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E-raamat: Thin Layer Chromatography in Phytochemistry

Edited by (Wroclaw University of Technology, Poland), Edited by (Medical University of Lublin, Poland), Edited by (Lafayette College, Easton, Pennsylvania, USA)
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Thin layer chromatography (TLC) is increasingly used in the fields of plant chemistry, biochemistry, and molecular biology. Advantages such as speed, versatility, and low cost make it one of the leading techniques used for locating and analyzing bioactive components in plants.

Thin Layer Chromatography in Phytochemistry is the first source devoted to supplying state-of-the-art information on TLC as it applies to the separation, identification, quantification, and isolation of medicinal plant components. Renowned scientists working with laboratories around the world demonstrate the applicability of TLC to a remarkable diversity of fields including plant genetics, drug discovery, nutraceuticals, and toxicology.

Elucidates the role of plant materials in the pharmaceutical industry...

Part I provides a practical review of techniques, relevant materials, and the particular demands for using TLC in phytochemical applications. The text explains how to determine the biological activity of metabolites and assess the effectiveness of herbal medicines and nutritional supplements. Part II concentrates on TLC methods used to analyze specific plant-based metabolite classes such as carbohydrates, proteins, alkaloids, flavonoids, terpenes, etc. Organized by compound type, each chapter discusses key topics such as sample preparation, plate development, zone detection, densitometry, and biodetection.

Demonstrates practical methods that can be applied to a wide range of disciplines...

From identification to commercial scale production and quality control, Thin Layer Chromatography in Phytochemistry is an essential bench-top companion and reference on using TLC for the study of plant-based bioactive compounds.
Preface xiii
Editors xv
Contributors xix
Part I
Overview of the Field of TLC in Phytochemistry and the Structure of the Book
3(12)
Monika Waksmundzka-Hajnos
Joseph Sherma
Teresa Kowalska
Plant Materials in Modern Pharmacy and Methods of Their Investigations
15(22)
Krystyna Skalicka-Wozniak
Jaroslaw Widelski
Kazimierz Gtowniak
Medicines and Dietary Supplements Produced from Plants
37(22)
Anita Ankli
Valeria Widmer
Eike Reich
Primary and Secondary Metabolites and Their Biological Activity
59(18)
Ioanna Chinou
Plant Chemosystematics
77(26)
Christian Zidorn
Sorbents and Precoated Layers for the Analysis and Isolation of Primary and Secondary Metabolites
103(16)
Joseph Sherma
Chambers, Sample Application, and Chromatogram Development
119(56)
Tadeusz H. Dzido
Tomasz Tuzimski
Derivatization, Detection (Quantification), and Identification of Compounds Online
175(18)
Bernd Spangenberg
Biodetection and Determination of Biological Activity of Natural Compounds
193(22)
Erno Tyihak
Agnes M. Moricz
Peter G. Ott
Forced-Flow Planar Layer Liquid Chromatographic Techniques for the Separation and Isolation of Natural Substances
215(40)
Emil Mincsovics
Part II
Primary Metabolites
TLC of Carbohydrates
255(22)
Guilherme L. Sassaki
Lauro M. de Souza
Thales R. Cipriani
Marcello Iacomini
TLC of Lipids
277(22)
Svetlana Momchilova
Boryana Nikolova-Damyanova
Amino Acids
299(32)
Ravi Bhushan
Secondary Metabolites---Shickimic Acid Derivatives
Sample Preparation and TLC Analysis of Phenolic Acids
331(34)
Magdalena Wojciak-Kosior
Anna Oniszczuk
Application of TLC in the Isolation and Analysis of Coumarins
365(40)
Monika Waksmundzka-Hajnos
Mirostaw A. Hawryt
Application of TLC in the Isolation and Analysis of Flavonoids
405(20)
Marica Medic-Saric
Ivona Jasprica
Ana Mornar
Zeljan Males
TLC of Lignans
425(26)
Lubomir Opletal
Helena Sovova
Secondary Metabolites---Isoprenoids
TLC of Mono- and Sesquiterpenes
451(30)
Angelika Koch
Simla Basar
Rita Richter
TLC of Diterpenes
481(38)
Michal L. Hajnos
TLC of Triterpenes (Including Saponins)
519(24)
Wieslaw Oleszek
Ireneusz Kapusta
Anna Stochmal
TLC of Carotenoids
543(32)
George Britton
TLC of Sterols, Steroids, and Related Triterpenoids
575(30)
Laurie Dinan
Juraj Harmatha
Rene Lafont
TLC of Iridoids
605(18)
Grazyna Zgorka
Secondary Metabolites---Amino Acid Derivatives
TLC of Indole Alkaloids
623(18)
Peter John Houghton
TLC of Isoquinoline Alkaloids
641(44)
Monika Waksmundzka-Hajnos
Anna Petruczynik
TLC of Tropane Alkaloids
685(16)
Tomasz Mroczek
TLC of Alkaloids from the Other Biosynthetic Groups
701(56)
Jolanta Flieger
Secondary Metabolites---Compounds Derived from Acetogenine (Acetylocoenzyme A)
Polyacetylenes: Distribution in Higher Plants, Pharmacological Effects, and Analysis
757(60)
Lars P. Christensen
Henrik B. Jakobsen
Quinone Derivatives in Plant Extracts
817(36)
Grazyna Matysik
Agnieszka Skalska-Kaminska
Anna Matysik-Wozniak
Index 853
Monika Waksmundzka-Hajnos, Joseph Sherma, Teresa Kowalska