This fully updated book collects protocols that reflect current investigations into how stem cell populations change during organismal aging. These methodologies are well-established and described in easy to follow fashion so as to be valuable for not only experts but novices in the ever-developing stem cell field. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Stem Cells and Aging: Methods and Protocols, Second Edition serves as an ideal guide for all researchers working in this very active area of study.
Assessing Muscle Stem Cell Clonal Complexity during Aging.- Simultaneous
Isolation of Stem and Niche Cells of Skeletal Muscle: Applicability for Aging
Studies.- Isolation and Culture of Individual Myofibers and Their Adjacent
Muscle Stem Cells from Aged and Adult Skeletal Muscle.- Methods and
Strategies for Procurement, Isolation, Characterization, and Assessment of
Senescence of Human Mesenchymal Stem Cells from Adipose Tissue.- Quantifying
Senescence-Associated Phenotypes in Primary Multipotent Mesenchymal Stromal
Cell Cultures.- Adipogenic and Osteogenic Differentiation of In Vitro Aged
Human Mesenchymal Stem Cells.- Human Skeletal-Muscle-Derived Mesenchymal
Stem/Stromal Cell Isolation and Growth Kinetics Analysis.- Complete
Assessment of Multilineage Differentiation Potential of Human
Skeletal-Muscle-Derived Mesenchymal Stem/Stromal Cells.- Human
Synovium-Derived Mesenchymal Stem Cells: Ex Vivo Analysis.- 3D-Embedded Cell
Cultures to Study Tendon Biology.- Targeted, Amplicon-Based,Next-Generation
Sequencing to Detect Age-Related Clonal Hematopoiesis.- Column Free Method
for Isolation and Culture of C-Kit Positive Stem Cells from Atrial Explants.-
Histological Assessment of Cre/loxP Genetic Recombination in the Aging
Subventricular Zone of Nestin-CreERT2/Rosa26YFP Mice.- Infrared Spectroscopy
and Imagng in Stem Cells and Agng Research.- Use of U-STELA for Accurate
Measurement of Extremely Short-Telomeres.- Surface-Antigen-Based
Identification of In Vitro Expanded Skeletal-Muscle-Derived Mesenchymal
Stromal/Stem Cells Using Flow Cytometry.- Analysis of Stem Cells and Their
Activity in Human Skeletal Muscles by Immunohistochemistry.- Methods for
Detection of Autophagy in Mammalian Cells.- Metabolomic and Proteomic
Analyses of Mouse Primordial Germ Cells.- Reprogramming of Aged Cells into
Pluripotent Stem Cells by Nuclear Transfer.- Generation of Retinal Pigmented
Epithelial (RPE) Cells for Treatment of Age-Related Macular Degeneration
(AMD).- Histopathological and Behavioral Assessments of Aging Effects on Stem
Cell Transplants in an Experimental Traumatic Brain Injury.- 3D Age-Specific
Mortality Trajectory: A Survival Analysis Protocol.- Isolation, Expansion,
and Characterization of Whartons Jelly-Derived Mesenchymal Stromal Cell:
Method to Identify Functional Passages for Experiments.- CRISPR Base Editing
in Induced Pluripotent Stem Cells.
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