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E-raamat: Stem Cells and Aging: Methods and Protocols

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Stem Cells and Aging: Methods and ProtocolsSuurem pilt
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This fully updated book collects protocols that reflect current investigations into how stem cell populations change during organismal aging. These methodologies are well-established and described in easy-to-follow fashion so as to be valuable for not only experts but also novices in this ever-developing area of stem cell research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

 

Authoritative and up-to-date, Stem Cells and Aging: Methods and Protocols, Third Edition serves as an ideal guide for researchers working toward furthering our understanding of the aging process.

Protocols to Assess the Aging of Human Mesenchymal Stem Cells.-
Analyzing Muscle Stem Cell Function Ex Vivo.- Tubular Aggregates as a Marker
of Aging in Skeletal Muscle.- Fusion of Human Synovium-Derived Mesenchymal
Stem/Stromal Cells with Primary Human Chondrocytes Using the Modified
Adherence Method (MAM).- Multiplex PCR for the Rapid Diagnosis of
Myeloproliferative Neoplasms.- Ex Vivo Expansion of Cord Blood Hematopoietic
Stem and Progenitor Cells.- Evaluation of Anti-Aging Agents Using the
D-Galactose-Induced Accelerated Aging Model.- Methods to Detect and Compare
Cellular and Mitochondrial Changes in Senescent and Healthy Mesenchymal Stem
Cells.- Transfer of Mitochondria from Healthy Stem Cells to Injured Cells in
Stroke with Retinal Impairments.- A Protocol for Detecting DNA Methylation
Changes at CpG Sites of Stemness-Related Genes in Aging Stem Cells.- Robust
Expansion of Hematopoietic Stem Cells Ex Vivo Using Small Molecule
Cocktails.- A Genome-Wide CRISPR/Cas9 Screen Identifies Regulatory Genes for
Stem Cell Aging.- Immunocompetent  Brain  Organoids  with  Microglia  Allow 
Advanced  Aging  Research.- Quality Control in Human Adipose-Derived
Stromal/Stem Cells and Tissue Engineering Fat Models for Aging Studies.-
Mesenchymal Stromal Cell (MSC) Isolation and Induction of Acute and
Replicative Senescence.- Delivery of Stem Cell Rejuvenating Compounds via
Subcutaneous Osmotic Pumps.- Isolation Procedure for Rat Pancreatic Ductal
Cells.- Polychromatic Flow Cytometry to Identify Rare Aged Hematopoietic Stem
Cell Subpopulations.- Isolation of Human Hematopoietic Stem Cells from an
Apheresis Sample.- Quantifying Muscle Regeneration: Activated Muscle
Satellite Cells and New Regenerated Myofibers in Chronic and Acute
Degeneration Models.- Identification of De Novo Dividing Stem Cells.- Rat
Ductal Cell-Derived Differentiation into Islet-Like Cells.
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