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E-raamat: Standard Operational Procedures in Reproductive Medicine: Laboratory and Clinical Practice

Edited by (University of South Alabama, USA), Edited by (CEO, iLab Comm, St Augustin, Germany)
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Compiled by two experts in Reproductive Medicine, with contributions from internationally respected specialists, this innovative text lets the whole team in Reproductive Medicine get literally on the same page. Taking a cook-book approach to the operational procedures in the laboratory and in the clinic, it details what needs to be prepared in advance, what needs to be prepared earlier the same day, and what steps to take before, during, and after the procedure itself. This is an essential tool for ensuring all staff - whether experienced or starters - can be confident in their tasks and are in touch with what is expected of them and their colleagues.

About the Editors xi
List of Contributors
xiii
Section I Laboratory Procedures
1 The Documentation System
2(2)
Markus Montag
Lars Johansson
2 Culture Media Techniques
4(4)
Lars Johansson
3 IVF Incubator Handling
8(4)
Jason E. Swain
Alex Lagunov
4 Preparation for Follicle Aspiration and Isolation of Cumulus-Oocyte-Complexes (COC)
12(2)
Markus Montag
5 Semen Collection and Sample Reception
14(4)
Carin Huyser
6 Spermiogramm, Makler Chamber, Neubauer Chamber, CASA
18(2)
Verena Nordhoff
7 Sperm Viability Testing
20(2)
Markus Montag
8 Sperm Preparation---Swim Up, Density Gradient, Migration Chamber, Zeta Method
22(2)
Thomas Ebner
9 Sperm Selection Using Hyaluronan Binding
24(2)
Denny Sakkas
10 IVF---Insemination
26(2)
Markus Montag
11 ICSI
28(2)
Thomas Freour
12 Oocyte In Vitro Maturation---Isolation of COCs and Culture for Maturation
30(2)
Mariabeatrice Dal Canto
Giovanni Coticchio
13 Artificial Oocyte Activation
32(2)
Markus Montag
14 Cryopreservation---Sperm TESE and MESA
34(2)
Borut Kovacic
15 Vitrification---Oocytes, 2PN, Embryos, Blastocysts
36(2)
Ana Cobo
Aila Coello
16 Slow Freezing: Principles, Oocytes, 2PN, Embryos, Blastocysts
38(2)
Etienne Van den Abbeel
17 Oocyte Selection
40(2)
Basak Balaban
18 Zygote Selection
42(2)
Martin Greuner
19 Selection and Grading of Embryos on Day 2/3 and Blastocysts on Day 5/6
44(2)
Thorir Hardarson
Julius Hreinsson
20 Time-Lapse Imaging
46(4)
Cristina Hickman
21 Assisted Hatching
50(2)
Christian De Geyter
Maria De Geyter
22 Zona Manipulation at Various Embryo Stages: Mechanical/Acidic Medium
52(4)
Marius Meintjes
23 Biopsy: Polar Body, Blastomere, Trophectoderm
56(4)
Hakan Yelke
Semra Kahraman
24 Sampling of Cells after Biopsy for aCGH
60(2)
Barry Behr
25 Embryo Transfer---Standard and Ultrasound Guided
62(2)
Bettina Toth
26 Hygienic Standards in the IVF Laboratory
64(4)
Vera Baukloh
27 Quality Control of IVF Laboratory Supplies---Mouse Embryo Assay (MEA)
68(2)
Dean E. Morbeck
28 Troubleshooting in the IVF Laboratory
70(2)
David Mortimer
Sharon T. Mortimer
29 Microscopy
72(2)
Markus Montag
30 Fertility Preservation
74(4)
Markus Montag
Jana Liebenthron
Section II Clinical Procedures
31 Ultrasound Evaluation in Treatment Management
78(2)
Ippokratis Sarris
Geeta Nargund
32 Three-Dimensional Ultrasound and the Diagnosis of Uterine Anomalies
80(2)
Magued Adel Mikhail
Megan Lively
Candice P. Holliday
Botros Rizk
33 Infertility Ultrasound Evaluation: One-Stop Procedure
82(4)
Spiros A. Liatsikos
Geeta Nargund
34 Office Hysteroscopy
86(2)
Linda D. Bradley
35 Hysteroscopic Management of Uterine Septum
88(4)
Omar Abuzeid
Mostafa Abuzeid
36 Hysteroscopic Lysis of Intrauterine Adhesions
92(2)
Bolonduro Oluwamuyiwa
