS
235(2)
10.2.3 Dual Detection of ROS and pH
237(1)
10.2.4 Dual Detection of ROS and MicroRNA
237(1)
10.2.5 Dual Detection of RNS and RSS
238(1)
10.3 Dynamic Interactions among Biomacromolecules
238(4)
10.3.1 Proteins
239(1)
10.3.2 DNAs
240(2)
10.3.3 RNAs
242(1)
10.4 Dynamic Intermolecular Interactions Between Active Small Molecules and Biomacromolecules
242(2)
10.4.1 Dual Detection of Phosphatase and H2S
242(2)
10.4.2 Dual Detection of miRNA and mRNA
244(1)
10.5 Perspectives
244(1)
Acknowledgement
244(1)
References
245(3)
Subject Index 248
Prof. Huangxian Ju received his B.S., M.S. and Ph.D from Nanjing University in 1986, 1989 and 1992, and became lecturer, associate professor and professor in 1992, 1993 and 1999, respectively. He is currently the director of State Key Laboratory of Analytical Chemistry for Life Science. He won the National Funds for National Distinguished Young Scholars in 2003, and was selected as Changjiang Professor by Education Ministry of China and a National Key Talent in The New Century in 2007 by China government, a chief scientist of National Basic Research Program of China by MSTC in 2009, Fellows of the International Society of Electrochemistry and the Royal Society of Chemistry in 2015. His research interests include analytical biochemistry and nanobiosensing. He has published 569 papers in SCI journals (329 in journals with IF over 5), and authored 3 English books, 7 Chinese books, 17 chapters, and 6 editorial, preface or book reviews. His research works have 25584 citations in SCI journals with an h-index of 84 (Google Scholar h-index 93 with 30450 citations) up to Aug. 10, 2018, and won 3 first-class S&T prizes from Jiangsu Province Government, 3 first-class prizes in natural science from Education Ministry of China, and 3 first-class S&T prizes from Chinese Association for Instrumental Analysis.   Prof. Bo Tang received his Ph.D. degree from Nankai University in 1994. He currently works in Shandong Normal University as professor, president of Shandong Normal University. He is also the adjunct professor of Nankai University and Shandong University. Prof. Tang won National Fund for Outstanding Young Scientists in 2007, Chief-scientist for National key basic research development plan (973 Program) in 2012. He is director of Research Center of the Key Laboratory of Molecular and Nano Probes Ministry of Education in Shandong Normal University. Prof. Tang currently is Honorary Advisors of Analytical Chemistry and serves on the Editorial Board of Scientific Reports. His research interest is focused on the synthesis of molecular and nanoprobes and its application in biological imaging, synthesis of fluorescent material, solar chemical conversion and storage. Up to now, Prof. Tang has published over 400 papers in the journals of Nat. Commun., J. Am. Chem. Soc., Angew. Chem. Int. Ed., Chem. Sci., Biomaterials, and so on. His work has been cited over 12,000 times not counting self-citations and his current H-index is 58.



Prof. Lin Ding, graduated from Prof. Jus group with Ph.D degree in 2009, is now a professor of chemistry in Nanjing University. She is also the holder of Excellent Young Scientist Award from NSFC. Her research interests center on the in situ detection of cellular functional molecules, with 60 papers in SCI journals.