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E-raamat: Vaccine Technologies for Veterinary Viral Diseases: Methods and Protocols

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This second edition includes a collection of antigen production and delivery strategies for vaccine development in veterinary species. New and updated chapters guide readers through protocols for antigen production, experimental antigen delivery and the analysis of immune responses upon vaccination. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 Authoritative and cutting-edge, Vaccine Technologies for Veterinary Viral Diseases: Methods and Protocols, Second Edition aims to be a useful and practical guide to researches to help further their study in this field. 

Preface v
Contributors ix
1 An Overview of Veterinary Viral Diseases and Vaccine Technologies
1(26)
Alejandro Brun
2 Production and Purification of Candidate Subunit Vaccines by IC-Tagging Protein Encapsulation
27(14)
Natalia Barreiro-Pineiro
Tomds Pose-Boirazian
Rebeca Menaya-Vargas
Jose M. Martinez-Costas
3 Elastin-like Polymers as Nanovaccines: Protein Engineering of Self-Assembled, Epitope-Exposing Nanoparticles
41(32)
Alessandra Girotti
Juan Gonzdlez-Valdivieso
Irene Alonso-Sampedro
Sara Escalera-Anzola
Sandra Ramos-Diez
F. Javier Arias
4 Display of Heterologous Proteins in Bacillus Subtilis Biofilms for Enteric Immunization
73(24)
Claudia Aguilar
Ramona Wissmann
Cornel Fraefel
Catherine Eichwald
5 Production of Influenza H5 Vaccine Oligomers in Plants
97(12)
Hoang Trong Phan
Udo Conrad
6 DNA Vaccines in Pigs: From Immunization to Antigen Identification
109(16)
Francesc Accensi
Laia Bosch-Cambs
Paula L. Monteagudo
Fernando Rodriguez
7 Use of Foot-and-Mouth Disease Virus Non-coding Synthetic RNAs as Vaccine Adjuvants
125(12)
Miguel Rodriguez-Pulido
Miryam Polo
Belen Borrego
Margarita Sdiz
8 Evaluation of Innate Lymphoid Cells and Dendritic Cells Following Viral Vector Vaccination
137(18)
Zheyi Li
Sreeja Roy
Charani Ranasinghe
9 Generation of Replication Deficient Human Adenovirus 5 (Ad5) Vectored FMD Vaccines
155(22)
Gisselle N. Medina
Teresa de los Santos
Fayna Diaz-San Segundo
10 Recombinant Modified Vaccinia Virus Ankara Development to Express VP2, NS1, and VP7 Proteins of Bluetongue Virus
177(18)
Alejandro Martn-Lopez
Sergio Utrilla-Trigo
Luis Jimenez-Cabello
Javier Ortego
11 Cloning Strategies for the Generation of Recombinant Capripoxvirus Through the Use of Screening and Selection Markers
195(14)
Hani Boshra
Mahder Teffera
Jinxing Cao
Shawn Babiuk
12 Using RVFVas a Vector Platform for the Expression of Ruminant Disease Antigens
209(18)
Sandra Moreno
Helen Borrego
Alejandro Brun
13 Generation and Characterization of Single-Cycle Infectious Canine Influenza A Virus (sciCIV) and Its Use as Vaccine Platform
227(30)
Aitor Nogales
Kevin Chiem
Michael Breen
Marta L. DeDiego
Colin R. Parrish
Luis Martinez-Sobrido
14 Reverse Genetics for Influenza A and B Viruses Driven by Swine Polymerase I Promoter
257(46)
Brittany Seibert
Stivalis Cardenas-Garcia
Daniela Rajao
Daniel R. Perez
15 Analysis of the Cellular Immune Responses to Vaccines
283
Nicholas Svitek
Evans L. N. Taracha
Rosemary Saya
Elias Awino
Vish Nene
Lucilla Steinaa
Index 303