Mostafa Abuzeid
37 Hysteroscopic Management of Submucosal Fibroids
94(4)
Salem Joseph
Mostafa Abuzeid
38 Saline Infusion Sonohysterography
98(2)
Alan Bolnick
Mostafa Abuzeid
39 General Principles of Laparoscopy
100(4)
Rubin Raju
Mostafa Abuzeid
40 Microlaparoscopy
104(2)
Oscar D. Almeida, Jr.
41 Laparoscopic Myomectomy and Mini-Laparotomy: An Alternative Approach for Myomectomy
106(2)
Ahmed Abdelaziz
Mostafa Abuzeid
42 Laparoscopic Excision of a Large Endometrioma
108(2)
Rubin Raju
Mostafa Abuzeid
43 Laparoscopic Management of Subtle Fimbrial Pathology Secondary to Endometriosis
110(2)
Omar Abuzeid
Mostafa Abuzeid
44 Laparoscopic Management of Mature Cystic Teratoma
112(2)
Rahima Sanya
Mostafa Abuzeid
45 Tubal Cannulation for Proximal Tubal Disease
114(2)
Wael Salem
Osama A.H. Abu Zinadah
Joshua Ekladios
Botros Rizk
46 Robotic Assisted Laparoscopic Myomectomy
116(2)
Hashem Lotfy
Arsany Bassily
Lauren Mann
Botros Rizk
47 Robotic Assisted Tubal Anastomosis after Tubal Sterilization
118(2)
Hashem Lotfy
Rola Turki
Botros Rizk
48 Unexplained Infertility
120(2)
Hassan N. Sallam
49 Male Evaluation for Infertility
122(2)
Kiranpreet Khurana
Edmund Sabanegh, Jr.
Ashok Agarwal
50 Hysterosalpingography
124(4)
Islam Fahmi
Mostafa Abuzeid
Shawky Z.A. Badawy
51 Intrauterine Insemination: Clinical Perspectives
128(4)
Willem Ombelet
52 GnRH Agonist to Trigger Final Follicular Maturation in IVF
132(2)
Nayana Talukdar
Robert F. Casper
53 GnRH Agonist Protocols
134(2)
Justin Tan
Rabea Youcef Khoudja
Michael Dooley
Michael H. Dahan
Seang Lin Tan
54 GnRH Agonists before IVF for Advanced Endometriosis
136(2)
Hassan N. Sallam
Nooman H. Sallam
55 Microdose GnRH Agonist Flare Protocol
138(2)
Magued Adel Mikhail
Wael Salem
Botros Rizk
56 Gonadotropin-Releasing Hormone Antagonist Cycle for In Vitro Fertilization
140(2)
Michael H. Dahan
Justin Tan
Baris Ata
Rabea Youcef Khoudja
Seang Lin Tan
57 Minimal Stimulation for IVF
142(2)
Ippokratis Sarris
Geeta Nargund
58 Ovarian Hyperstimulation Syndrome
144(4)
Magued Adel Mikhail
Daniel Antonious
Candice P. Holliday
Botros Rizk
59 The Prediction of OHSS
148(2)
Jan Gerris
Jana Claeys
60 Tips on Technique of Embryo Transfer
150(2)
Omar Abuzeid
Mostafa Abuzeid
61 Difficult Embryo Transfers
152(4)
Gautam N. Allahbadia
Goral N. Gandhi
62 In Vitro Maturation of Oocytes (IVM) for Fertility Treatment and Fertility Preservation
156(4)
Michael H. Dahan
Justin Tan
Rabea Youcef Khoudja
Andrew Mok
Seang Lin Tan
63 Complications and Outcome of IVF
160(2)
Jan Gerris
64 Obstetric Complications after In Vitro Fertilization
162(2)
Brian Brocato
David F. Lewis
65 Twins Pregnancy after ART
164(2)
David F. Lewis
Brian Brocato
66 IVF and Endometriosis
166(2)
Graciela Kohls Ilgner
Juan Antonio Garcia-Velasco
67 Leiomyoma of the Uterus
168(2)
Shawky Z.A. Badawy
68 Office Management of Polycystic Ovarian Syndrome
170(2)
Shawky Z.A. Badawy
69 Adenomyosis: Imaging and Treatment
172(2)
Magued Adel Mikhail
Candice P. Holliday
Botros Rizk
70 Ectopic Pregnancy: Evaluation and Management
174(3)
James M. Shwayder
Index 177
Botros Rizk, MD, FRCOG, FRCS, HCLD, FACOG, FACS, is Professor and Head of Reproductive Endocrinology and Infertility, Medical and Scientific Director of In Vitro Fertilization and Assisted Reproduction, University of South Alabama, Mobile, Albama, USA; Distinguished Adjunct Professor of Reproductive Endocrinology and Infertility, Obstetrics and Gynecology, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia; and Distinguished Adjunct Scientific Director, IVF Michigan, Rochester Hills, Michigan, USA

Markus Montag, PhD, is Professor and CEO, ilabcomm GmbH, Sankt Augustin, NRW, Germany