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E-raamat: T-Cell Motility: Methods and Protocols

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T-Cell Motility: Methods and ProtocolsSuurem pilt
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This volume discusses the latest developments in cellular, molecular, biochemical, and imaging assays to study the biology and functions of T-cells. The chapters in this book cover topics such as LFA-1/ICAM-1 interactions in T-cell motility; using 3D-SIM to dissect signaling cross-talks in motile T-cells; GapmeR-mediated gene silencing in motile T-cells; activity of cellular kinases in migrating T-cells; and computational analysis of protein-protein interactions in motile T-cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, T-Cell Motility: Methods and Protocols is an essential resource for graduate students, postdoctoral fellows, and principal investigators working in the fields of immunology, T-cell biology, biochemistry, molecular biology, and imaging.


An Introduction to LFA-1/ICAM-1 Interactions in T-Cell
Motility.- Isolation of Human Peripheral Blood T Lymphocytes.- A Laboratory
Model to Study T-Cell Motility.- Quantification of T-Cell Migratory
Phenotypes using High Content Analysis.- Live Cell Imaging and Analysis to
Capture T-Cell Motility in Real-Time.- Three-Dimensional Structured
Illumination Microscopy (3D-SIM) to Dissect Signalling Cross-Talks in Motile
T-Cells.- Real-Time Impedance-Based Detection of LFA-1-Stimulated T-Cell
Transwell Chemotaxis.- Quantitative Real-Time PCR for Evaluating
Transcriptional Changes in T-Lymphocytes.- GapmeR-Mediated Gene Silencing in
Motile T-Cells.- Live Imaging of Resident T-Cell Migration in Human Lymphoid
Tissue Slices using Confocal Microscopy.- Phosphoprotein Enrichment for
Protein Analysis in Motile T-Lymphocytes.- Immunometabolomic Phenotyping of
Motile T-Cells.- Profiling Activity of Cellular Kinases in Migrating
T-Cells.- Biotinylation Assay to Determine LFA-1 Recycling in Motile
T-Lymphocytes.- A Protocol to Study T-Cell Signalling in an Immune Synapse by
Microscopy.- Enzyme-Linked Immunosorbent Assay for T-Cell Dependent
Immunogenicity Assessment of Therapeutic Peptides.- Modified Intravital
Microscopy to Assess Vascular Health and T-Cell Motility.- Computational
Analysis of Protein-Protein Interactions in Motile T-Cells.
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