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E-raamat: Spermatogonial Stem Cells: Methods and Protocols

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  • Formaat: PDF+DRM
  • Sari: Methods in Molecular Biology 2656
  • Ilmumisaeg: 30-May-2023
  • Kirjastus: Springer-Verlag New York Inc.
  • Keel: eng
  • ISBN-13: 9781071631393
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Spermatogonial Stem Cells: Methods and ProtocolsSuurem pilt
  • Formaat: PDF+DRM
  • Sari: Methods in Molecular Biology 2656
  • Ilmumisaeg: 30-May-2023
  • Kirjastus: Springer-Verlag New York Inc.
  • Keel: eng
  • ISBN-13: 9781071631393
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This volume explores the latest techniques used in the field of mammalian germ cell biology to study key experimental approaches and perspectives for critical analysis of spermatogonial subsets including the rare spermatogonial stem cells (SSCs) pool.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding common pitfalls.

Cutting-edge and comprehensive, Spermatogonial Stem Cells: Methods and Protocols is a valuable resource for both new and experiences investigators looking to expand their knowledge in this developing field.  

Introduction: The Whys and Hows for Studying Spermatogenesis and
Spermatogonial Stem Cells.- Optimizing Testicular Fixation-Embedding
Procedures for an Improved Morphofunctional Evaluation of Mammalian
Spermatogonia.- Single-Molecule Fluorescence In Situ Hybridization for
Spatial Detection of mRNAs in Sections of Mammalian Testes.- Bulk and
Single-Cell RNA-Seq Analyses for Studies of Spermatogonia.- Profiling the
Epigenetic Landscape of the Spermatogonial Stem Cell Part 1: Epigenomics
Assays.- Profiling the Epigenetic Landscape of the Spermatogonial Stem Cell
Part 2: Computational Analysis of Epigenomics Data.- Primary Cultures of
Spermatogonia and Testis Cells.- Reconstitution of Human Prospermatogonial
Development from Human Induced Pluripotent Stem Cells.- RNA Interference as a
Method of Gene Knockdown in Cultured Spermatogonia.- Isolation of
Undifferentiated Spermatogonia from Adult and Developing Mouse
Testes.- Spermatogonial Transplantation.- Viral Transduction of Mammalian
Spermatogonial Stem Cells.- Pharmacological Treatment of Neonatal and
Juvenile Mice to Study Spermatogenesis.- Models and Methods for Evaluating
Regeneration of Spermatogenesis After Cytotoxic Treatments.- Defining Gene
Function in Spermatogonial Stem Cells through Conditional Knockout
Approaches.- Lineage Tracing of Spermatogonial Stem Cells within the Male
Germline.- Perspectives: Approaches for Studying Livestock
Spermatogonia.- Perspectives: Methods for Evaluating Primate Spermatogonial
Stem Cells.
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