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E-raamat: Suicide Gene Therapy: Methods and Protocols

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Suicide Gene Therapy: Methods and ProtocolsSuurem pilt
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This detailed volume explores the methods used for most of the recent approaches to suicide gene therapy of cancer, which exploits promoters that are specific to cancer cells, thereby ensuring (or greatly increasing the likelihood) that the therapeutic gene is expressed only in cancer cells. The book also contains chapters describing methods to improve the safety of cell therapy and techniques utilizing bone marrow mesenchymal cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  

Authoritative and practical, Suicide Gene Therapy: Methods and Protocols serves as an ideal guide for researchers expanding upon our knowledge and application of this vital form of cancer therapy.
Origins of Suicide Gene Therapy.- Cancer Suicide Gene Therapy with TK.007.- Osteonectin Promoter-Mediated Suicide Gene Therapy of Prostate Cancer.- Suicide Gene Therapy for Oral Squamous Cell Carcinoma.- Generation of Suicide Gene-Modified Chimeric Antigen Receptor-Redirected T-Cells for Cancer Immunotherapy.- Mesenchymal Stem Cell Exosome-Mediated Prodrug Gene Therapy for Cancer.- Extracellular Vesicles as Carriers of Suicide mRNA and/or Protein in Cancer Therapy.- Production of Viral Vectors with Suicide Genes by Utilizing the Intron-Splicing Mechanism of Insect Cells.- Resveratrol-Responsive CArG Elements from the Egr-1 Promoter for the Induction of GADD45a to Arrest the G2/M Transition.- Non-Invasive Monitoring of Suicide Gene Therapy by Using Multimodal Molecular Imaging.- In Vitro Assay for the Evaluation of Cytotoxic Effects Provided by a Combination of Suicide and Killer Genes in a Bicistronic Vector.- Engineered Thymidine-Active Deoxycytidine Kinase for Bystander Killing of Malignant Cells.- Photochemical Internalization Enhanced Non-Viral Suicide Gene Therapy.- Suicide Gene Therapy of Oral Squamous Cell Carcinoma and Cervical Carcinoma In Vitro.

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