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E-raamat: Super-Resolution Imaging in Biomedicine

Edited by (Istituto Italiano di Tecnologia, Genova, Italy), Edited by (Maastricht University, The Netherlands)
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This book encompasses the full breadth of the super-resolution imaging field, representing modern techniques that exceed the traditional diffraction limit, thereby opening up new applications in biomedicine. It shows readers how to use the new tools to increase resolution in sub-nanometer-scale images of living cells and tissue, which leads to new information about molecules, pathways and dynamics. The book highlights the advantages and disadvantages of the techniques, and gives state-of-the-art examples of applications using microscopes currently available on the market. It covers key techniques such as stimulated emission depletion (STED), structured illumination microscopy (SSIM), photoactivated localization microscopy (PALM), and stochastic optical reconstruction microscopy (STORM). It will be a useful reference for biomedical researchers who want to work with super-resolution imaging, learn the proper technique for their application, and simultaneously obtain a solid footing in other techniques.

Arvustused

"an outstanding book suitable for people with interests in the biomedical applications of super-resolution microscopy. The chapter on structured-light microscopy is another gem, with its exposition of theory, instrumentation and limitations. The warning of artifacts in the specimen preparation, the image acquisition, as well as image analysis and interpretation, permeate the books content. The figures are all high quality" Optics & Photonics News (Mar 2017), review by Barry R. Masters "an outstanding book suitable for people with interests in the biomedical applications of super-resolution microscopy. The chapter on structured-light microscopy is another gem, with its exposition of theory, instrumentation and limitations. The warning of artifacts in the specimen preparation, the image acquisition, as well as image analysis and interpretation, permeate the books content. The figures are all high quality" Optics & Photonics News (Mar 2017), review by Barry R. Masters

"The uniformly excellent in-depth presentations speak to the expertise and leadership of the editors who should be congratulated for ensuring the contents of this book are complete while also avoiding unnecessary repetition. Those researchers interested in adding super-resolution techniques to their imaging rigs could hardly do better than reading this book." Contemporary Physics (Jan 2018)

Series Preface xi
Preface xiii
Editors xv
Contributors xvii
Section I Overview of Super-Resolution Technologies
1 In the Realm of Super-Resolved Fluorescence Microscopy
3(16)
Alberto Diaspro
Paolo Bianchini
Giuseppe Vicidomini
Colin J.R. Sheppard
Luca Lanzano
Francesca Cella Zanacchi
Lauretta Galeno
2 Super-Resolution in a Standard Microscope: From Fast Fluorescence Imaging to Molecular Diffusion Laws in Live Cells
19(30)
Carmine Di Rienzo
Enrico Gratton
Fabio Beltram
Francesco Cardarelli
3 Photoswitching Fluorophores in Super-Resolution Fluorescence Microscopy
49(30)
Ulrike Endesfelder
4 Image Analysis for Single-Molecule Localization Microscopy
79(20)
Martin Ovesny
Pavel Krizek
Josef Borkovec
Zdenek Svindrych
Guy M. Hagen
5 Deconvolution of Nanoscopic Images
99(26)
Hans van der Voort
Section II The Methods
6 Re-Scan Confocal Microscopy
125(12)
Giulia M.R. De Luca
Ronald M.P. Breedlijk
Erik M.M. Manders
7 Structured Illumination Microscopy
137(12)
Sara Abrahamsson
Graeme Ball
Kai Wicker
Rainer Heintzmann
Lothar Schermelleh
8 Correlative Nanoscopy: AFM Super-Resolution (STED/STORM)
149(24)
Jenu Chacko
Francesca Cella Zanacchi
Benjamin Harke
Alberto Diaspro
Claudio Canale
9 Stochastic Optical Fluctuation Imaging
173(16)
Simon Christoph Stein
Anja Huss
Ingo Gregor
Jorg Enderlein
10 Super-Resolution Two-Photon Excitation Microscopy Utilizing Transmissive Liquid Crystal Devices
189(26)
Kohei Otomo
Terumasa Hibi
Yuichi Kozawa
Sari Ipponjima
Shunichi Sato
Tomomi Nemoto
11 Fluorescent Nanodiamonds in Biological and Biomedical Imaging and Sensing
215(26)
Metin Kayci
Flavio M. Mor
Aleksandra Radenovic
Section III Applications in Biology and Medicine
12 Optical Nanoscopy with SOFI
241(18)
Xuanze Chen
Zhiping Zeng
Xi Zhang
Pingyong Xu
Peng Xi
13 Super-Resolution Fluorescence Microscopy of the Nanoscale Organization of RNAP and DNA in E. coli
259(22)
Christoph Spahn
Ulrike Endesfelder
Mike Heilemann
14 Correlative Live-Cell and Super-Resolution Microscopy and Its Biological Applications
281(20)
Melike Lakadamyali
15 SAX Microscopy and Its Application to Imaging of 3D-Cultured Cells
301(20)
Katsumasa Fujita
Kentaro Mochizuki
Nicholas I. Smith
16 Quantitative Super-Resolution Microscopy for Cancer Biology and Medicine
321(36)
Alec Peters
Andrew Nickerson
Li-Jung Lin
Xiaolin Nan
17 Distribution, Organization, and Dynamics of EGF Receptor in the Plasma Membrane Studied by Super-Resolution Imaging
357(26)
Susana Rocha
Herlinde De Keersmaecker
Eduard Fron
Mitsuhiro Abe
Asami Makino
Hiroshi Uji-i
Johan Hofkens
Toshihide Kobayashi
Atsushi Miyawaki
Hideaki Mizuno
18 Super-Resolution Microscopy: The Dawn of a New Era in Cardiovascular Research
383(32)
Marc A.M.J. van Zandvoort
Index 415
Alberto Diaspro, Ph.D, is a professor of applied physics and head of the Nanophysics Unit of the Italian Institute of Technology. He also founded the Laboratory for Advanced Microscopy, Bioimaging, and Spectroscopy (LAMBS).

Marc A.M.J. van Zandvoort, Ph.D, is the leader of the Two-Photon Core Facility in Aachen and head of the Advanced Microscopy Unit in the Department of Genetics and Cell Biology at Maastricht University, Netherlands.